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Öğe Effects of cholesterol and cAMP on progesterone production in cultured luteal cells isolated from pseudopregnant cat ovaries(Elsevier Science Bv, 2009) Arikan, Sevket; Yigit, Ayse ArzuThe present study was designed to incubate luteal cells isolated from pseudopregnant cats and to investigate the effects of cholesterol and cAMP on luteal progesterone production. Corpora lutea were collected from the cats on days 10 and 15 of pseudopregnancy. Luteal cells were isolated from the ovaries by collagenase digestion. Steroidogenic luteal cells were stained for 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) activity. Cells (2 x 10(4)) staining positive for 3 beta-HSD were cultured for up to 7 days. The cells were treated with 22(R)-hydroxycholesterol (22R-HC) and dibutyryl cyclic AMP (dbcAMP) on days 1, 3 and 7. Treatment of cells with 22R-HC resulted in a dose-dependent increase (p < 0.001) in progesterone production. When 22R-HC was used at a concentration of 10 mu g/ml, it resulted in 2.7- and 5.1-fold increases in progesterone production on days 3 and 5, respectively. When the dose was doubled (20 mu g/ml), treated cells produced four times more progesterone on days 3 and 7, and three times more on day 5. By day 7, progesterone production increased up to 9.1 times more than the control. Incubation of cells with both concentrations of dbcAMP (0.1 mM and 1 mM) resulted in significant stimulations of progesterone on days 5 and 7 (p < 0.001). However, on day 3, only higher doses of dbcAMP (1 mM) resulted in significant stimulation (p < 0.05). Progesterone production was increased up to 2- and 2.9-fold of the control when cells were treated with lower concentration of dbcAMP (0.1 mM) on days 5 and 7. respectively. Incubation of cells with 1 mM concentrations of dbcAMP induced a 3.2-fold increase on day 5 and a 5-fold increase on day 7. In conclusion, a successful incubation was performed for long-life culturing of luteal cells collected from pseudopregnant cats. The method works well and allows for optimal growth and development of cells in the culture. The present study also demonstrated that incubating cat luteal cells with 22R-HC and dbcAMP induces a significant increase in luteal progesterone synthesis. (C) 2008 Elsevier B.V. All rights reserved.Öğe Effects of incubator oxygen and carbon dioxide concentrations on hatchability of fertile eggs, some blood parameters, and histopathological changes of broilers with different parental stock ages in high altitude(Elsevier, 2022) Okur, Nezih; Eratalar, Sabri Arda; Yigit, Ayse Arzu; Kutlu, Tuncer; Kabakci, Ruhi; Ozsoy, Sule YurdagulThe effects of incubator carbon dioxide (CO2) and oxygen (O-2) concentrations with parental stock age (PSA) on embryonic deaths (ED), hatchability of fertile eggs (HFE), some blood parameters, and the tissue development of broilers were investigated. Four consecutive repetitions following the similar materials and methods were carried. From 3 different aged ROSS 308 broiler parental flocks 7,680 hatching eggs were obtained and classified as young (Y; 29 wk), middle (M; 37 wk) and old (O; 55 wk) as regards PSA, and randomly distributed. Four different incubator ventilation programs (IVP) as control (C; 0.67% CO2 and 20.33% O-2), high CO2 (HC; 1.57% CO2 and 20.26% O-2), high O-2 (HO; 0.50% CO2 and 21.16% O-2), and high CO2 + O-2 (HCO; 1.17% CO2 21.03% O-2) were applied with oxygen concentrator, and ED and HFE were investigated. Lung and heart tissues, hemoglobin value, packed cell volume, and red blood cell count, triiodothyronine, thyroxine, adrenocorticotropic hormone (ACTH) values of the chicks were analyzed. It was found that IVP affected ED and HFE. Higher rate of early ED (EED) was obtained from the HC than HCO, and higher middle+late stage +pipped but unhatched ED (MLPED) with a lower rate of HFE was observed in the C group than HO and HCO (P < 0.05). Association was found between PSA and IVP (P < 0.05), being more evident in EED for young PSA, in MLPED with HFE for Y and O PSA. From hematological values, no statistical difference in RBC, PCV, and Hb values were found among the treatment groups, ACTH concentration known as a response to stress was found to be higher than C in all groups, triiodothyronine concentration was higher in the HO group than C. In the histopathological examination, used IVPs were found to have negative effects on the lung and heart such as vacuolization, hemorrhage in all PSA groups except for C. Conclusively, PSA and IVP affected some hatching, blood and tissue development parameters of the broiler chicks.