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    Amantadinin alt ekstremite iskemi/reperfüzyon sıçan modelinde akciğer dokusu üzerindeki etkileri
    (2021) Orhan, Mustafa; Tuna, Ayça Taş; Ünal, Yusuf; Arslan, Mustafa; Yazar, Hayrullah; Sezen, Şaban Cem; Gözükara, Sezen Irmak
    Amaç: Bu çalışmada, sıçanlarda alt ekstremite iskemi/reperfüzyon\rhasarından sonra amantadinin akciğer dokusu üzerindeki etkisi araştırıldı.\rÇalışma\rplanı:\rToplam 24 adet Wistar cinsi sıçan her grupta altı\rsıçan olacak şekilde dört eşit gruba ayrıldı: Sham grubu (Grup S),\ramantadin grubu (Grup A), iskemi/reperfüzyon grubu (Grup I/R) ve\rI/R+amantadin grubu (Grup I/R-A). Tüm gruplara orta abdominal insizyon\ruygulandı. Grup I/R ve I/R-A?da infrarenal abdominal aorta 120 dk.\rsüreyle klemplendi ve ardından klemp kaldırılarak 120 dk. süreyle\rreperfüze edildi. Grup A ve I/R-A?daki sıçanlara 45 mg/kg amantadin\rhidroklorür cerrahiden 15 dk. önce intraperitoneal olarak uygulandı.\rReperfüzyon periyodu sonunda (240 dk.) tüm sıçanlar sakrifiye edildi ve\rakciğer dokuları alındı. Akciğer dokusunda katalaz ve süperoksit dismutaz\raktiviteleri ve glutatyon S-transferaz ve malondialdehit düzeyleri çalışıldı.\rAkciğer dokuları histopatolojik olarak incelendi.\rBulgular:\rKatalaz aktivitesi Grup A, I/R ve I/R-A?da, Grup S’ye kıyasla\rdaha düşüktü. Süperoksit dismutaz aktivitesi Grup I/R?de, Grup S?ye kıyasla\rdaha yüksekti. Grup I/R-A ve A?da, Grup S ve I/R?ye kıyasla, süperoksit\rdismutaz aktivitesi azaldı. Glutatyon S-transferaz düzeyleri Grup I/R ve\rA?da, Grup S’ye kıyasla azaldı. Glutatyon S-transferaz düzeyleri, Grup I/RA’da,\rGrup I/R ve A’ya kıyasla daha yüksekti. Malondialdehit düzeyleri,\rGrup I/R’de en yüksek ve Grup IR-A?da en düşük izlendi. Histopatolojik\rincelemeye göre, Grup S?de infiltrasyon skoru Grup I/R ve Grup I/R-A?ya\rkıyasla, anlamlı düzeyde daha düşüktü (sırasıyla. p=0.009 ve p=0.011).\rGrup I/R?de alveol duvar kalınlaşması skoru, Grup S ve Grup A?ya kıyasla,\ranlamlı düzeyde yüksekti (sırasıyla, p=0.001 ve p=0.001).\rSonuç:\rAkciğer dokusu iskemi/reperfüzyon hasarından etkilenebilir ve bu\rhasar amantadin kullanımı ile geri döndürülebilir.
