Yazar "Pestil, Zuleyha" seçeneğine göre listele

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    Antibodies to Schmallenberg virus in domestic livestock in Turkey
    (Springer, 2013) Azkur, Ahmet Kursat; Albayrak, Harun; Risvanli, Ali; Pestil, Zuleyha; Ozan, Emre; Yilmaz, Oktay
    This, partly retrospective study, was designed to determine the seroprevalence of Schmallenberg virus (SBV), a new Orthobunyavirus first reported in Germany in late 2011, in domestic ruminants from the Middle Black Sea, West, and Southeast regions of Turkey. An indirect enzyme-linked immunosorbent assay was used to screen serum samples collected from slaughterhouse animals between 2006 and 2013. The overall seroprevalence was 335/1,362 (24.5 %) with 325/816 (39.8 %), 5/307 (1.6 %), 3/109 (2.8 %), and 2/130 (1.5 %) recorded in cattle, sheep, goats, and Anatolian water buffalo, respectively. This is the first study to demonstrate the presence of antibodies to SBV in Turkish ruminants; it indicates that cattle are more susceptible to infection than sheep, goats, or buffalo and that exposure of domestic ruminants to SBV in Turkey may have occurred up to 5 years prior to the first recorded outbreak of the disease in 2011.
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    Circulation of Schmallenberg virus in Turkey, 2013
    (Scientific Technical Research Council Turkey-Tubitak, 2016) Tonbak, Sukru; Azkur, Ahmet Kursat; Pestil, Zuleyha; Biyikli, Emel; Abayli, Hasan; Baydar, Ersoy; Bulut, Hakan
    Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1%) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98%-99% nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.
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    A Large-Scale Outbreak of Bovine Ephemeral Fever in Turkey, 2012
    (Japan Soc Vet Sci, 2013) Tonbak, Sukru; Berber, Engin; Yoruk, Mustafa Deniz; Azkur, Ahmet Kursat; Pestil, Zuleyha; Bulut, Hakan
    Regional cases of bovine ephemeral fever (BEF) were documented previously in Turkey. Previous cases were confirmed in South-East Turkey with low cow mortality. Recent BEF-suspected outbreaks with high mortality were documented in many regions of Turkey in 2012. The aim of study was the epidemiological examination of the outbreak and molecular characterization of the viruses detected from the outbreak. For this reason, blood samples were collected from BEF-suspected outbreak regions. From the results of RT-PCR, high rate of BEF-suspected samples (48/60 or 80%) was found positive for BEF virus (BEFV) RNA. The nucleotide sequences of the G(1) region of G gene of BEFV in the current study during the 2012 outbreak were grouped into cluster II of BEFV. It was suggested that BEFV may be spread out to other neighbor countries in the future years.