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    A comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination
    (Springer, 2023) Büyük, Fatih; Karakaya, Emre; Akar, Mustafa; Kayman, Tuba; Tarhane, Serdal; Özcan, Hacer Ece; Çelebi, Özgür
    Helicobacter pylori is a pathogen associated with gastroduodenal diseases. This study aimed; (i) to investigate H. pylori presence by invasive tests in adult dyspeptic patients, (ii) to determine antibiotic susceptibility and genotypic characteristics of the H. pylori isolates, and (iii) to investigate the relationship between the H. pylori genotypes and the histopathological findings. In this cross-sectional study, gastric biopsy samples from 208 adult dyspeptic patients were used for culture, tissue Polymerase Chain Reaction (PCR), and histopathological analysis. Antibiotic susceptibility of the H. pylori isolates was analyzed by gradient method. Analysis of the virulence genes was performed by monoplex PCR. Genetic profiles (from A to H) were created based on the virulence genes presence. Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR) was used for the genotyping of the H. pylori isolates. The mean age of the patients was 46 (& PLUSMN; 15) years and 128 (61.5%) of them were female. H. pylori positivity was detected by culture, tissue PCR and histopathological examination in 59 (28.4%), 114 (54.8%) and 81 (38.9%) patients, respectively. The overall prevalence of H. pylori was found to be 63% (131/208). All H. pylori isolates were susceptible to tetracycline and amoxicillin. The resistance rates for metronidazole, clarithromycin, levofloxacin, and rifampicin were 67.2%, 27.9%, 34.4% and 13.11%, respectively. Multi drug resistance (MDR) was detected at the rate of 45.9% (28/61). While the most common virulence gene was cagA (93.44%), the least common was vacAm1 (23%). The predominant genetic profile was profile A (47.5%). ERIC-PCR results revealed a total of 26 different patterns. A high prevalence of H. pylori was detected in adult dyspeptic patients as in developing countries. It was observed significant genotypic heterogeneity and virulence gene diversity within the isolates. A considerable resistance rate detected against antibiotics such as clarithromycin, metronidazole, and levofloxacin, which are frequently used in the eradication of H. pylori, should be taken into consideration when creating regional empirical treatment regimens.
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    A comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination (vol 116, pg 1261, 2023)
    (Springer, 2023) Büyük, Fatih; Karakaya, Emre; Akar, Mustafa; Kayman, Tuba; Tarhane, Serdal; Özcan, Hacer Ece; Çelebi, Özgür
    [Abstract No tAvailable]
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    Correction: A comprehensive study of Helicobacter pylori infection: molecular analysis, antibacterial susceptibility, and histopathological examination (Antonie van Leeuwenhoek, (2023), 116, 12, (1261-1273), 10.1007/s10482-023-01868-3)
    (Springer Science and Business Media Deutschland GmbH, 2023) Buyuk, Fatih; Karakaya, Emre; Akar, Mustafa; Kayman, Tuba; Tarhane, Serdal; Ozcan, Hacer Ece; Celebi, Ozgur
    In the original publication of the article, the Fig. 1 text citation was incorrectly provided in the section “Virulence genes analysis”. However, it should have been Fig. 2. The Fig. 2 text citation was incorrectly provided in the section “Clinical data and diagnostic tests results”. However, it should have been Fig. 1. In the section “Antimicrobial testing results”, Fig. 2 text citation is removed. The figures 1 and 2 have been swapped correctly. The original article has been corrected. © 2023, Springer Nature Switzerland AG.
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    Helicobacter cappadocius sp. nov., from lizards: The first psychrotrophic Helicobacter species
    (Elsevier Gmbh, 2024) Aydin, Fuat; Tarhane, Serdal; Karakaya, Emre; Abay, Secil; Kayman, Tuba; Guran, Ozgur; Bozkurt, Emin
    It was aimed to determine the prevalence of Helicobacter in some reptilian and amphibian species in T & uuml;rkiye and to describe the bacteria. For this purpose, 73 cloacal swab samples were used as material. The description of the isolates was performed by detailed phenotypic tests, whole genome analyses, and MALDI-TOF MS. As a result of the phenotypic analysis, two helical, curved Gram-negative, motile isolates were recovered. It was determined through the analysis of 16S rRNA gene sequences that two isolates belonged to the genus Helicobacter. These isolates were found to be in a distinct group from other Helicobacter species. However, the 16S rRNA sequence did not match any identified species, with the closest match being Helicobacter mustelae strain R85-13-6T, which had an identity level of 96.2 %. Additionally, it was found that strains faydin-H75T and faydin-H76 had a 99.3 % identity level for their 16S rRNA genes. After conducting dDDH and ANI analyses, it was found that strains faydin-H75T and their close neighbors H.anseris ATCC BAA-1299T shared 13.5 % and 68.8 % similarity, respectively. The genome size of the strains was 1.7 Mb while G + C contents were 33.5 %. Metagenomic analyses using IMNGS and Protologger tools revealed the presence of faydin-H75T in various lizard species with high similarity, confirming its broad distribution and host specificity. The results indicated that these two strains represent a novel species, for which we propose the name Helicobacter cappadocius with faydin-H75T (=NCTC014972 = LMG 33382 = DSM117062) as the respective type strain. The current novel species is the first Helicobacter species to exhibit a psychrotrophic feature.