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    CD14 and CD44s Expression in Patients with Non-Small Cell Lung Cancer
    (Ortadogu Ad Pres & Publ Co, 2011) Atinkaya, Cansel; Bilgic, Emre; Yazici, Ulku; Taspinar, Mehmet; Oz, Gurhan; Yuruker, Sinan; Tastepe, Abdullah Irfan
    Objective: Lung cancer is one of the most common cancers with high mortality. Only 10-15% of the patients with lung cancer survive more than five years despite advanced treatment strategies. Features of tumor immunity are important in carcinogenesis, and immunological mechanisms must be clarified. The role of CD14 and CD44s proteins in tumor immunity of lung cancer is controversial, and studies on these proteins mostly were held on cancer cell lines. In this study, we aimed to investigate CD14 and CD44s protein expressions in tumor and normal tissues in patients with non-small cell lung cancer. Material and Methods: Thirty patients (25 males and 5 females) with non-small cell lung cancer were included in this study. Specimens obtained during the surgery were frozen in liquid nitrogen, and sliced with a thickness of 5 pm using a microtome. Standard immunohistochemical procedures were used for staining and visualization. Differences in staining patterns between normal and tumor tissues were analyzed statistically with Chi-square test.Results: CD14 and CD44s protein expressions were found both in tumor and in the normal tissues. There was no statistically significant difference in staining patterns between normal and tumor tissues (p>0.05).Conclusion: The expression of the two molecules in both tumoral and healthy tissues demonstrates that different pathways of tumor immunity affect the prognosis of the patients. However, further studies are needed in different types of cancers with larger numbers of cases and different antibodies are needed to clarify the role of CD14 and CD44s.
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    The effect of CYP1A1, GSTT1 and GSTM1 polymorphisms on the risk of lung cancer: A case-control study
    (Sage Publications Ltd, 2012) Atinkaya, Cansel; Taspinar, Mehmet; Sakiragaoglu, Onur; Oz, Gurhan; Yazici, Ulku; Oztuna, Derya; Sunguroglu, Asuman
    Lung cancer, which is mainly affected by environmental factors, is a lethal malignancy. It is also important to investigate the effect of genetic factors on lung cancer aetiology. In this study, we aimed to investigate the distribution of CYP1A1*2C, GSTT1 and GSTM1 polymorphisms in Turkish lung cancer patients to determine whether any promoting effect of polymorphisms could cause development of lung cancer. For this purpose, genomic DNA samples obtained from peripheral blood of 128 patients with lung cancer and 122 healthy subjects were analyzed. Genotyping of polymorphic enzymes were carried out by polymerase chain reaction-restriction fragment length polymorphism methods. Although there were no significant differences between groups in terms of CYP1A1 polymorphism, the carriers of CYP1A1 Ile/Val genotype (odds ratio [OR] = 1.224, 95% confidence interval [CI]: 0.585-2.564) or CYP1A1 Val/Val genotype (OR = 3.058, 95% CI: 0.312-30.303) had an increased risk of lung cancer development. There was no statistical difference between groups in terms of both GSTT1 null genotype (OR = 1.114, 95% CI: 0.590-2.105) and GSTM1 null genotype (OR = 0.776, 95% CI: 0.466-1.290). This is the first case-control study investigating CYP1A1 Ile/Val, GSTT1 and GSTM1 polymorphisms in Turkish lung cancer patients. Although we suggest that other genes in addition to the proposed genes could play a role in lung cancer development, the results of our study will contribute to the possible associations between CYP1A1 Ile/Val, GSTT1 and GSTM1 gene polymorphism on the risk of lung cancer.
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    The expression of GST isoenzymes and p53 in non-small cell lung cancer
    (Via Medica, 2010) Oguztuzun, Serpil; Aydin, Mehtap; Demirag, Funda; Yazici, Ulku; Ozhavzali, Muzeyyen; Kilic, Murat; Iscan, Mesude
    This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase alpha, pi, mu, theta and p53 in non-small cell lung carcinoma and normal lung tissue from 50 patients. The relationships between expressions of the Glutathione-S-transferase isoenzymes and some clinicopathological features were also examined. Expression of glutathione-S-transferase pi, mu, alpha, theta and p53 was assessed by immunohistochemistry for primary lung carcinomas of 50 patients from the Sanitarium Education and Research Hospital, Ankara lung cancer collection. The relationships between expression of the glutathione-S-transferase isoenzymes, p53 in normal and tumor tissue by Student T test and the clinicopathological data were also examined by Spearman Rank tests. When the normal and tumor tissue of these cases were compared according to their staining intensity and percentage of positive staining, glutathione-S-transferase alpha, pi, mu, theta expressions in tumor cells was significantly higher than normal cells (p<0.05). There was no significant difference in the expression of p53 between normal and tumor cells (p>0.05). When the immunohistochemical results of glutathione-S-transferase isoenzymes and p53 were correlated with the clinical parameters, there were no significant associations between glutathione-S-transferases and p53 expressions and tumor stage, tumor grade and smoking status (p>0.05).