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    Comparison of the efficacy of propofol and metoclopramide in preventing postoperative nausea and vomiting after middle ear surgery
    (Saudi Med J, 2009) Ünal, Yusuf; Özsoylar, Özgür; Arslan, Mustafa; Sarıgüney, Damla; Akçabay, Mehmet
    Objectives: To compare the administration of sub hypnotic dose of propofol with metoclopramide and placebo in prevention of postoperative nausea and vomiting (PONV) after middle ear surgery. Methods: This clinical research was performed in the Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey, between December 2004 and October 2005. Following approval by the hospital ethics committee, 60 adult patients scheduled for a middle ear operation were randomly assigned into 3 groups. The patients in group P received 0.5 mg.kg(-1) propofol; in group M, 0.2 mg.kg(-1) metoclopramide, and in group C, 0.9% saline solution. The number of patients suffering from nausea and vomiting at 0-4, 4-12, and 12-24 hours postoperatively, and additional use of antiemetics was recorded. Results: Comparison of the data showed that at 0-4th hours, the incidence of vomiting was 25% in group P, 40% in group M, and 75% in group C. The incidence rate of group P was significantly lower than that of group C (p=0.002), and the rate of antiemetics use in group C was higher than that in group P (p=0.028). The Nausea Vomiting Scale scores of group C were also significantly higher than those of group P (P=0.005). There were no significant differences between the values at 4-12 and 12-24 hours. Conclusion: The administration of a sub hypnotic dose of propofol at the end of surgery was found to be at least as effective as metoclopramide in preventing PONV in the early postoperative period in adult patients undergoing middle ear surgery.
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    Dexmedetomidine protects against lipid peroxidation and erythrocyte deformability alterations in experimental hepatic ischemia reperfusion injury
    (Taylor & Francis Ltd, 2012) Arslan, Mustafa; Comu, Faruk Metin; Kucuk, Aysegul; Ozturk, Levent; Yaylak, Faik
    Background: Hepatic ischemia-reperfusion injury is a common clinical problem in hepatic surgery and transplantation. Several cellular and tissue structural and functional alterations are observed in such injury. The aim of this study was to evaluate the effect of dexmedetomidine on lipid peroxidation and erythrocyte deformability during ischemia-reperfusion injury in rats. Methods: Twenty-four Wistar Albino rats were randomly separated into three groups as control (C), ischemia-reperfusion injury (I/R) and dexmedetomidine group (I/R-D). Ischemia was induced with portal clampage for 45 min and reperfusion period was 45 min after declampage. Group I/R-D received dexmedetomidine 100 mu g/kg i.p. 30 min before portal clampage. Serum malondialdehyde and superoxide dismutase activities to document lipid peroxidation and erythrocyte deformability index were investigated. Results: Serum superoxide dismutase and malondialdehyde activity levels were significantly higher and erythrocyte deformability index was decreased in hepatic ischemia-reperfusion group. However, these changes were observed to be prevented with dexmedetomidine treatment when given before portal clampage. Conclusion: These findings clearly indicate that erythrocyte deformability index is decreased in hepatic ischemia reperfusion injury and has a potential role to prevent these alterations. The protective effect of dexmedetomidine on hepatic I/R injury is also decreased lipid peroxidation. Further experimental and clinical investigations may clarify the molecular mechanisms and clinical significance of these findings.
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    Diyabetik Ratlarda Propofol ve C Vitamini Uygulamasının Karaciğer ve Böbrek Dokusu Üzerindeki Etkisinin Araştırılması
    (2015) Arslan, Mustafa; Bilge, Mustafa; Sezen, Şaban Cem; Öztürk, Levent; Işık, Berrin; Çomu, Faruk Metin; Alkan, Metin
