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Öğe Examinations on the changes in oviduct epithelium during the sexual cycle by light and electron microscope in Akkaraman sheep(Ankara Univ Press, 2012) Kurum, Aytul; Ozen, AsumanThe aim of the study was to examine the changes in the oviduct epithelium during the sexual cycle by light and electron microscope in Akkaraman sheep. In this study; 10 oestral (follicular phase), 10 luteal and 5 anoestral phase samples were collected from the infundibulum, ampulla and istmus regions of oviduct from a total of 25 healthy Akkaraman sheeps having reached their puberty. In the light microscopy, it was also observed that, in oestral phase; height of the cells were higher than luteal phase and in the luteal phase nucleus extrusion were seen in the ampulla and infundibulum. It was detected that findings of the anoestrus phase were similar to dioestrus phase. Amount of secrets of secretory cells in the ampulla were elevated during the oestral phase which was determined by histochemical stains. During the oestral phase by using electron microscope, three types of granules were seen in the secretory cells which were especially located in the ampulla. First type of granule was electron lucent and had moderately electron dense substance which was located separetely, right below the granule membran. Second type of granule had carried electron dense focus which had consantric lamels around of. Some of these types of granules were also seen located eccentric to electron dense focus. Third type of granules, which don't have electron dense focus at the centre, are with consantric lamels. This study showed the histological changes on the oviduct epithelium of Akkaraman sheeps during sexual cycle. The amount and the characterization of the secret in the secretory cells, varied upon the cycle period and the region of the oviduct.Öğe Histomorphology of the Brunner's Glands in the Angora Rabbit(Medwell Online, 2010) Ergun, Emel; Ergun, Levent; Ozen, Asuman; Kurum, Aytul; Bayraktaroglu, Alev GuerolThe study was aimed to demonstrate the distribution, morphological and histochemical properties of Brunner's glands in the small intestine of the Angora rabbit. The duodenum of 10 healthy animals of both sexes constituted the material of the study. The glands were composed of acini densely packed within the submucosa. The Brunner's glands contained two types of cells, namely, serous and mucous cells. Histochemical examination revealed that the mucous glands and secretory ducts did not react with the Periodic Acid-Schiff (PAS) stain, while serous glands were weakly PAS-positive. Furthermore, muscous glands reacted positively with alcian blue pH 2.5. When applied the combined aldehyde fuchsin-alcian blue pH 2.5 staining procedure, mucous glands were determined to be aldehyde fuchsin (-) and alcian blue (+). These results showed that while a limited amount of neutral carbohydrates was secreted in serous glands, the secretion of the ducts and mucous cells of the duodenal glands in the Angora rabbit was composed of acidic carbohydates with this staining properties of the duodenal secretion. Electron microscopic examination revealed the cytoplasm of mucous gland cells to be filled with electron light secretion granules. Fewer electron dense granules were determined to be present among these electron light granules. The electron dense granules were found within the apical cytoplasm of serous glands.Öğe Histomorphology of the oviduct epithelium in the Angora rabbit(Scientific Technical Research Council Turkey-Tubitak, 2010) Ozen, Asuman; Ergun, Emel; Kurum, AytulThe present study was undertaken to investigate cyclic changes in the structure of oviduct epithelial cells, and the content of oviductal mucus in the Angora rabbit using light and electron microscopy. Ten female Angora rabbits, 5 of which were in the estrual stage and the other 5 were in the luteal stage of the estrous cycle, were used in the study. Tissue samples taken from the fimbria, ampulla and isthmus of the oviduct were examined under light and electron microscope. Ciliated cells were demonstrated to be predominant in the fimbria and ampulla, whereas secretory cells were determined to be most numerous in the isthmus. The estrual stage was characterized with greater cell heights, and increased numbers of ciliated cells and secretion. Both secretion and cilia were determined to decrease evidently in the luteal stage. Neutral and acidic mucosubstances were found to be present in the ampulla and isthmus. In the isthmus, in which secretion was dense, acidic mucosubstance was determined to contain sulphate and carboxyl groups by means of combined Aldehyde fuchsin/Alcian blue (AF/Ab) staining. Electron microscopic examination revealed the presence of electron-dense and electron-light secretion granules in secretory cells. In all cyclic stages, electron-dense foci were observed in the electron-light secretion granules of some secretory cells.