Yazar "Atmaca, Hasan Tarik" seçeneğine göre listele
Listeleniyor 1 - 20 / 20
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Central nervous system and skull malformations associated with Bovine Viral Diarrhea Virus in a calf(Ankara Univ Press, 2012) Atmaca, Hasan Tarik; Dincel, Gungor Cagdas; Kumandas, Ali; Kul, Oguz; Orhan, Ismail OnderIn this study, cranial anomalies, hydranencephaly and cerebellar hypoplasia were reported macroscopically, microscopically and immunohistochemically in a calf. A relationship between Bovine Viral Diarrhea Virus infection and malformations associated with central nervous system lesions were demonstrated.Öğe Clinicopathologic diagnosis of cutaneous toxoplasmosis in an Angora cat(Schluetersche Verlagsgesellschaft Mbh & Co Kg, 2011) Kul, Oguz; Atmaca, Hasan Tarik; Deniz, Ahmet; Suer, CenkThis report deals with a case of cutaneous toxoplasmosis in a 2 year-old female Angora cat. Cutaneous lesions were characterized by prescapular ulcers and hyperemic nodules in the skin of the inguinal and dorsosacral regions. A skin biopsy sample was collected from the lesioned area and processed for histopathologic examination and immunoperoxidase test using Toxoplasma gondii and Neospora caninum specific antibodies. Toxoplasma gondii immunopositive reactions were detected in keratinocytes and dermal macrophages while no immunoreactivity was detected for N. caninum. The case of cutaneous toxoplasmosis was further confirmed by PCR analysis using T gondii B1 gene-specific primers. In conclusion, we report the first case of cutaneous toxoplasmosis in Angora cats.Öğe Does Bosentan Protect Diabetic Brain Alterations in Rats? The Role of Endothelin-1 in the Diabetic Brain(Wiley, 2015) Demir, Recep; Cadirci, Elif; Akpinar, Erol; Cayir, Yasemin; Atmaca, Hasan Tarik; Un, Harun; Demir, IlknurDiabetes mellitus (DM) is a major problem all over the world, affecting more people in recent years. Individuals with diabetes are more prone to disease than non-diabetics, especially vascular complications. The aim of this study was to examine the roles of the endothelin (ET)-1 in brain damage formed in a streptozocin (STZ)-induced diabetes model, and the effect of bosentan, which is the non-specific ET1 receptor blocker in the prevention of the diabetes-induced brain damage. To examine the effects of bosentan (50mg/kg and 100mg/kg) in this study, the rats were given the drug for 3months. The rats were divided into four groups: the sham group (n=10), the diabetic control group (n=10), the group of diabetic rats given bosentan 50mg/kg (n=10) and the group of diabetic rats given bosentan 100mg/kg (n=10). Diabetes was induced in the rats by STZ (60mg/kg i.p.). On day 91, all rats were killed. Brain tissues of the rats were measured by molecular, biochemical and histopathological methods. Antioxidant levels in the therapy groups were observed as quite near to the values in the healthy group. In this study, while the brain eNOS levels in the diabetic groups decreased, the ET1 and iNOS levels were found to be increased. However, in the diabetes group, hippocampus and cerebellum, pericellular oedema and a number of neuronal cytoretraction were increased in neuropiles, whereas these results were decreased in the therapy group. Based on all of these results, ET1 will not be ignored in diabetes-induced cerebral complications.Öğe The Effects of Urotensin II Agonist and Antagonist on the Testicular Damage of Streptozotocin-induced Diabetic Rats(Wiley-Blackwell, 2016) Sengul, Emin; Gelen, Volkan; Celebi, Fikret; Cinar, Ali; Atmaca, Hasan Tarik…Öğe Evaluation of oxidative stress, hematological and biochemical parameters during Toxoplasma gondii infection in gerbils(Ankara Univ Press, 2015) Atmaca, Nurgul; Cinar, Miyase; Guner, Bayram; Kabakci, Ruhi; Gazyagci, Aycan Nuriye; Atmaca, Hasan Tarik; Canpolat, SilaThe aim of the present study was to investigate the alterations of oxidative stress, hematological and biochemical parameters in experimental infection caused by Toxoplasma gondii in gerbil. A total