Öğe Evaluation of the Pre-Hemolytic Concentrations of β-, Methyl-β- and Dimethyl-β-Cyclodextrin on Dog and Goat Erythrocytes(Medwell Online, 2008) Yigit, Ayse Arzu; Arikan, Sevketbeta-Cyclodextrin (beta-CD) is a cage-like molecule consisting of seven glucose units and have appropriate size to form inclusion complexes with many hormones, vitamins and drugs. Due to having very poor aqueous solubility, beta-cyclodextrin is modified chemically for various applications. Experiments were conducted to examine effects of pre-hemolytic concentration of beta-cyclodextrin and its modified derivatives, methyl-beta-cyclodextrin (M beta-CD) and dimethyl-beta-cyclodextrin (DM beta-CD), on the osmotic fragility of dog and goat erythrocytes. Blood samples collected from healthy 10 dogs and 10 goats were analyzed within 5 h of collection. Erythrocyte suspensions were mixed with various concentrations of beta-CD, M beta-CD and DM beta-CD. The mixtures were then incubated for 30 min at 37 degrees C and osmotic fragility of erythrocytes was measured. Incubation of all 3 Cyclo Dextrins (CDs) with erythrocyte suspensions of both species, dog and goat, induced a dose dependent increase in the erythrocyte osmotic fragility (p<0.01). The hemolytic effect was decreased in order DM beta-CD>M beta-CD>beta-CD. Hemolytic doses of these CDs were lower for goat erythrocytes than those of the dog erythrocytes. When beta-CD and its methylated derivatives are even used in pre-hemolytic concentrations, they might also reduce life span of erythrocytes. Due to importance of scientific knowledge on the cellular activity of cyclodextrins and growing number of its potential applications on diagnosis food preparations, our results may help to deal with the hemolytic activity of CDs for in vivo studies.Öğe Influence of Florfenicol on Hematological Parameters in Broilers(Wiley, 2018) Yigit, Ayse Arzu; Yildirim, Ebru; Baydan, Emine; Aydogan, Ilkay; Ekici, Husamettin; Guncum, Enes…Öğe The Influence of Pentabrominated Diphenylether 99 on Progesterone Secretion by Bovine Luteal Cells(Wiley, 2017) Kabakci, Ruhi; Yigit, Ayse Arzu…Öğe Investigation of the Achievability of Stem Cells Derived from Luteal Cells in Rats(Wiley, 2018) Yigit, Ayse Arzu; Orscelik, Emine Umran; Boyuk, Gulbahar; Tiryaki, Meral; Pinarli, Ferda Alparslan…Öğe Investigation of the Effects of Metribuzine on Cow Granulosa Cell Steroidogenesis(Wiley, 2022) Yigit, Ayse Arzu; Kahveci, Metehan Eser; Kabakci, Ruhi[Abstract No tAvailable]Öğe Malondialdehyde and nitric oxide levels and catalase, superoxide dismutase, and glutathione peroxidase levels in maternal blood during different trimesters of pregnancy and in the cord blood of newborns(Tubitak Scientific & Technical Research Council Turkey, 2015) Yuksel, Sevda; Yigit, Ayse ArzuBackground/aim: To determine whether levels of malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) change during the 3 trimesters of pregnancy, and to compare the third trimester of pregnancy with newborn cord blood in respect to the levels of MDA, NO, and antioxidant enzymes. Materials and methods: Maternal blood samples were collected at 9-13, 22-26, and 36-40 weeks of gestation. Cord blood was collected at the time of delivery. Results: NO levels and GPx activity were higher in the second and third trimesters than in the first trimester. MDA level was lower in the third trimester and SOD and CAT activities were lower in the second and third trimesters than the first trimester. In cord blood, MDA and NO levels were lower while CAT and GPx activities were higher than in the third trimester of pregnancy. Conclusion: These results suggest that the balance of free radical and antioxidant production during pregnancy ensures healthy fetus growth and development.Öğe An Optimization of Long Term Culture of Rat Islet Cells(Wiley-Blackwell, 2015) Yigit, Ayse Arzu; Boyuk, Gulbahar; Delibasi, Tuncay…Öğe Oxidant and antioxidant status of human breast milk during lactation period(Springer France, 2015) Yuksel, Sevda; Yigit, Ayse Arzu; Cinar, Miyase; Atmaca, Nurgul; Onaran, YukselDue to the oxidative stress conditions at birth, newborn needs some immunological and antioxidative protection after birth. The present study was conducted to point out the oxidant and antioxidant activity of human breast milk throughout the lactation. For this purpose, the levels of malondialdehyde, nitric oxide, lactoferrin, superoxide dismutase, and catalase was determined in colostrum, transition milk, and mature milk. Milk samples were collected from 20 volunteer mothers at 24-48 h of delivery and 7 and 14 days after delivery. The skimmed milk was used for determination of the levels of malondialdehyde, nitric oxide, and lactoferrin and the activities of the catalase and superoxide dismutase enzymes. As the lactation period proceeded, the malondialdehyde level increased and the levels of nitric oxide and lactoferrin as well as the activities of catalase and superoxide dismutase decreased. Colostrum has a high antioxidant capacity, and these antioxidants decrease during the lactation period owing to the changing need of the growing infant. The results of this study revealed the importance of breastfeeding immediately after the birth to take the advantage of colostrum.
