Effect of Dexmedetomidine on Lung Tissue Lower Extremity Ischemia Reperfusion Injury in Streptozotocin Induced Diabetic Rats
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
GAZI UNIV, FAC MED
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: The aim of our study was to investigate the effects of dexmedetomidine on lung tissue in rat's lower extremity after undergoing an ischemia reperfusion (I/R) injury. Material and methods: After obtaining ethical committee approval, 24 Wistar albino rats (200-270 gr) were randomly divided into four groups: (Control (Group C), diabetes-control (Group DC), diabetes I/R (Group DIR), and diabetes-I/R-dexmedetomidine (Group DIRD). In diabetes groups, single-dose (55 mg/kg) streptozotocin was administered intraperitoneally. Rats with a blood glucose level above 250 mg/dl at the 72nd hour were accepted as diabetic. At the end of four weeks, laparotomy was performed in all rats. Nothing else was done in Group C and DC. In Group DIR, ischemia reperfusion was produced via two-hour periods of clamping and subsequent declamping of infra-renal abdominal aorta. In Group DIRD, 100 mu g/kg of dexmedetomidine were administered intraperitoneally. Results: When the groups' lung tissue neutrophil infiltration/aggregation light microscopic findings were compared to each other, a significant difference was observed among the groups (p=0.003). When the groups' lung tissue injury score light microscopic findings were compared, a significant difference was observed among the groups (p=0.001). When groups were compared to each other in terms of lung tissue MDA levels and SOD activities, a significant difference was observed (p=0.002, p=0.018, respectively). Conclusion Our results confirm that dexmedetomidine has protective effects against the lung damage resulting from IR in diabetic rats. However, future studies should be conducted to evaluate these effects.
Açıklama
Celik, ilknur Aytekin/0000-0003-0754-680X;
Anahtar Kelimeler
Dexmedetomidine, ischemia reperfusion, lung, rat
Kaynak
GAZI MEDICAL JOURNAL
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
31
Sayı
3
Künye
Sezen, Ş. C., Aytekin Çelik, İ., Aydın, M. E., Türk Özterlemez, N., Arslan, M., Erdal Erbatur, M., & Kavutçu, M. (2020). Effect of Dexmedetomidine on Lung Tissue Lower Extremity Ischemia Reperfusion Injury in Streptozotocin Induced Diabetic Rats. Gazi Medical Journal, 31(3), 358–362.
