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Öğe Glutathione-S-Transferase And P53 Expressions In Human Malignant, Benign And Metastasis Ovarian Tumor Tissues(Wiley, 2019) Dirican, Onur; Kaygin, Pinar; Oguztuzun, Serpil; Erdem, Aysegul; Simsek, Gulcin Guler; Sarialtin, Sezen Yilmaz; Coban, Tulay…Öğe Immunocytochemical Evaluation of Glutathione-S-Transferase P1 Enzyme in Patients with Rheumatoid Arthritis(Wiley, 2019) Dirican, Onur; Kaygin, Pinar; Oguztuzun, Serpil; Simsek, Gulcin Guler; Hayat, Buket; Sarialtin, Sezen Yilmaz; Coban, Tulay…Öğe NLRP3 Expression in Peripheral Blood Mononuclear Cells of Patients with Rheumatoid Arthritis(Society of Pharmaceutical Sciences of Ankara (FABAD), 2023) Sarialtin, Sezen Yilmaz; Küçükşahin, Orhan; Nural, Cemil; Kozaci, Leyla Didem; Kaygin, Pinar; Şimşek, Gülçin Güler; O?uztüzün, SerpilRheumatoid arthritis (RA) is a systemic autoimmune disease characterized by painful, swollen, and inflamed joints. Individual, genetic, and environmental factors influence the development of the disease, which causes involvement not only in the joints but also in other extra-articular tissues. However, the etiopathogenesis of the disease has not yet been fully elucidated. NLRP3, a crucial component of the innate immune system, may contribute to recurrent and chronic inflammation, resulting in inflammation-related diseases. Therefore, we aimed to examine the expression pattern of NLRP3 in peripheral blood mononuclear cells isolated from patients with RA using immunocytochemical approaches in this study. Our findings demonstrated that NLRP3 expression was significantly increased in patients with RA compared to the healthy controls (p<0.01). The highest expression among the patient groups was observed in the active period cases, and this expression considerably increased compared to the control group (p<0.01). Patients in the remission period possessed the lowest expression among the patient groups. Since female gender is considered an independent risk factor for the disease, the effect of gender on expressions was also investigated. NLRP3 was expressed at higher levels in female patients than in males; however, this difference was not significant (p>0.05). The expression patterns of the patient and control groups suggest that NLRP3 may be involved in the development and progression of the disease. To our knowledge, our study is the first research investigating NLRP3 expression profile in peripheral blood mononuclear cells obtained from patients with RA using immunocytochemical approaches. The results of our study highlight significant aspects. However, further research using more sensitive methods with a larger number of cases is required to assess the function of NLRP3 in RA and to provide a deeper insight into the mechanism. © 2023 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.Öğe The Administration of Steroids and its Impact on Caspase-3 Expression in Pediatric Adenoid Hypertrophy(Springer India, 2024) Apaydin, Emre; Yasar, Buse; Simsek, Gulcin; Kaygin, Pinar; Sarialtin, Sezen Yilmaz; Dirican, Onur; Cetin, Hazal EylemObjectiveAdenoid hypertrophy is a prevalent pediatric condition, often necessitating surgical intervention. Intranasal steroid administration shows promise as a conservative treatment, particularly by inducing apoptosis in adenoidal cells, leading to a reduction in adenoid size and inflammation. This study aims to characterize the expression profile of caspase-3 as an apoptotic inducer protein in inflammatory and epithelial adenoid tissues and explore its association with steroid administration. MethodsWe performed immunohistochemical staining for caspase-3 proteins in adenoid tissues obtained from 51 pediatric patients aged between 2.5 and 12 years (mean age: 6.09 +/- 2.1 years) who underwent adenoid surgery. A retrospective analysis of clinical data was conducted, categorizing participants into steroid treatment receivers (n = 25) and non-receivers (n = 26). Subsequently, the lymphoid inflammatory tissue and epithelial tissue from the adenoid were compared in terms of caspase-3 protein expression, and associated clinical variables were assessed. ResultsImmunohistochemical analysis revealed significant caspase-3 expression in inflammatory tissues. The expression levels were scored, and no significant correlation was observed between inflammation and epithelium based on caspase-3 expression (correlation coefficient = 0.143; p > 0.05). Furthermore, demographic and clinical characteristics did not show a statistically significant difference in caspase-3 expression levels. ConclusionCaspase-3 expression was significant in inflammatory adenoid tissue, but it showed no association with nasal steroid administration.Öğe The role of glutation-s-transferase mu1 and teta1 polymorphisms in chronic obstructive pulmonary disease(University of Ankara, 2021) Hayat, Buket; Yavuz, Muhsin Selçuk; Sahin, Engin; Dirican, Onur; Sarialtin, Sezen Yilmaz; Yilmaz, Can; Yildirim, IsilObjective: The aim of this study the investigation of "null" alleles in GSTM1 and GSTT1 gene regions in the development of COPD disease. Material and Method: There are 36 patients with COPD and 14 control cases, who received the Ethics Committee permission from Polatli Duatepe State Hospital Chest Diseases Department. DNA isolations were made from blood samples from the end of 2019 and the control group. Deletions in GSTM1 and GSTT1 gene regions were examined by qPCR method in COPD patient and control groups. The results of the study were evaluated comparatively by distributing the gene dose according to the Hardy-Weinberg. Result and Discussion: When seen from 36 COPD patients after qPCR analysis, it was found that deletion expressions due to gene doses in all patient groups; 23 individuals (63.8%) in the (+/-) genotype for GST-M1, 13 individuals (26.2%) in the (-/-) genotype. For GST-T1, 14 (%) in the (+/+) genotype 38.8), while deletion was observed with the highest rate, 4 individuals (11.1%) in the (+/-) genotype and 18 individuals (50.1%) in the (-/-) genotype were found. For GST-M1, deletion was observed in 19 individuals (63.3%) in the genotype (+/-) in male individuals, while it was observed in 4 individuals (66.6%) with the same genotype in women. While deletion was not observed in 11 (36.6%) male patients, this rate was observed as 2 (33.4%) in women. In the GST-T1 gene region, there were 10 (33.3%) males in male patients and 4 (66.6%) individuals in female patients with deletion occurring and the frequency of the "null allele" was high (+/+). In the (+/-) genotype, 3 (10%) in males and 1 (16.7%) in females were found. In the genotype where deletion was not observed and the gene was conserved (-/-), 17 (56.7%) individuals were observed in male patients and 1 (16.7%) in female patients. In the case that the gene "null" allele status in the GST-M1 gene region is slightly higher than the GST-T1 gene communication, this situation is thought to be a factor in obstructive pulmonary disease. © 2021 University of Ankara. All rights reserved.
