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    Histopathological, Immunohistochemical and Molecular Detection of Toxoplasma gondii in Organs and Tissues of Experimentally Infected Mice
    (Hellenic Veterinary Medical Soc, 2024) Yucesan, B.; Kilic, S.; Alcigir, M. E.; Babur, C.; Ozkan, O.
    With Toxoplasma gondii mouse experiments, it is aimed to determine its distribution to organs and tissues histopathologically, immunohistochemically, and molecularly. In our study, T. gondii _TR01 tachyzoites were injected intraperitoneally into the mice. The parasite was determined in daily concentrations in blood, peritoneal fluid, liver, kidney, heart, lung, intestine, and central nervous system sections. Mild necrosis and degeneration of hepatocytes and degeneration of cortical and corticomedullary tubular epithelium in construction were observed as a result of histopathological examinations. Mild degeneration was observed in the heart. Regional hyperemic capillaries were found in the lung. Degeneration was observed in intestinal epithelial cells, and no necrosis was observed in villi and glandular epithelium. There was degeneration and necrosis of cerebral cortical neurons in the CNS. In immunohistochemical staining, reactions were mostly found in the liver, kidney, and intestine, while relatively low levels were found in the lung, heart, and brain. RT-PCR targeting the T. gondii B1 gene region molecularly was used. On the first and last days, it is mostly found in the liver, lung, and peritoneal fluid. At least it spread to the brain and heart. Tissue cysts were not found in all tissues. The spread to the heart is low. The liver, kidneys, and peritoneal fluid are most affected, and the brain is the least affected. Necrosis was detected in all tissues except the intestine. Organ and tissue spreads should be determined for drug and vaccine studies of T gondii. This study will assist in meeting this demand.
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    Investigation of Microbiological, Physiological and Histopathological Changes in Polymicrobial I nfection in Old Rats
    (Agricultural Research Communication Centre, 2024) Ulker, U.; Kizil, S.; Bayraktar, B.; Alcigir, M. E.; Kul, O.; Kaya, H.; Urusan, H.
    Background: The aim of this study is to examine the microbiological, clinical, physiological and pathological findings of natural polymicrobial infection developing in a wistar rat colony under long-term observation. Methods: In this study, 70 male Wistar albino rats, 3 years old, raised by conventional methods and housed in a cage environment were used. Thirteen rats with clinical symptoms such as torticollis and rotation were sacrificed for microbiological and histopathological examination after 14 days. For microbiological examination, the rapid diagnostic kit BBL Crystal system was used to identify bacterial agents in tissue and stool samples and the traditional culture method was used for isolation. Result: As a result of microbiological analysis, the rarely isolated Staphylococcus capitis ( S. capitis ) was isolated. Different Staphylococcus spp. It has been determined that the species can cause suppurative meningoencephalitis as well as septicemia in the brains of experimental animals., it is thought that polymicrobial infection will be useful for the identification of bacterial agents and infection prevention and treatment strategies.