    Amaç: Diyabet komplikasyonları ile lipid peroksidasyonu arasında yakın ilişki olduğu bilinmektedir. Diyabetik ratlarda propofol farmako-dinamisi ve farmako-kinetiğinin değiştiği gösterilmiştir. Bu çalışmada diyabetik ratlarda propofol ve C vitaminin karaciğer ve böbrek dokusuna etkisini araştırmayı amaçladık. Yöntemler: Çalışmada 28 Wistar Albino sıçan kullanıldı. Hayvanlar randomize olarak 4 gruba ayrıldı. Kontrol (K) grubuna sadece intraperitoneal salin verildi. Diyabet oluşturulacak 3 gruptaki hayvanlara ise tek doz streptozotosin verilerek (60 mg/kg) deneysel diyabet oluşturuldu. Diyabet-propofol grubu (DP) için hayvanlara intarperitoneal olarak 150 mg/kg propofol verildi. Diyabet-propofol ve C vitamini (DP+Vit C) verilen gruptaki hayvanlara 150 mg/kg propofol verilmeden 30 dakika önce 100 mg/kg C vitamini verildi. Diyabet Kontrol (DK) grubuna ise diyabet oluşturulduktan sonra sadece intraperitoneal salin verildi. İlaç uygulamadan sonra hayvanlar sakrifiye edilerek karaciğer ve böbrek doku preperatları histolojik ve biyokimyasal değerlendirme için hazırlandı. Antioksidan enzimler süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GST) aktiviteleri ve malondialdehid (MDA) konsantrasyonları karaciğer ve böbrek dokusunda ölçüldü. Bulgular: Karaciğer MDA düzeyleri; Diyabet kontrol (DK) grubunda DP, DP+Vit C ve K gruplarına göre yüksek bulundu (p=0.024, p=0.008, p=0.016). Kontrol grubu ve DP+ Vit C grubunda karaciğer SOD aktivitesi DK grubundan anlamlı olarak daha düşük bulundu (p=0.011, p=0.038). Kontrol grubu ile karşılaştırıldığında DP+ Vit C grubundaki karaciğer GST aktivitesi daha düşük olarak bulundu (p = 0.011). Karaciğer CAT aktivitesi açısından gruplar arasında anlamlı fark bulunamadı. DK grubundaki böbrek MDA düzeyleri DP+ Vit C ve K grubuna göre daha yüksek olarak bulundu (p=0.016, p=0.010). DK grubundaki böbrek SOD aktivitesi diğer üç gruba göre daha düşük bulundu (p=0.028, p=0.019, p=0.009). Böbrek dokusunda bakılan GST ve CAT aktiviteleri açısından gruplar arasında anlamlı fark bulunamadı. DP grubundaki histopatolojik hasarlanma düzeyi kontrol grubundan anlamlı olarak yüksek bulundu. Sonuç: Diyabet kliniğinde lipid peroksidasyonu artmakta ve antioksidan aktivite azalmaktadır. Bununla birlikte C vitamini uygulaması bu durumdaki lipid peroksidasyonunu azaltırken antioksidan aktiviteyi de artırmaktadır. Çalışmamızın sonuçlarının diğer deneysel çalışmalarla desteklenmesi gerekmektedir
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    Efect of Levosimendan and Nigella Sativa on Erythrocyte Deformability During Myocardial Ischaemia-Reperfusion Injury in Rats
    (Wiley, 2017) Ozer, Abdullah; Comu, Faruk Metin; Kucuk, Aysegul; Kilic, Yigit; Mardin, Baris; Alkan, Metin; Arslan, Mustafa
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    Effect of dexmedetomidine on ischemia-reperfusion injury of liver and kidney tissues in experimental diabetes and hepatic ischemia-reperfusion injury induced rats
    (Anaesthesia Pain & Intensive Care, 2016) Sezen, Saban Cem; Isik, Berrin; Bilge, Mustafa; Arslan, Mustafa; Comu, Faruk Metin; Ozturk, Levent; Kavutcu, Mustafa
    Background: Reperfusion following ischemia can lead to more injuries than ischemia itself especially in diabetic patients. The aim of this study was to evaluate the effect of dexmedetomidine on ischemia-reperfusion injury (IRI) in rats with have hepatic IRI and diabetes mellitus. Methodology: Twenty-eight Wistar Albino rats were randomised into four groups as control (C), diabetic (DC), diabetic with hepatic ischemia-reperfusion injury (DIR), and diabetic but administered dexmedetomidine followed by hepatic IRI (DIRD) groups. Hepatic tissue samples were evaluated histopathologically by semiquantitative methods. Malondialdehyde (MDA), superoxide dismutase (SOD), glutathion s-transpherase (GST), and catalase (CAT) enzyme levels were investigated in liver and kidney tissues as oxidative state parameters. Results: In Group DIR; hepatocyte degeneration, sinusoidal dilatation, pycnotic nucleus, and necrotic cells were found to be more in rat hepatic tissue; while mononuclear cell infiltration was higher in the parenchyme. MDA levels were significantly lower; but SOD levels were significantly higher in Group DIRD with regard to Group DIR. In the IRI induced diabetic rats' hepatic and nephrotic tissues MDA levels, showing oxidative injury, were found to be lower. SOD levels, showing early antioxidant activity, were higher. Conclusion: The enzymatic findings of our study together with the hepatic histopathology indicate that dexmedetomidine has a potential role to decrease IRI.
