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    Alteration of tumor glucose metabolism after radiotherapy in MCF-7 breast cancer cell lines
    (UHOD - Uluslararasi Hematoloji Onkoloji Dergisi, 2006) Cengiz M.; Boyunağa H.; Yildiz F.; Ural A.U.; Atahan I.L.
    Cancer cells utilize anaerobic glycolytic way to compensate their faster metabolism when compared to normal cells. The purpose of this study is to investigate the effect of radiation on tumor metabolism. MCF-7 breast cancer cell lines were divided into 4 groups, including 2 control groups and aerobic and anaerobic study groups (were irradiated 600 cGy by Co-60 teletherapy unit), incubated with radiolabelled glucose for 4 hours. One control group was for aerobic, and the other was for anaerobic group after KCN addition. Radiolabelled CO2 produced by the cells was isolated and collected in specially designed simulation vials. In supernatant the measurements of other end-products of carbohydrate catabolism including lactate, pyruvate, acetate were performed on a liquid scintillation analyzer after they were collected via anion-exchange chromatography. Finally glucose in supernatant was measured enzymatically by glucose oxidase method. Glycogen consumption and lactate production were significantly higher in anaerobic and radiation groups (p<0.01). Whereas CO2 production was significantly higher in control group (p<0.01). Taken all results together radiation lead tumor cells more anaerobic glycolysis with high glycogen consumption, high lactate production and low CO2 production. Radiation itself has led tumor cells to produce energy by anaerobic glycolysis, meaning radiation exposed cells become more hypoxic.
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    Öğe
    Anaerobic glycolysis is the main pathway for energy generation in HI-60 acute promyelocytic leukemia cells
    (Derman Medical Publishing, 2010) Boyunaga H.; Keles H.; Kenar L.; Ural A.U.; Avcu F.
    Aim In physiological conditions, normal cells use mainly the glycolytic aerobic pathway to provide energy. However, most cancer cells utilize anaerobic glycolytic way for energy generation. Aim of this study was to investigate the carbohydrate metabolic pathways of HL-60 acute promyelocytic leukemia cells for energy production. Material and Methods Leukemia cells as well as normal leukocytes were incubated with radiolabelled glucose in aerobic and anaerobic conditions and glycogen consumptions and the ratios of radiolabelled glucose catabolized into CO2 or lactate, that is, the rates of aerobic or anaerobic glycolysis, were determined. Results The glycogen consumption was significantly higher in aerobic leukemia cell culture than normal leukocyte culture (p<0.01). The rate of anaerobic glycolysis was 93.8% in leukemia cells in aerobic conditions and it increased to 96.6% while utilization of glycogen increased by 7.31% in anaerobic conditions. Conclusion In conclusion, principally anaerobic glycolysis is effective for energy generation in HL-60 promyelocytic leukemia cells. This result may be important for the development of new therapeutic approaches in the treatment of promyelocytic leukemia, requiring further comprehensive studies.