of 16 gerbil, 8 of which were control and 8 was infection group, were used in the study. The gerbils were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. In group of, the gerbil were sacrificed at 7th day after inoculation. At the end of this period, blood samples collected and erythrocyte malondialdehyde (MDA) concentrations, superoxide dismutase (SOD), catalase (CAT) activities, plasma aspartat aminotransferase (AST) and alanine aminotranspherase (ALT) activities, total protein, albumin, globulin were determined. Besides, hematological parameters were analysed in whole blood. Aspartat aminotransferase and ALT activities and MDA concentrations and neutrophil percentage and total leukocyte counts increased significantly in infected group when compared to control. In infected group, SOD activities, albumin concentrations and lymhocyte percentage decreased when compared to control. The results of this study suggest that oxidative stress, hematological and biochemical alterations may contribute to the pathogenesis of toxoplasmosis in gerbils.Öğe Experimental Chronic Toxoplasmosis Model in Mice: Brain Lesions and Related Behavioral Changes(Galenos Yayincilik, 2012) Kocak, Orhan Murat; Atmaca, Hasan Tarik; Terzi, Osman Safa; Buyukkayaer, Seyhan; Ozdemir, Hatice; Uzunalioglu, Tuba; Kul, OguzObjective: In this study, it is aimed to constitute a chronic toxoplasmosis model using toxoplasma gondii (T. gondii) ME49 strain that is supposed to produce tissue cysts, to compare healthy and infected mice in terms of behavioral changes. In addition, the relationship between behavioral changes and brain lesions was questioned. Method: Before experimental application, out of a total of twenty-one 2-month-old Swiss albino mice, T. gondii antibody-free, 14 were infected by intraperitoneal (IP) (n=8) or oral (n=6) inoculation of 2x102 Toxoplasma gondii ME49 oocysts. Four months later, fear-and anxiety-related behavioral changes in infected and healthy control groups were comparatively evaluated with plus-maze test. At the end of the experiment (45 day after inoculation), euthanasia was carried out on all mice and their brains were examined histopathologically and immunohistochemically for the presence of T. gondii tissue cysts. Results: Infected mice had low levels of anxiety, they have entered the open arms more frequently and have spent more time in the open arms of the maze compared to controls. Pathologically, in the infected group, high rates of gliosis, perivascular cell infiltration, meningitis, and neuron necrosis were observed especially in the parietal and temporal lobes, cornu ammonis, amygdala, and thalamus compared to the other parts of the brain. A higher number of tissue cyst formations were positively correlated with the lesion severity in these parts of the brain. Conclusion: It can be suggested that, tissue cysts and neuropathological changes in chronically infected mice brains have direct concern with behavioral manipulation which results from low anxiety levels. (Archives of Neuropsychiatry 2012; 49: 139-144)Öğe Hepatic stellate cells increase in Toxoplasma gondii infection in mice(Bmc, 2013) Atmaca, Hasan Tarik; Gazyagci, Aycan Nuriye; Canpolat, Sila; Kul, OguzBackground: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. Methods: Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. The mice were sacrificed in groups of 5 at 2, 4, and 6 days after inoculation. Another group of 5 mice were used as the controls. Anti-glial fibrillary acidic protein (GFAP) and anti-T. gondii antibodies were used to compare GFAP-immunoreactive cells and anti-T. gondiiimmunopositive areas in the liver between the T. gondii-infected groups and the healthy controls, respectively. Results: There was a significant correlation between the numbers of GFAP-positive hepatic stellate cells (HSCs) when they were compared with T. gondii antigen immunostaining (p < 0.05). The amount of T. gondii immunostaining increased significantly with the increase in the number of HSCs. Conclusions: There is a significant relationship between the number of HSCs and T. gondii antigens, which may represent an active role of HSCs in liver pathology and the pathobiology of T. gondii-related hepatitis.