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    Effect of Dexmedetomidine on Lung Tissue Lower Extremity Ischemia Reperfusion Injury in Streptozotocin Induced Diabetic Rats
    (GAZI UNIV, FAC MED, 2020) Sezen, Saban Cem; Celik, Ilknur Aytekin; Aydin, Muhammed Enes; Ozterlemez, Naciye Turk; Arslan, Mustafa; Erbatur, Meral Erdal; Kavutcu, Mustafa
    Objective: The aim of our study was to investigate the effects of dexmedetomidine on lung tissue in rat's lower extremity after undergoing an ischemia reperfusion (I/R) injury. Material and methods: After obtaining ethical committee approval, 24 Wistar albino rats (200-270 gr) were randomly divided into four groups: (Control (Group C), diabetes-control (Group DC), diabetes I/R (Group DIR), and diabetes-I/R-dexmedetomidine (Group DIRD). In diabetes groups, single-dose (55 mg/kg) streptozotocin was administered intraperitoneally. Rats with a blood glucose level above 250 mg/dl at the 72nd hour were accepted as diabetic. At the end of four weeks, laparotomy was performed in all rats. Nothing else was done in Group C and DC. In Group DIR, ischemia reperfusion was produced via two-hour periods of clamping and subsequent declamping of infra-renal abdominal aorta. In Group DIRD, 100 mu g/kg of dexmedetomidine were administered intraperitoneally. Results: When the groups' lung tissue neutrophil infiltration/aggregation light microscopic findings were compared to each other, a significant difference was observed among the groups (p=0.003). When the groups' lung tissue injury score light microscopic findings were compared, a significant difference was observed among the groups (p=0.001). When groups were compared to each other in terms of lung tissue MDA levels and SOD activities, a significant difference was observed (p=0.002, p=0.018, respectively). Conclusion Our results confirm that dexmedetomidine has protective effects against the lung damage resulting from IR in diabetic rats. However, future studies should be conducted to evaluate these effects.
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    The effect of dexmedetomidine on myocardial ischemia reperfusion injury in streptozotocin induced diabetic rats
    (Anaesthesia Pain & Intensive Care, 2015) Arslan, Mustafa; Poyraz, Fatih; Kiraz, Hasan Ali; Alkan, Metin; Kip, Gülay; Erdem, Özlem; Çomu, Faruk Metin
    Objective: Ischemia/reperfusion (I/R) injury is an important cause of myocardial damage by means of oxidative, inflammatory, and apoptotic mechanisms. The aim of the present study was to examine the potential cardio-protective effects of dexmedetomidine in a diabetic rat model of myocardial I/R injury. Methodology: A total of 18 streptozotocin (55 mg/kg) induced diabetic Wistar Albino rats were randomly divided into three equal groups as follows: the diabetic I/R group (DIR) in which myocardial I/R was induced by ligating the left anterior descending (LAD) coronary artery for 30 min, followed by 2 hours of reperfusion following left thoracotomy, the diabetic I/R dexmedetomidine group (DIRD) which were given 100 mu g/kg dexmedetomidine intraperitoneally 30 min before I/R induction by the same method and the diabetic control group (DC) which underwent sham operations without tightening of the coronary sutures. As a control group (C), 6 healthy age-matched Wistar Albino rats underwent sham operations similar to DC group. After the operation the rats were sacrificied and the myocardial tissues were histopathologically examined. Results: Microscopic myonecrosis findings were significantly different among groups (p= 0.008). Myonecrosis findings were significantly higher in DIR compared to C, DC and DIRD groups (p= 0.001, p=0.007 and p=0.037 respectively). Similarly microscopic inflammatory cell infiltration degrees showed significant differences among groups (p<0.0001). Compared to C, DC and DIRD groups, the microscopic inflammatory cell infiltration was significantly higher among DIR group (p<0.0001, p<0.0001 and p=0.009 respectively). Also myocardial tissue edema was significantly different among groups (p=0.002). The microscopic myocardial tissue edema levels were significantly higher in DIR group than C and DIRD groups (p<0.0001 and p=0.022 respectively). Tissue edema was also more prominent in DC compared to C group (p=0.022) Conclusion: Taken together our data indicate that dexmedetomidine may be helpful in reducing myocardial necrosis, myocardial inflammation and myocardial tissue edema resulting from ischemia/reperfusion injury.
