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    Anti Wnt-1 Monoclonal Antibody's Conjugated with Gold Nanoparticles, Induced Apoptosis on MCF-7 Breast Cancer Cell Lines
    (Trans Tech Publications Ltd, 2019) Azar, Mehdi Tayybi; Saglam, Necdet; Turk, Mustafa
    Wnt-beta Catenin Pathway has an important role in many cancers. Wnt-1 protein from Wnt protein family that regulate this pathway has a special effect on the development of breast cancer. Monoclonal antibodies attach to metal nanoparticles have an important role in diagnosis and treatment of cancers. In this study, Anti Wnt-1 monoclonal antibody was conjugated to the gold nanoparticles synthesized by Turkevich method. Conjugation was achieved using EDC-NHS method. The density of the monoclonal antibodies that bonded to gold nanoparticles was measured by Roche Cobas Integra 400 Plus device. MCF-7 breast cancer cell line was treated with conjugated nanoparticles for 48 h then Double Staining method was used to detect apoptosis cells. The results showed that inhibition of Wnt-1 protein in extracellular matrix causes apoptosis and gold nanoparticles have a positive effect on Anti Wnt-1 monoclonal antibodies and this positive effect causes apoptosis to increase rate in conjugating nanoparticles.
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    A disposable gold-cellulose nanofibril platform for SERS mapping
    (ROYAL SOC CHEMISTRY, 2020) Tanis, Saliha Nur; İlhan, Hasan; Güven, Burcu; Tayyarcan, Emine Kübra; Çiftçi, Hakan; Saglam, Necdet; Hakki Boyaci, Ismail
    In this study, we present a disposable and inexpensive paper-like gold nanoparticle-embedded cellulose nanofibril substrate for the rapid enumeration ofEscherichia coli(E. coli) using surface-enhanced Raman scattering (SERS) mapping. A disposable SERS substrate was simply constructed by mixing CNF and gold chloride solution at 120 degrees C in a water bath. The application of the resulting substrate was carried out by enrichment and SERS detection ofE. coli. To this end, the spherical gold nanoparticle-embedded cellulose nanofibril substrate was used as a scavenger forE. coli. After the target bacteriaE. coliwere separated from the matrixviaoriented antibodies, the sandwich assay procedure was carried out using 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB)-coated Au nanorod particles that acted as SERS mapping probes. The distribution density of DTNB was demonstrated visually using SERS mapping, and the assay was completed in one hour. The correlation between theE. coliand SERS mapping signals was found to be linear within the range of 15 cfu mL(-1)to 1.5 x 10(5)cfu mL(-1). The limit of detection for the SERS mapping assay was determined to be 2 cfu mL(-1). The selectivity of the developed method was examined withMicrococcus luteus(M. luteus),Bacillus subtilis(B. subtilis), andEnterobacter aerogenes(E. aerogenes), which did not produce any significant response. Furthermore, the developed method was evaluated for detectingE. coliin artificially contaminated samples, and the results were compared with those of the plate-counting method.