Öğe Increased expressions of ADAMTS-13 and apoptosis contribute to neuropathology during Toxoplasma gondii encephalitis in mice(Wiley, 2016) Dincel, Gungor Cagdas; Atmaca, Hasan TarikToxoplasma gondii (T. gondii) is a protozoan parasite with the potential of causing severe encephalitis among immunocompromised humans and animals. Our previous study showed that T. gondii induces high nitric oxide (NO) production, high glial activation (GFAP) and neurofilament expressions, leading to severe neurodegeneration in toxoplasma encephalitis (TE) in the central nervous system (CNS). The aim of this experimental study was to investigate ADAMTS-13 expression and apoptosis in CNS and to identify whether they have any correlation with toxoplasmosis neuropathology and neurodegeneration. Mice were infected with ME49 strain T. gondii and the levels of ADAMTS-13, caspase 3, caspase 8, caspase 9, TNFR1 and Bcl-xL expressions were examined in brain tissues by immunohistochemistry, during the development and establishment of chronic infections at 10, 30 and 60days post-infection. Results of the study revealed that the levels of ADAMTS-13 (P < 0.005), caspase 3 (P < 0.05), caspase 8 (P < 0.05), caspase 9 (P < 0.005) and TNFR1 (P < 0.05) expressions in the brain markedly increased while Bcl-xL expression decreased (P < 0.005). The most prominent finding from our study was that 10, 30 and 60days post-infection ADAMTS-13 increased significantly and this may play an important role in the regulation and protection of the blood-brain barrier integrity and CNS microenvironment in TE. These results also suggest that T. gondii-mediated apoptosis might play a pivotal role and a different type of role in the mechanism of neurodegeneration and neuropathology in the process of TE. Furthermore, expression of ADAMTS-13 might give an idea of the progress and is critical for diagnosis of this disease. To the best of the authors' knowledge, this is the first report on ADAMTS-13 expression in the CNS of T. gondii-infected mice.Öğe A new approach to Neospora caninum infection epidemiology: neosporosis in integrated and rural dairy farms in Turkey(Scientific Technical Research Council Turkey-Tubitak, 2014) Ocal, Naci; Atmaca, Hasan Tarik; Albay, Metin Koray; Deniz, Ahmet; Kalender, Hakan; Yildiz, Kader; Kul, OguzBoth cattle and dogs were examined in modern and rural dairy farms that had a history of abortion over 5%. The blood samples were collected from 427 aborted cattle and the sera were tested using a commercial ELISA test kit. Additionally, a necropsy procedure was carried out on the fetuses and calves dead within 2 months after birth; the tissue samples were evaluated by histopathologic, immunoperoxidase, and PCR techniques. Eighteen dogs in close contact with the cattle in the same field were included in the study and blood and feces samples were collected. The feces samples were analyzed by copro-PCR and the sera were tested by indirect fluorescent antibody test. As a result, 161 out of 427 sera samples (37.7%) were found positive for N. caninum. In cattle, the lowest seropositivity was 6.7% and the highest seropositivity was 74.24%. Neosporosis seroprevalence in integrated holdings was lower than those of rural dairy cattle facilities (66.7%). The seropositivity for N. caninum in dogs was determined as 72.7% in rural holdings and 28.6% in integrated holdings. According to the risk analysis, N. caninum-seropositive cows had greater exposure to N. caninum-seropositive dogs in rural family holdings and integrated holdings (P = 0.054, odds ratio = 0.929; and P= 0.008, odds ratio = 0.986, respectively).