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    The Effect of Different Doses Apelin 13 on Erythrocyte Deformability in Rats
    (Wiley, 2019) Dursun, Ali Dogan; Ozdemir, Cagri; Comu, Faruk Metin; Kucuk, Aysegul; Arslan, Mustafa
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    Effect of Exenatide on Liver in an Iron Overload Rat Model
    (Wiley-Blackwell, 2015) Kuskonmaz, Serife Mehlika; Dursun, Ali Dogan; Kara, Halil; Sarikaya, Badegul; Bayraktar, Aslihan Cavunt; Kucuk, Aysegul; Arslan, Mustafa
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    Effect of Fullerenol C60 on Erythrocyte Deformability During Ischaemia-Reperfusion Injury of Lower Extremity in Diabetic Rats
    (Gazi Univ, Fac Med, 2019) Kip, Gülay; Kartal, Hakan; Çomu, Faruk Metin; Polat, Yücel; Arslan, Mustafa; Küçük, Ayşegül
    Background: Fullerenol, a water-soluble C60-fullerene derivative synthesized by Chiang et al, has been demonstrated to be able to scavenge free radicals in vitro and in vivo. Although its protective effects have been already studied and shown in ischemia reperfusion (IR) injury, additional investigation is necessary for its effect on erythrocyte deformability. The purpose of our study was to look into the effects of fullerenol C60 on erythrocyte deformability in rat lower extremity ischemia reperfusion injury model. Materials and Methods: After approval of the Ethics Committee, 30 Wistar Albino rat were divided into 5 groups (n:6) as; Control (C), Diabetes (group D), diabetes+ fullerenol C60 group (DF), diabetes+ IR (group DIR) and diabetes IR+ fullerenol C60 (DIRF). 55 mg/kg streptozotocin was administered to the rats for diabetes. After the period of 72 hour, blood glucose concentration was mesured, 250 mg/dl and above were considered as diabetic rat. Four week after the formation of diabetes, rats were subjected to 2 hour ischemia and 2 hour reperfusion. Erythrocyte packs were prepared from heparinized blood samples and deformability measurements were performed. Results: The deformability index was significantly increased in diabetic rats; however, it was similar in group D, DF and DIRF. It was significantly increased in group DIR when compared to group C, D, DF and DIRF. The relative resistance was increased in I/R models. Conclusion: This study aimed to investigate the effects of IR on erythrocyte deformability which may lead to disturbance in blood flow and hence tissue perfusion in infrarenal rat aorta. We found that fullerenol C60 had beneficial effects by reversing undesirable effects of IR. In our opinion, further studies with larger volume are required to support our promising results.
