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Öğe Contribution of Bone Marrowe-Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats(Academic Press Inc Elsevier Science, 2021) Demir, Sabri; Erturk, Ahmet; Gunal, Yasemin Dere; Ozmen, Ismail; Zengin, Mehmet; Yildiz, Dincer; Karaoz, ErdalBackground: The most common thoracic injury in children, resulting in trauma, is pulmonary contusion (PC). Bone marrowe-derived mesenchymal stem cells (BM-MSCs) are used in wound healing and many other diseases. This study aims to examine the effects of BM-MSCs on PC healing in rats. Materials and methods: A total of 45 male Wistar albino rats were used. Four groups were formed. BM-MSCs were labeled with the green fluorescent protein. PC was observed in the control group. In group II, PC occured and left to spontaneous healing. In group III, PC formed and BM-MSCs were given. In group IV, BM-MSCs were given without PC formation. Subjects were sacrificed 1 week later. Whether there was any difference in terms of BM-MSC involvement and lung injury score was investigated. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS), version 17.0, software (SPSS Inc., Chicago, IL), and p value of <0.05 was considered statistically significant. Results: BM-MSCs were collected much more in the lungs in group III than in group IV. Group III had a lower lung injury score value than group II. Conclusion: The greater involvement of the BM-MSCs in the injury site, and further reductions in lung injury score suggest that BM-MSCs are contributing to the healing of the injury. The use of BM-MSCs in risky patients with diffuse PC may be an alternative treatment to conventional methods. (C) 2020 Elsevier Inc. All rights reserved.Öğe Contribution of Bone Marrow–Derived Mesenchymal Stem Cells to Healing of Pulmonary Contusion-Created Rats(Academic Press Inc., 2021) Demir, Sabri; Erturk, Ahmet; Gunal, Yasemin Dere; Ozmen, Ismail; Zengin, Mehmet; Yildiz, Dincer; Karaoz, ErdalBackground: The most common thoracic injury in children, resulting in trauma, is pulmonary contusion (PC). Bone marrow–derived mesenchymal stem cells (BM-MSCs) are used in wound healing and many other diseases. This study aims to examine the effects of BM-MSCs on PC healing in rats. Materials and methods: A total of 45 male Wistar albino rats were used. Four groups were formed. BM-MSCs were labeled with the green fluorescent protein. PC was observed in the control group. In group II, PC occured and left to spontaneous healing. In group III, PC formed and BM-MSCs were given. In group IV, BM-MSCs were given without PC formation. Subjects were sacrificed 1 week later. Whether there was any difference in terms of BM-MSC involvement and lung injury score was investigated. Statistical analysis was performed using the Statistical Package for Social Sciences (SPSS), version 17.0, software (SPSS Inc., Chicago, IL), and p value of <0.05 was considered statistically significant. Results: BM-MSCs were collected much more in the lungs in group III than in group IV. Group III had a lower lung injury score value than group II. Conclusion: The greater involvement of the BM-MSCs in the injury site, and further reductions in lung injury score suggest that BM-MSCs are contributing to the healing of the injury. The use of BM-MSCs in risky patients with diffuse PC may be an alternative treatment to conventional methods. © 2020 Elsevier Inc.Öğe Determination of Goose Feather Morphology by Using SEM(Medwell Online, 2009) Yildiz, Dincer; Bozkurt, Emine Umran; Akturks, S. HakanGoose feathers have importance because of not only a widespread usage in consequence of its lighter weight, softness and more compressible than other similar insulation materials, but also its highly organized dermoidal structure. Feather samples are taken from thoracal, cervical, dorsal, ventral wing and dorsal wing regions of local geese in both sexes. Samples covered with chrome by Polaron SC 500 and are examined by JSM 7000 Scanning Electron Microscope (SEM). Plumes have peculiarities in entrapping the air. Barbs in breast feathers arise bilaterally from rachis at about 35 degrees C. There is an increasing number of hooklets on the barbuls from proximal to distal end. The barbuls, which arise from contiguous barbs cross each other at 90 degrees C. Contour feathers on breast region are shorter than other general contour feathers in others areas. The most notable differences between cervical feathers are that they are 4 times longer in male than female and diameter of scapus is bigger in male. It represents that there is intensive vexilla in female because of their barbs which are thinner than those in male. The most important reason of softness and puffiness of plumae and semiplumae is that barbs are not connected each other in spite of they have hamulus. It is proposed that SEM can be very useful toot for textile industry to determine quality of the goose feathers.