Öğe Nitric oxide production increases during Toxoplasma gondii encephalitis in mice(Academic Press Inc Elsevier Science, 2015) Dincel, Gungor Cagdas; Atmaca, Hasan TarikToxoplasma gondii is an intracellular parasite with the potential of causing severe encephalitis among immunocompromised human and animals. The aim of this experimental study was to investigate the immunomodulatory and immunopathological role of nitric oxide (NO) in central nervous systems and to identify any correlation between toxoplasmosis neuropathology and investigate the consequences of the cellular responses protect against T. gondii. Mice were infected with ME49 strain T. gondii and levels of endothelial, neuronal and inducible nitric oxide synthase (eNOS, nNOS, iNOS), glial fibrillary acidic protein (GFAP) and neurofilament (NF) were examined in brain tissues by immunohistochemistry, during the development and establishment of a chronic infection at 10 30 and 60 days post infection. Results of the study revealed that the levels of eNOS (p < 0.05), nNOS (p < 0.05), iNOS (p < 0.005), GFAP (p < 0.005) and NF (p < 0.005) were remarkably higher in T. gondii-infected mice than in uninfected control. The most prominent finding from our study was 10 and 30 days after inoculation data indicating that increased levels of NO not only a potential neuroprotective role for immunoregulatory and immunopathological but also might be a molecular trigger of bradyzoite development. Furthermore, this findings were shown that high expressed NO origin was not only inducible nitric oxide synthase but also endothelial and neuronal. We demonstrated that activation of astrocytes and microglia/macrophages is a significant event in toxoplasma encephalitis (TE). The results also clearly indicated that increased levels of NO might contribute to neuropathology related with TE. Furthermore, expression of NF might gives an idea of the progress and critical for diagnostic significance of this disease. (C) 2015 Elsevier Inc. All rights reserved.Öğe The Ocular Endothelin System: A Novel Target for the Treatment of Endotoxin-Induced Uveitis With Bosentan(Assoc Research Vision Ophthalmology Inc, 2014) Keles, Sadullah; Halici, Zekai; Atmaca, Hasan Tarik; Yayla, Muhammed; Yildirim, Kenan; Ekinci, Metin; Bayraktutan, ZaferPURPOSE. We compared the anti-inflammatory effects of bosentan and dexamethasone in endotoxin-induced uveitis (EIU). METHODS. Endotoxin-induced uveitis was induced by subcutaneous injection of lipopolysaccharide (LPS, 200 mu g) in Wistar rats. Rats were divided randomly into 10 groups (n=6). Bosentan at doses of 50 and 100 mg/kg were administered orally 1 hour before and 12 hours after LPS injection, and dexamethasone was administered by intraperitoneally 30 minutes before and 30 minutes after LPS injection at a dose of 1 mg/kg. Data were collected at two time points for each control and treatment; animals were killed at either 3 or 24 hours after LPS injection. Histopathologic evaluation and aqueous humour measurements of TNF-alpha level were performed, and endothelin-1 (ET-1), inducible nitric oxide synthase (iNOS), and endothelin receptor A and B (EDNRA and B) expression were analyzed. RESULTS. The group treated with 100 mg/kg bosentan at 24 hours displayed significantly milder uveitis and fewer inflammatory cells compared to LPS-injected animals, and there were similar findings in the dexamethasone-treated group at 24 hours. The TNF-alpha levels in the dexamethasone treatment group were lower than those in the LPS-induced uveitis control group (P < 0.05); however, there was no difference between the dexamethasone and bosentan treatment groups at 3 and 24 hours after LPS administration. Bosentan treatment at doses of 50 and 100 mg/kg significantly decreased iNOS expression compared to LPS-injected animals (P < 0.001). The ET-1 expression was suppressed significantly by bosentan and dexamethasone at 3 and 24 hours after LPS administration (P < 0.001). The EDNRA expression in the bosentan treatment groups was statistically significantly lower than that in the LPS-induced uveitis control group at 3 and 24 hours after LPS administration (P < 0.05). CONCLUSIONS. Bosentan reduces intraocular inflammation and has similar effects as dexamethasone in a rat model of EIU.