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    Effect of Irisin on Erythrocyte Deformability in Mice with Lower Limb Ischemia Reperfusion Injury
    (Wiley, 2017) Polat, Yucel; Comu, Faruk Metin; Kucuk, Aysegul; Kartal, Hakan; Dursun, Ali Dogan; Arslan, Mustafa
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    The effect of levosinnendan on myocardial ischemia reperfusion injury in streptozotocin-induced diabetic rats
    (Co-Action Publishing, 2015) Kiraz, Hasan Ali; Poyraz, Fatih; Kip, Gulay; Erdem, Ozlem; Alkan, Metin; Arslan, Mustafa; Comu, Faruk Metin
    Objective: Ischemia/reperfusion (I/R) injury is an important cause of myocardial damage by means of oxidative, inflammatory, and apoptotic mechanisms. The aim of the present study was to examine the potential cardio protective effects of levosimendan in a diabetic rat model of myocardial I/R injury. Methods: A total of 18 streptozotocin-induced diabetic Wistar Albino rats (55 mg/kg) were randomly divided into three equal groups as follows: the diabetic I/R group (DIR) in which myocardial I/R was induced following left thoracotomy, by ligating the left anterior descending coronary artery for 60 min, followed by 2 h of reperfusion; the diabetic I/R levosimendan group (DIRL), which underwent I/R by the same method while taking levosimendan intraperitoneal 12 mu g kg(-1); and the diabetic control group (DC) which underwent sham operations without tightening of the coronary sutures. As a control group (C), six healthy age-matched Wistar Albino rats underwent sham operations similar to the DC group. Two hours after the operation, the rats were sacrificed and the myocardial tissue samples were examined by light microscopy for evidence of myonecrosis and inflammatory cell infiltration. Results: Myonecrosis findings were significantly different among groups (p = 0.008). Myonecrosis was more pronounced in the DIR group compared with the C, DC, and DIRL groups (p = 0.001, p = 0.007 and p = 0.037, respectively). Similarly, the degree of inflammatory cell infiltration showed significant difference among groups (p < 0.0001). Compared with C, DC, and DIRL groups, the inflammatory cell infiltration was significantly higher among the DIR group (p < 0.0001, p < 0.0001, and p = 0.020, respectively). Also, myocardial tissue edema was significantly different among groups (p = 0.006). The light microscopic myocardial tissue edema levels were significantly higher in the DIR group than the C, DC, and DIRL groups (p = 0.001, p = 0.037, and p = 0.014, respectively). Conclusion: Taken together, our data indicate that levosimendan may be helpful in reducing myocardial necrosis, myocardial inflammation, and myocardial tissue edema resulting from ischemia reperfusion injury.
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    Effect of picroside II on erythrocyte deformability and lipid peroxidation in rats subjected to hind limb ischemia reperfusion injury
    (Dove Medical Press Ltd, 2016) Comu, Faruk Metin; Kilic, Yigit; Ozer, Abdullah; Kirisci, Mehmet; Dursun, Ali Dogan; Tatar, Tolga; Arslan, Mustafa
    Background: Ischemia reperfusion injury (I/R) in hind limb is a frequent and important clinical phenomenon. Many structural and functional damages are observed in cells and tissues in these kinds of injuries. In this study, we aimed to evaluate the effect of picroside II on lipid peroxidation and erythrocyte deformability during I/R in rats. Methods: Rats were randomly divided into four groups-each containing six animals (sham, I/R, sham + picroside II, and I/R + picroside II). The infrarenal section of the abdominal aorta was occluded with an atraumatic microvascular clamp in I/R groups. The clamp was removed after 120 minutes and reperfusion was provided for a further 120 minutes. Picroside II (10 mg.kg(-1)) was administered intraperitoneally to the animals in the appropriate groups (sham + picroside II, I/R + picroside II groups). All rats were euthanized by intraperitoneal administration of ketamine (100 mg.kg(-1)) and taking blood from the abdominal aorta. Erythrocytes were extracted from heparinized complete blood samples. Buffer (PT) and then erythrocytes (PE) were passed through the filtration system and the changes in pressure were measured to investigate the role of serum malondialdehyde and nitric oxide (NO) in lipid peroxidation and erythrocyte deformability index. Results: Deformability index was significantly increased in the I/R group compared to groups sham, sham + picroside-II, and I/R + picroside-II (P<0.0001, P<0.0001, and P=0.007). Malondialdehyde (MDA) and NO levels were evaluated. MDA level and NO activity were also higher in the I/R group than in the other groups. Picroside II treatment before hind limb I/R prevented these changes. Conclusion: These results support that deformability of erythrocytes is decreased in I/R injury and picroside II plays a critical role to prevent these alterations. Further experimental and clinical studies are needed to evaluate and clarify the molecular mechanisms of action and clinical importance of these findings.
