Yazar "Tonbak, Sukru" seçeneğine göre listele

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    Circulation of Schmallenberg virus in Turkey, 2013
    (Scientific Technical Research Council Turkey-Tubitak, 2016) Tonbak, Sukru; Azkur, Ahmet Kursat; Pestil, Zuleyha; Biyikli, Emel; Abayli, Hasan; Baydar, Ersoy; Bulut, Hakan
    Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1%) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98%-99% nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.
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    Complete genome analysis of highly pathogenic bovine ephemeral fever virus isolated in Turkey in 2012
    (Springer Wien, 2017) Abayli, Hasan; Tonbak, Sukru; Azkur, Ahmet Kursat; Bulut, Hakan
    Relatively high prevalence and mortality rates of bovine ephemeral fever (BEF) have been reported in recent epidemics in some countries, including Turkey, when compared with previous outbreaks. A limited number of complete genome sequences of BEF virus (BEFV) are available in the GenBank Database. In this study, the complete genome of highly pathogenic BEFV isolated during an outbreak in Turkey in 2012 was analyzed for genetic characterization. The complete genome of the Turkish BEFV isolate was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced. It was found that the complete genome of the Turkish BEFV isolate was 14,901 nt in length. The complete genome sequence obtained from the study showed 91-92% identity at nucleotide level to Australian (BB7721) and Chinese (Bovine/China/Henan1/2012) BEFV isolates. Phylogenetic analysis of the glycoprotein gene of the Turkish BEFV isolate also showed that Turkish isolates were closely related to Israeli isolates. Because of the limited number of complete BEFV genome sequences, the results from this study will be useful for understanding the global molecular epidemiology and geodynamics of BEF.
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    A Large-Scale Outbreak of Bovine Ephemeral Fever in Turkey, 2012
    (Japan Soc Vet Sci, 2013) Tonbak, Sukru; Berber, Engin; Yoruk, Mustafa Deniz; Azkur, Ahmet Kursat; Pestil, Zuleyha; Bulut, Hakan
    Regional cases of bovine ephemeral fever (BEF) were documented previously in Turkey. Previous cases were confirmed in South-East Turkey with low cow mortality. Recent BEF-suspected outbreaks with high mortality were documented in many regions of Turkey in 2012. The aim of study was the epidemiological examination of the outbreak and molecular characterization of the viruses detected from the outbreak. For this reason, blood samples were collected from BEF-suspected outbreak regions. From the results of RT-PCR, high rate of BEF-suspected samples (48/60 or 80%) was found positive for BEF virus (BEFV) RNA. The nucleotide sequences of the G(1) region of G gene of BEFV in the current study during the 2012 outbreak were grouped into cluster II of BEFV. It was suggested that BEFV may be spread out to other neighbor countries in the future years.