Öğe Paracetamol-induced nephrotoxicity and oxidative stress in rats: the protective role of Nigella sativa(Taylor & Francis Ltd, 2016) Canayakin, Dogukan; Bayir, Yasin; Baygutalp, Nurcan Kilic; Karaoglan, Esen Sezen; Atmaca, Hasan Tarik; Ozgeris, Fatma Betul Kocak; Halici, ZekaiContext Nigella sativa L. (Ranunculaceae) (NS) is traditionally used to treat many conditions such as inflammation. Objective This study evaluates the effects of NS seeds ethanol extract in paracetamol-induced acute nephrotoxicity in rats. Materials and methods Forty-eight female Wistar Albino rats were divided into eight groups: I = sham; II = sham + 1000 mg/kg NS; III = sham + 140 mg/kg (N-acetyl cysteine) NAC; IV = 2 g/kg paracetamol; V = 2 g/kg paracetamol + 140 mg/kg NAC; VI, VII and VIII = 2 g/kg paracetamol + 250, 500 and 1000 mg/kg NS, respectively. Paracetamol administration (oral) was carried out 1 h after NS and NAC administrations (oral), and all animals were sacrificed 24 h later. Results Paracetamol administration significantly increased serum urea (88.05 U/L) and creatinine (0.80 U/L) when compared with the sham group (49.80 and 0.31 U/L, respectively). However, serum urea level was reduced to 65.60, 56.00 and 54.18 U/L, with 250, 500 and 1000 mg/kg doses of the extract, respectively. Also, serum creatinine level was reduced to 0.64, 0.57 and 0.52 U/L with 250, 500 and 1000 mg/kg doses of the extract, respectively. NS administration increased superoxide dismutase and glutathione, and decreased malondialdehyde levels in the kidneys. Kidney histopathological examinations showed that NS administration antagonized paracetamol-induced kidney pathological damage. Discussion and conclusions The results suggest NS has a significant nephroprotective activity on paracetamol-induced nephrotoxicity. It may be suggested that the antiinflammatory and antioxidant effects of NS ethanolic extract originated from different compounds of its black seeds.Öğe Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity in rats(Pergamon-Elsevier Science Ltd, 2014) Atmaca, Nurgul; Atmaca, Hasan Tarik; Kanici, Ayse; Anteplioglu, TugceProtective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity were studied in rats. A total of 28 Wistar albino male rats were used. Four study groups were randomly formed with seven animals in each. The groups were treated for 21 days with distilled water (control group), with water containing 100 ppm fluoride (fluoride group), with resveratrol (12.5 mg/kg i.p., resveratrol group), or with 100 ppm fluoride + 12.5 mg/kg resveratrol i.p. (fluoride + resveratrol group). At the end of the trial, blood samples were collected by cardiac puncture and tissue samples were taken simultaneously. The total antioxidant and oxidant status in plasma and tissues as well as plasma 8-hydroxydeoxyguanosine levels were measured. Histopathological analyses of rat liver and brain tissues were performed in all groups to identify any changes. In the fluoride group, the total oxidant levels increased in plasma, liver and brain and total antioxidant levels decreased, as did the plasma 8-hydroxy-deoxyguanosine levels. These changes were prevented by co-administration of resveratrol. In addition, fluoride-associated severe histopathological changes in brain and liver tissues were not observed in the fluoride + resveratrol group. Consequently, these data suggested that resveratrol had beneficial effects in alleviating fluoride-induced oxidative stress. (C) 2014 Elsevier Ltd. All rights reserved.