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  • Küçük Resim
    Öğe
    Effect of Fullerenol C60 on Erythrocyte Deformability During Ischaemia-Reperfusion Injury of Lower Extremity in Diabetic Rats
    (Gazi Univ, Fac Med, 2019) Kip, Gülay; Kartal, Hakan; Çomu, Faruk Metin; Polat, Yücel; Arslan, Mustafa; Küçük, Ayşegül
    Background: Fullerenol, a water-soluble C60-fullerene derivative synthesized by Chiang et al, has been demonstrated to be able to scavenge free radicals in vitro and in vivo. Although its protective effects have been already studied and shown in ischemia reperfusion (IR) injury, additional investigation is necessary for its effect on erythrocyte deformability. The purpose of our study was to look into the effects of fullerenol C60 on erythrocyte deformability in rat lower extremity ischemia reperfusion injury model. Materials and Methods: After approval of the Ethics Committee, 30 Wistar Albino rat were divided into 5 groups (n:6) as; Control (C), Diabetes (group D), diabetes+ fullerenol C60 group (DF), diabetes+ IR (group DIR) and diabetes IR+ fullerenol C60 (DIRF). 55 mg/kg streptozotocin was administered to the rats for diabetes. After the period of 72 hour, blood glucose concentration was mesured, 250 mg/dl and above were considered as diabetic rat. Four week after the formation of diabetes, rats were subjected to 2 hour ischemia and 2 hour reperfusion. Erythrocyte packs were prepared from heparinized blood samples and deformability measurements were performed. Results: The deformability index was significantly increased in diabetic rats; however, it was similar in group D, DF and DIRF. It was significantly increased in group DIR when compared to group C, D, DF and DIRF. The relative resistance was increased in I/R models. Conclusion: This study aimed to investigate the effects of IR on erythrocyte deformability which may lead to disturbance in blood flow and hence tissue perfusion in infrarenal rat aorta. We found that fullerenol C60 had beneficial effects by reversing undesirable effects of IR. In our opinion, further studies with larger volume are required to support our promising results.
  • Küçük Resim
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    The Effect of Picroside-2 on Erythrocyte Deformability and Lipid Peroxidation in StreptozotocinInduced Diabetic Rats Subjected to Left Anterior Descending Artery-Ischaemia Reperfusion
    (2017) Çomu, Faruk Metin; Polat, Yücel; Özer, Abdullah; Erer, Dilek; Kirişçi, Mehmet; Dursun, Ali Doğan; Tatar, Tolga
    Aim: Diabetes mellitus (DM) is a chronic metabolic disorder principally characterized by an elevation in oxidative stress levels. Ischaemia-reperfusion (IR) injury starts a cascade of events that lead to tissue ischaemia and cellular damage produced by reperfusion causing an inflammatory like response. Erythrocyte deformability and plasma viscosity are important clinical implications for organ and tissue perfusion. Recent studies have found that picroside-2 has antioxidant, neuroprotective and anti-inflamatory effects. The aim of our study was to investigate the effects of picroside-2 on erythrocyte deformability and lipid peroxidation in streptozotocin-induced diabetic rats subjected to left anterior descending (LAD) artery IR. Methods: The animals were randomly assigned to one of five experimental groups. In Group (control) C, DC (diabetes-control group), and DP (diabetespicroside-2 group) neither coronary artery occlusion nor reperfusion were performed in the control rats. In Group DIR, a branch of the LAD artery was occluded for 45 minutes followed by 90 minutes of reperfusion to produce IR. In Group DIRP, picroside-2 was administrated via 10 mg.kg-1 inraperitoneal (IP) route 30 minutes before ligating the LAD artery. Serum malondialdehyde and nitric oxide activities were investigated to document lipid peroxidation and erythrocyte deformability index. Results: Deformability index was notably increased in diabetic rats (p<0.0001). It was notably increased in Group DIR when compared to Group C, DC, DP and DIRP (p<0.0001, p=0.009, p=0.013, p=0.009, respectively). MDA level and NO activity were also higher in IR group than the other groups. Conclusion: Erythrocyte deformability index was decreased in rats with diabetes and IR injury. This injury may lead to further microcirculatory problems. Picroside 2 was shown to be useful in reducing the side effects of this kind of injury.