Öğe The first report of Eucoleus dispar (Nematoda: Capillariidae) in the common buzzard (Buteo buteo) in Turkey(Scientific Technical Research Council Turkey-Tubitak, 2015) Yildiz, Dincer; Buyukcopur, Emine Umran Bozkurt; Gultiken, Murat Erdem; Yildiz, KaderA common buzzard (Buteo buteo) was brought to the Department of Anatomy, Kirikkale University Faculty of Veterinary Medicine, following its death from trauma. The tongue of the buzzard was processed for routine histology and stained with hematoxylin and eosin. A curled Eucoleus dispar and its eggs, embedded in the dorsal epithelium of the tongue, were observed in histological sections during morphological analysis of the papillae lingua in the buzzard under a light microscope. According to the authors' knowledge, this is the first report on E. dispar in the buzzard in Turkey.Öğe Morphological examination of the bulbus oculi and intraorbital muscles (musculi bulbi) in the common buzzard (buteo buteo)(Ankara Univ Press, 2011) Gultiken, Murat Erdem; Onuk, Burcu; Yildiz, Dincer; Yilmazer, BesteIn this study, a total of 20 eyeballs belong to 10 adult buzzards (buteo buteo) were examined. Morphometric data of eyeballs and lens were described and value of right and left eyes were evaluated with "paired sample t-test" and significant difference was not found (p >= 0.05). Bony plates described as scleral ossicles were observed in equator region of sclera in histological investigation. Sinusoidal cavities were noticed in bony plates. A cartilage containing chondrocytes and chondroblasts in it in lower part of these bony plates were seen. Intraorbital muscles, dorsal rectus, ventral rectus, nasal rectus, temporal News, dorsal oblique and ventral oblique were responsible for the movement of bulbus oculi. Also, pyramidalis and quadrants muscles that originated from bulbus oculi and attached to nictitating membrane were determined. It was detected that rectus muscles and ventral oblique muscle originated from the surrounding of optic foramen and dorsal oblique muscle originated from nasal part of interorbital septum and attached to sclera. Pyramidalis and quadrants muscles were stated to be attached to sclera and to responsible for the movement of nictitating membrane. Morphometric data and junction formation of the insertion tendon of intraorbital muscle to the sclera were identified.Öğe The Morphology of the Os Penis in the Adult Mouse(Medwell Online, 2010) Yildiz, Dincer; Bolat, Durmus; Karahan, SiyamiThis study focused on morphology of the os penis in adult mouse (Mus musculus domesticus). The os penis was located within the corpus cavernosum penis and extended up to the half way of the glans penis. With a close resemblance to that of the rat os penis, the shape of the mouse os penis appeared a probe-like structure. Histologically, the body of os penis was consisted of compact bone and a narrow bone marrow. Hyaline cartilage constituted the proximal end, to which the corpus cavernosum penis attached and blended with the perichondrium. A completely ossified proximal end was rarely observed. The organization of hyaline cartilage at the proximal end resembled the growth plate of the long bone. However, chondrocytes did were not well organized into columns as in the growth plate. Like in the physis of the long bone, invasion of hyperthropic chondrocyte by blood vessels originating from the underlying bone was present; however, chondrocytes embedded directly in osteoid matrix were also quite common. While the proximal end of the os penis was covered with hyaline cartilage, the distal bony end was continuous with a type of tissue varied from loose to dense connective tissue and to fibrocartilage-like tissue. At the distal bony end, newly synthesized osteoid matrix was distinguishable. Thus, the os penis in the adult mouse is a dynamic structure exhibiting continuous growth both at the proximal and distal ends.Öğe Ultrastructure of the tongue and histochemical features of the lingual salivary glands in buzzards(Scientific Technical Research Council Turkey-Tubitak, 2018) Bozkurt, Emine Umran; Gultiken, Murat Erdem; Yildiz, Dincer; Bolat, DurmusThe macroscopic characteristics of the tongues of 8 long-legged buzzards are described and scanning electron microscopy was used to examine the epithelial tissue of the tongue's surface. Crossman's modified triple staining method and hematoxylin and eosin dye were used to determine the locations and general histological features of the lingual salivary glands. The nature of the glandular secretions was examined by staining with PAS, AB pH 1.0, AB pH 2.5, and PAS-AB pH 1.0 and pH 2.5, and diastase enzyme digestion. PAS staining and weak, moderate, and strong methylation procedures as well as sialidase and hyaluronidase applications were performed. We report the finding of focal accumulations of the anterior lingual salivary glands at the tongue's base with numerous duct openings in this area. This finding, in conjunction with the absence of taste papillae in the tongue epithelium, suggests that the role of the tongue in buzzards is to mix food with saliva and to move the food bolus rapidly to the back of the oral cavity. The acidic secretions of the lingual salivary glands not only have antimicrobial effects but also facilitate the swallowing process and may influence the digestive process in the distal parts of the alimentary tract.