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    Effect of Picroside-2 on Erythrocyte Deformability and Lipid Peroxidation in Rats Subjected to Lower Extremity Ischemia-reperfusion Injury
    (Wiley-Blackwell, 2015) Comu, Faruk Metin; Kilic, Yigit; Ozer, Abdullah; Kirisci, Mehmet; Dursun, Ali Dogan; Tatar, Tolga; Arslan, Mustafa
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    The Effect of Picroside-2 on Erythrocyte Deformability and Lipid Peroxidation in Streptozotocin-Induced Diabetic Rats subjected to Left Anterior Descending Artery-Ischemia reperfusion (conferenceObject)
    (Wiley-Blackwell, 2015) Comu, Faruk Metin; Polat, Yucel; Ozer, Abdullah; Erer, Dilek; Kirisci, Mehmet; Dursun, Ali Dogan; Arslan, Mustafa
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    Effects of Amantadine on Liver and Lung Tissue in Hepatic Ischemia Reperfusion Injury in Rats
    (Gazi Univ, Fac Med, 2023) Sahin, Fatih; Tuna, Ayca Tas; Unal, Yusuf; Arslan, Mustafa; Yazar, Hayrullah; Sezen, Saban Cem; Gozukara, Sezen Irmak
    Background: N-Methyl D-Aspartate (NMDA) receptor blockers have been shown to have protective effects against ischemia/reperfusion (I/R) injury in various tissues. The aim of this study was to investigate the effects of amantadine on liver and lung tissue in hepatic I/R injury. Materials and Methods: Twenty-four rats divided into 4 groups: the Sham Group (S), the Amantadine Group (A), the I/R Group (I/R) and the I/R + Amantadine Group (I/R-A). In Group A and Group I/R-A, 45 mg/kg of amantadine was administered before surgery. In Group I/R and Group I/R-A, an atraumatic vascular clamp was applied to the structures in the left portal triad for 45 minutes and reperfusion period was 2 hours after declampage. Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) enzyme levels were were studied in liver and lung tissues. Additionally tissues were examined histopathologically. Results: No significant difference was observed in tissue MDA, SOD, and CAT levels among four groups (p >0.05). Polymorphonuclear cell infiltration and the scores of hepatocyte degeneration, sinusoidal dilatation, pycnotic core, and necrosis cell were significantly higher in Group I/R than other groups (p<0.05). Regarding to the lung tissue, the neutrophil/lymphocyte infiltration score was significantly lower in Group S and A than in Group I/R (respectively; p= 0.007, 0.011), and it was significantly higher in Group I/R-A than in Group S (p = 0.014). The alveolar wall thickening score was significantly higher in Group I/R than the other groups (p <0.0001). Conclusion: Amantadine may have a protective effect against I/R damage, as it reduces histopathological changes caused by I/R damage.