Öğe Investigation of Mast Cell Distribution in the Ovine Oviduct During Oestral and Luteal Phases of the Oestrous Cycles(Kafkas Univ, Veteriner Fakultesi Dergisi, 2014) Kurum, Aytul; Ozen, Asuman; Karahan, Siyami; Ozcan, ZiyaMast cells are heterogeneous cell populations that play significant roles in many organs and systems and involve various physiological processes. We aimed to evaluate mast cells in the ovine oviduct mucosa by means of their staining and ultrastructural characteristics. The ovine oviduct samples of Akkaraman breed were collected from the slaughterhouse and they are categorized as luteal and oestral phases. They were fixed either with 10% formalin or IFAA and stained with Toluidine blue and Alcian blue and Safranin O (Ab/SO). Mast cells were located near blood vessels and basal membrane. Compared to 10% formalin fixed tissues, the number of mast cells were higher in IFAA fixed tissues (P=0.003). Importantly all mast cells Ab(+) and SO(-) so that they were categorized as mucosal type. The number of mast cells did not differ between luteal and oestral phases (P>0.05). However, there were significant differences among different regions of the oviduct with a less count in the isthmus regions (P=0.006). Transmission electron microscopy revealed that the oviduct mast cells contained two types of granules: an electron lucent, electron dense. Some electron lucent granules contained an eccentrically located crystal-like structure. The significance of less mast cell counts in the isthmus and the eccentrically located single crystal-like structure should be further investigated in future studies.Öğe Light and electron microscopic studies on the oviduct epithelium of the Pekin duck (Anas platyrhynchos)(Ankara Univ Press, 2009) Ozen, Asuman; Ergun, Emel; Kurum, AytuelThe present study was undertaken to investigate the histochemical properties and light and electron microscopic morphology of the oviduct of laying Pekin ducks and Pekin ducks in the quiescent phase of the reproductive Cycle. The oviducts of five healthy, two-year-old adult laying Pekin ducks and five five-month-old Pekin ducks in the quiescent phase of the reproductive cycle, obtained from the Farm of Ankara University Faculty of Veterinary Medicine, constituted the Material of the present study. The oviduct of the Pekin duck was segmented into five regions. namely, the infundibulum, magnum, isthmus, uterus and vagina. Along the oviduct, the tunica mucosa forms convolutions protruding into the lumen, and the height of these convolutions were determined to increase in the regions of the magnum and uterus. The lamina epithelialis was ascertained to be composed of ciliated and secretory cells. The lamina propria was filled with glands in the magnum, isthmus and uterus. In laying ducks, the PAS-positive reaction in the isthmus and the performic acid/Alcian blue pH 2.5 reaction in the magnum were determined to tic very strong. Oil the other hand, due 10 the cells forming the lamina epithelialis and the proprial glands not having developed fully, these reactions were observed to be weak in ducks in the quiescent phase of the reproductive cycle. In electron microscopic examinations, a single type of secretion granule, of medium electron density and varying size, was observed in the secretory cells of the lamina epithelialis. Electron dense secretion granules were present in the proprial glands of the magnum and isthmus, whereas proprial glands of the Uterus contained electron light granules.Öğe Studies on the morphology, histochemistry and ultrastructure of Paneth cells in the small intestine of the Angora rabbit(Ankara Univ Press, 2009) Ergun, Emel; Ergun, Levent; Ozen, Asuman; Kurum, AytulThe present study was undertaken to determine the morphology, histochemical properties, localization and quantitative distribution of Paneth cells in the small intestine of the Angora rabbit. Tissue samples taken from the duodenum. jejunum and ileum of 10 healthy Angora rabbits, obtained from private breeders, constituted the material of the study. The Paneth cells, which were determined to be located within the crypts of Lieberkuhn, were identified on their basally located nucleus and apically located acidophilic granules. These granules gave positive reactions with Mallory's triple staining, technique as well as with the application of Phloxine-tartrazine, Alcian blue-performic acid and Mallory's phosphotungstic acid-hematoxylin method. Staining with periodic acid-Schiff (PAS). Alcian blue (pH 2.5), and PAS-Alcian blue gave negative reactions. Paneth cells were determined not to display a uniform distribution throughout the small intestine and cell numbers were ascertained to show a gradual increase from the duodenum towards the ileum. The difference between the three regions of the small intestine was determined to he statistically significant (p<0.01). Electron microscopic examination revealed the presence of electron-dense and homogenous granules in the apical cytoplasm of some Paneth cells, whereas homogenous granules of different electron density existed in some other cells.