Öğe Reactivated and clinical Toxoplasma gondii infection in young lambs: Clinical, serological and pathological evidences(Elsevier Science Bv, 2012) Atmaca, Hasan Tarik; Ocal, Naci; Babur, Cahit; Kul, OguzIn this study, fatal toxoplasmosis with severe neurological clinical signs was evaluated in 20 lambs of a sheep flock containing 90 Akkaraman sheep, 60 lambs and 3 rams. The clinical signs in infected lambs (n = 20) included incoordination, head shaking, tremors, shaking up, difficulty walking and subsequent death. No incidence of abortion observed in pregnant ewes. Two lambs with severe clinical signs were euthanized and necropsied following clinical and hematological examinations. Blood samples were also collected from the mothers of dead lambs and rams for Sabin-Feldman dye test to detect Toxoplasma gondii-specific antibodies. T. gondii infection was further confirmed by PCR analysis using T. gondii B1 gene specific primers. The histopathological findings included non-purulent myositis with T. gondii-like tissue cysts in the heart and skeletal musculature, severe necrotic vasculitis and multifocal necroses in the brain, liver and lungs. T. gondii immunoreactivity was present in the lungs, liver and spleen as well as tissue cyst-like structures. In differential diagnosis, Neospora caninum infection was excluded by immunohistochemical and PCR analyses. According to current literature, there has been no previous report on clinical toxoplasmosis in newborn lambs or goat kids, and the number of reports on clinical toxoplasmosis in small ruminants is limited to two adult goats with fatality following systemic toxoplasmosis. Our study indicated that toxoplasmosis occurs in lambs with severe neurological signs and subsequent death. (C) 2012 Elsevier B.V. All rights reserved.Öğe The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii(Scientific Technical Research Council Turkey-Tubitak, 2014) Yildiz, Kader; Kul, Oguz; Gokpinar, Sami; Atmaca, Hasan Tarik; Gencay, Yilmaz Emre; Gazyagci, Aycan Nuriye; Gurcan, Ismayil SafaSkeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25-58 x 25-62 (mean 34 x 36) mu m. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.Öğe Role of oxidative stress in the pathophysiology of Toxoplasma gondii infection(Sage Publications Inc, 2016) Dincel, Gungor Cagdas; Atmaca, Hasan TarikOxidative stress (OS) plays an essential role in the pathogenesis of common neurodegenerative diseases. We have previously shown that Toxoplasma gondii (T. gondii) induces high nitric oxide (NO) production, glial activation, and apoptosis that altogether cause severe neuropathology in toxoplasma encephalitis (TE). The objective of this study was to investigate the cytotoxic effect of OS and to identify a correlation between the causes of T. gondii induced neuropathology. Expression levels of glutathione reductase (GR), Cu/Zn superoxide dismutase (SOD1), neuron specific enolase (NSE), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) were investigated. Results of the study revealed that the levels of GR (P <0.005) and NSE (P<0.001) expression in the brain tissue markedly increased while SOD1 activity decreased (P<0.001) in the infected group compared to the non-infected group. In addition, intense staining for 8-OHdG (P<0.05) was observed both in the nucleus and the cytoplasm of neurons and glial cells that underwent OS. These results were reasonable to suggest that T. gondii-mediated OS might play a pivotal role and a different type of role in the mechanism of neurodegeneration/neuropathology in the process of TE. The results also clearly indicated that increased levels of NO and apoptosis might contribute to OS-related pathogenesis of TE. As a result, OS and expression of NSE might give an idea of the disease progress and may have a critical diagnostic significance for patients with T. gondii infection.Öğe The correlation of TNF alpha levels with acute phase proteins in acute Toxoplasma gondii infection in mice(Academic Press Inc Elsevier Science, 2022) Atmaca, Nurgul; Atmaca, Hasan TarikAcute Toxoplasma gondii infections can influence the liver as well as other organs. Many cytokines and proteins play a role in the acute response against infection. Tumor necrosis factor alpha (TNF alpha) is a cytokine that plays a key function in stimulating hepatocytes to produce acute phase proteins. In this study, we investigated TNF alpha levels associated with the levels of macroglobulin, haptoglobin, hemopexin, C-reactive protein (CRP), albumin, serum amyloid alpha protein (SAA), and clusterin, which are acute phase proteins, in serum