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    Effects of Carvacrol on Erythrocyte Deformability in Lower Extremity Ischemia Reperfusion Induced Rats
    (Wiley, 2018) Comu, Faruk Metin; Ozer, Abdullah; Mardin, Baris; Arslan, Mustafa; Kucuk, Aysegul
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    Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia
    (Bmc, 2023) Gobut, Huseyin; Kucuk, Aysegul; Sengel, Necmiye; Arslan, Mustafa; Ozdemir, Cagri; Mortas, Tulay; Kasapbasi, Esat
    IntroductionDuring liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion injury (IRI). Nanomedicine uses the latest technology, which has emerged with interdisciplinary effects, such as biomedical sciences, physics, and engineering, to protect and improve human health. Interdisciplinary research has brought along the introduction of antioxidant nanoparticles as potential therapeutics. The goal of this study was to investigate the effects of cerium oxide (CeO2) administration and desflurane anesthesia on liver tissue in liver IR injury.Material and methodsThirty rats were randomly divided into five groups: control (C), ischemia-reperfusion (IR), IR-desflurane (IRD), cerium oxide-ischemia reperfusion (CeO2-IR), and cerium oxide-ischemia reperfusion-desflurane (CeO2-IRD). In the IR, IRD, and CeO2-IRD groups, hepatic ischemia was induced after the porta hepatis was clamped for 120 min, followed by 120 min of reperfusion. Intraperitoneal 0.5 mg/kg CeO2 was administered to the CeO2 groups 30 min before ischemia. Desflurane (6%) was administered to the IRD and CeO2-IRD groups during IR. All groups were sacrificed under anesthesia. Liver tissue samples were examined under a light microscope by staining with hematoxylin-eosin (H&E). Malondialdehyde (MDA) levels, catalase (CAT), glutathione-s-transferase (GST), and arylesterase (ARE) enzyme activities were measured in the tissue samples.ResultsThe IR group had considerably more hydropic degeneration, sinusoidal dilatation, and parenchymal mononuclear cell infiltration than the IRD, CeO2-IR, and CeO2-IRD groups. Catalase and GST enzyme activity were significantly higher in the CeO2-IR group than in the IR group. The MDA levels were found to be significantly lower in the IRD, CeO2-IR, and CeO2-IRD groups than in the IR group.ConclusionIntraperitoneal CeO2 with desflurane reduced oxidative stress and corrected liver damage.
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    Effects of cerium oxide on liver tissue in liver ischemia-reperfusion injury in rats undergoing sevoflurane anesthesia
    (Spandidos Publ Ltd, 2023) Gobut, Huseyin; Erel, Selin; Ozdemir, Cagri; Mortas, Tulay; Arslan, Mustafa; Kucuk, Aysegul; Kasapbasi, Esat
    During liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion (IR) injury. Nanomedicine uses the latest technological advancement, which has emerged from interdisciplinary efforts involving biomedical sciences, physics and engineering to protect and improve human health. Antioxidant nanoparticles are potential therapeutic agents. The present study investigated the effects of cerium oxide (Co) administration and sevoflurane anesthesia on liver tissue with IR injury. A total of 36 rats were randomly divided into control, Co, IR, IR-Sevoflurane (IRS), Co + IR and Co + IRS groups. In the IR, IRS and Co + IRS groups, hepatic IR was induced. Intraperitoneal Co was administered to the Co groups 30 min before ischemia. Sevoflurane was administered to the IRS and Co + IRS groups during IR injury. Liver tissue samples were examined under the light microscope by staining with hematoxylin and eosin. Thiobarbituric acid (TBARS) levels as well as catalase (CAT) and glutathione-S-transferase (GST) enzyme activity were evaluated in liver tissue samples. The IR group had considerably more hydropic degeneration, sinusoidal dilatation and parenchymal neutrophil infiltration than the Co, IRS, Co + IR and Co + IRS groups. CAT and GST enzyme activity were significantly higher in Co and Co + IR groups compared with the IR group. TBARS levels were significantly lower in Co, IRS, Co + IR and Co + IRS groups compared whit those in the IR group. Intraperitoneal injection of Co with sevoflurane decreased oxidative stress and damage to the liver.