Histopathological, Immunohistochemical and Molecular Detection of Toxoplasma gondii in Organs and Tissues of Experimentally Infected Mice
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Date
2024
Journal Title
Journal ISSN
Volume Title
Publisher
Hellenic Veterinary Medical Soc
Access Rights
info:eu-repo/semantics/openAccess
Abstract
With Toxoplasma gondii mouse experiments, it is aimed to determine its distribution to organs and tissues histopathologically, immunohistochemically, and molecularly. In our study, T. gondii _TR01 tachyzoites were injected intraperitoneally into the mice. The parasite was determined in daily concentrations in blood, peritoneal fluid, liver, kidney, heart, lung, intestine, and central nervous system sections. Mild necrosis and degeneration of hepatocytes and degeneration of cortical and corticomedullary tubular epithelium in construction were observed as a result of histopathological examinations. Mild degeneration was observed in the heart. Regional hyperemic capillaries were found in the lung. Degeneration was observed in intestinal epithelial cells, and no necrosis was observed in villi and glandular epithelium. There was degeneration and necrosis of cerebral cortical neurons in the CNS. In immunohistochemical staining, reactions were mostly found in the liver, kidney, and intestine, while relatively low levels were found in the lung, heart, and brain. RT-PCR targeting the T. gondii B1 gene region molecularly was used. On the first and last days, it is mostly found in the liver, lung, and peritoneal fluid. At least it spread to the brain and heart. Tissue cysts were not found in all tissues. The spread to the heart is low. The liver, kidneys, and peritoneal fluid are most affected, and the brain is the least affected. Necrosis was detected in all tissues except the intestine. Organ and tissue spreads should be determined for drug and vaccine studies of T gondii. This study will assist in meeting this demand.
Description
Keywords
Histopathology; Immunohistochemical; PCR; Tissues; Toxoplasma gondii
Journal or Series
Journal of The Hellenic Veterinary Medical Society
WoS Q Value
N/A
Scopus Q Value
Q4
Volume
75
Issue
2
