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Öğe Antimicrobial activity of endemic Digitalis lamarckii Ivan from Turkey(Natl Inst Science Communication-Niscair, 2009) Benli, Mehlika; Yiğit, Nazife; Geven, Fatmagül; Güney, Kerim; Bingöl, ÜmitAntimicrobial activity of the methanolic extracts of leaves and flowers of D. lamarckii Ivan, (Scophulariaceae), an endemic plant species of Turkey, was tested on ten bacterial and four yeast strains. Effective antibacterial activity was observed in four bacterial strains. Minimum inhibitory concentration (MIC) was calculated by use of liquid culture tests and in all the four effective bacterial strains, the MIC was found to be >= 199.5 mg/ml. The minimum bactericidal concentration (MBC) of B. subtilis, S. aureus, and L. monocytogenes was calculated to be >= 199.5 mg/ml, and MBC value for Shigella was calculated as >= 399 mg/ml.Öğe Functional morphology of the venom apparatus of Larinioides ixobolus (Araneae: Araneidae)(2006) Yiğit, Nazife; Bayram, Abdullah; Danışman, Tarık; Sancak, ZaferLarinioides ixobolus (Thorell, 1873) is widely distributed throughout Middle Europe, Turkey and Middle Asia. The morphology of the venom apparatus of L. ixobolus using adult spiders that were collected from Kirikkale (Turkey) were investigated by scanning electron microscopy. The general organization of the venom apparatus of L. ixobolus is similar to other spiders' venom apparatus. The venom apparatus, situated in the anterior of the prosoma, is composed of a pair of chelicerae and venom glands. Each chelicera consists of a stout basal and a movable apical (fang) segments. The fang rests in a groove on the basal segment. Both sides of the cheliceral grooves are armed with three marginal teeth. To eject the venom, a venom pore is situated on the subterminal part of the fang. The venom glands of L. ixobolus are equal size and they look like a carrot. Each gland is surrounded by bulky muscular layer. Also, the nerve cells can be easily observed on the surface on the venom gland by Scanning Electron Microscope (SEM). © 2006 Asian Network for Scientific Information.Öğe Functional structure of Agelena labyrinthica's (Araneae : Agelenidae) venom gland and electrophoresis of venom(Pergamon-Elsevier Science Ltd, 2006) Yiğit, Nazife; Güven, TuranThe funnel-web spider, Agelena labyrinthica, is widely distributed throughout Turkey. The objective of the present study was to describe the histological and functional fine structure of A. labyrinthica's venom gland by using light microscope, scanning (SEM) and transmission electron microscope (TEM). We have also preliminarily analyzed venom components by SDS-PAGE. Each venom gland has surrounded by a thin adventitia and gross striated muscular bundles. Basal lamina underlies between muscular bundles and the inner glandular epithelium, and ties up them each other. The striated muscular bundles spirally covered venom gland has been observed by SEM. Intricate relations formed between motor neuron axons and the muscle fibers have been revealed by TEM. The secretory epithelium, which made up of simple columnar cells, formed the secretory region of the venom gland. The secretory surface of the gland was increased by a sort of fringes extended from basal membrane into the gland lumen. The epithelial cells have many rough endoplasmic reticulum, mitochondria and different size and shape of secretory granules. These granules have been accumulated in apical portion of the secretory cells. After the gland is emptied, the apical portions of secretory cells deteriorate and the basal epithelial cells regenerate the columnar cells. The analysis of A. labyrinthica venom, which was achieved by SDS-PAGE showed that there have been at least seven components ranging from 10 to 40 kDa molecular weight. (C) 2005 Elsevier Ltd. All rights reserved.Öğe Loxosceles: A case healed completely without any necrotic tissue by emergency department and review of the literature(2013) Gülalp, Betül; Emre, Afşin Kayıpmaz; Altınörs, Mehmet Nur; Sancak, Zafer; Yiğit, NazifeLoxosceles ısırığında görülen en sık bulgu ısırık yerinde progresif ülsere dermonekrozdur. Sphingomyelinase D zehirdeki asıl dermonekrotik faktördür. Zehir küçük kapillerlerde doku nekrozu başlangıcı olan hızlı koagülasyon ve oklüzyona neden olarak etkisini gösterir. 28 yaşında kadın hasta, sabah ayakkabısını giymeye çalışırken kahverengi bir örümcek tarafından ısırılmasından 3-4 saat sonra kliniğimize başvurdu. Sağ ikinci parmak iç proksimalinde lokal minimal inflamasyondan başka bulgusu yoktu. Ertesi gün gelen hastada ayak bileğine kadar orta derecede ödem, ısırık yerinden başlayarak proksimale ilerleyen sınırları net olmayan palpasyonla ısı artışı ve geniş kırmızı-mor cilt lezyonu mevcuttu. Hospitalizasyonun ilk günü CBCde RDW 15,50, lökosit 11,20, nötrofil %79,80, lenfosit %11,30, CRP 16, ABKda CPK 455, LDH 78, se- dimantasyon, PT, PTZ, kan gazı parametreleri normal sınırlarda idi. Ayak düz filmi çekildi. Ayak stabilizasyon ve elevasyonu sağlandı. Loxosceles zehirlenmesi öntanısıyla Acil Tıp adına 5 gün hospitalize edildi. Hospitalizasyonda sıvı replasmanı, antibiyotik, kortikosteroid, antiinflamatuar ile konservatif tedavi başlandı. Dördüncü hafta hafif pigmente cilt bulgusu dışında doku nekrozuna ait komplikasyon gelişmeksizin iyileşme sağlandı. Böcek sokmaları ile ilk anlarda belirgin bir şikayet ve bulgu olmaması klinisyeni yanıltmamalıdır. Bu hastaların izlemi ve kontrole çağrılmasının ciddi komplikasyonlar ve atlanabi- lecek olgularda önemi açıktır. (JAEM 2013; 12: 101-4)Öğe New records for the turkish araneo-fauna: theridion varians hahn, 1833, dipoena melanogaster (c. l. koch, 1837) and achaearanea riparia (blackwall, 1834) (araneae: theridiidae)(2007) Bayram, Abdullah; Danışman, Tarık; Yiğit, Nazife; Çorak, İlkay; Sancak, Zafer[No abstract available]
