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    An enzyme free potentiometric detection of glucose based on a conducting polymer poly (3-aminophenyl boronic acid-co-3-octylthiophene)
    (Pergamon-Elsevier Science Ltd, 2013) Çiftçi, Hakan; Tamer, Uğur; Şen Teker, Mine; Pekmez, Nuran Özçiçek
    An enzyme free potentiometric glucose sensor was developed through the electrochemical preparation of a new poly (3-aminophenyl boronic acid-co-3-octylthiophene) (PAPBAOT) organic electrode. A PAPBAOT conducting polymer film electrode was first synthesized electrochemically on glassy carbon electrode and the analytical performance of the developed sensor was evaluated using potentiometric measurements. Boronic acid and the alkyl spacer functional group were used as molecular recognition and penetration agent, respectively. The PAPBAOT film electrode was characterized using cyclic voltammetry, Scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy, and Differential scanning calorimetry (DSC). The potentiometric calibration plot showed a hydrodynamic range of 5-50 mM glucose within a range of blood glucose level. The detection limit was 0.5 mM, and the sensor exhibited no interference from common interference such as ascorbic acid, dopamine, and uric acid. The PAPBAOT film electrode was used for the determination of glucose in human plasma samples and the results were compared with commercial amperometric sensors. (C) 2012 Elsevier Ltd. All rights reserved.
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    Paper membrane-based SERS platform for the determination of glucose in blood samples
    (Springer Heidelberg, 2015) Torul, Hilal; Çiftçi, Hakan; Çetin, Demet; Suludere, Zekiye; Boyacı, İsmail Hakkı; Tamer, Uğur
    In this report, we present a paper membrane-based surface-enhanced Raman scattering (SERS) platform for the determination of blood glucose level using a nitrocellulose membrane as substrate paper, and the microfluidic channel was simply constructed by wax-printing method. The rod-shaped gold nanorod particles were modified with 4-mercaptophenylboronic acid (4-MBA) and 1-decanethiol (1-DT) molecules and used as embedded SERS probe for paper-based microfluidics. The SERS measurement area was simply constructed by dropping gold nanoparticles on nitrocellulose membrane, and the blood sample was dropped on the membrane hydrophilic channel. While the blood cells and proteins were held on nitrocellulose membrane, glucose molecules were moved through the channel toward the SERS measurement area. Scanning electron microscopy (SEM) was used to confirm the effective separation of blood matrix, and total analysis is completed in 5 min. In SERS measurements, the intensity of the band at 1070 cm(-1) which is attributed to B-OH vibration decreased depending on the rise in glucose concentration in the blood sample. The glucose concentration was found to be 5.43 +/- 0.51 mM in the reference blood sample by using a calibration equation, and the certified value for glucose was 6.17 +/- 0.11 mM. The recovery of the glucose in the reference blood sample was about 88 %. According to these results, the developed paper-based microfluidic SERS platform has been found to be suitable for use for the detection of glucose in blood samples without any pretreatment procedure. We believe that paper-based microfluidic systems may provide a wide field of usage for paper-based applications.