of mice with T. gondii infection. In the experiment, a total of 24 mice were used; 6 mice constituted the control group and 18 mice were infected with the RH strain. On the 2nd, 4th, and 6th days following the infection, 6 animals were euthanized, and their serums were collected. Compared to the control group, we observed a statistically significant decrease in albumin concentration in the group with T. gondii infection on the 6th day. Also, this group displayed a statistically significant, gradual increase in clusterin levels on the 2nd and 6th days, C-reactive protein levels on the 4th day, haptoglobin levels on the 2nd and 4th days, hemopexin levels on the 2nd day, serum amyloid A levels on the 2nd, 4th, and 6th days, and TNF-alpha levels on the 2nd, 4th, and 6th days (p < 0.05). TNF-alpha was strongly positively correlated with CRP, SAA, and clusterin, moderately positively correlated with hemopexin, and strongly negatively correlated with albumin. The increase in CRP, SAA, clusterin, and hemopexin levels on the 2nd day after infection, in parallel with the increase in TNF-alpha levels, indicates that these proteins can be considered as major acute phase proteins in acute T. gondii infection in mice. The data obtained here may be helpful for the diagnosis of T. gondii infection and for monitoring treatments.Öğe The prevalence of Sarcocystis species in sheep slaughtered in municipality slaughterhouse in Kırıkkale(2007) Ozkayhan, Meral Aydenizöz; Karaer, Zafer; Ilkme, Aycan Nuriye; Atmaca, Hasan TarikThis study was undertaken for the determination of Sarcocysts species in sheep slaughtered in Kirikkale Municipality Slaughterhouse. For this study, oesophageal, diaphragm and intercostal muscles were collected from randomly selected 112 sheep out of 1131 sheep (814 sheep and 317 lambs) that were slaughtered from October 2005 to May 2006. The samples were examined for macro and microcyst of Sarcocysts spp. Macrocysts and microcysts were found in 58.92% of the overall samples. Microcysts were found in 47.32% and macrocysts were perceived in 20.53% of the sheep that were under examination. The distribution of the microcysts with respective to the age of the sheep was studied and it has been observed that 16.12% (5 in every 31 lambs) of the lambs under 1 year old and 59.25% (48 in every 81 sheep) of the sheep equal or older than 1 year old had possessed microcysts. Sarcocystis ovicanis (47.32%) and S. arieticanis (1.23%) were the species with the highest and lowest number of recurrences respectively. Macrocysts were observed in every sheep over one age. In addition, randomly selected oesophagi with macrocyst were examined histopathologically. Sarcocystis sporocysts were not found in dog feces.Öğe Tnf-alpha inhibition by infliximab as a new target for the prevention of glycerol-contrast-induced nephropathy(Elsevier Science Bv, 2015) Saritemur, Murat; Un, Harun; Cadirci, Elif; Karakus, Emre; Akpinar, Erol; Halici, Zekai; Atmaca, Hasan TarikContrast medium-induced nephropathy (CIN) remains as a problem with high incidence and mortality rates. The aim of this study is to examine the roles of infliximab (INF) in the glycerol (GLY) and CIN model in rats. The rats were separated into five groups (n=8): Healthy, GLY, GLY+CM, GLY+CM+INF 5 mg/kg intraperitoneally (i.p.), and GLY+CM+INF 7 mg/kg (i.p.). Antioxidant levels in the therapy groups were observed to be quite similar to those in the healthy group. In this study, while the kidney TNF-alpha, TGF-1 beta and Caspase 3 gene expressions' levels increased in the nephrotoxic groups, these levels were found to have decreased in the treatment groups. Moreover, histopathologic examination showed that hyaline, haemorrhagic casts and necrosis were increased in nephrotoxicity group, whereas they decreased in the therapy group. Furthermore, TNF-alpha and NF-kappa B expression were decreased with infliximab administrated groups similar to control group. In conclusion, we suggest that infliximab have protective roles on CIN. (C) 2015 Elsevier B.V. All rights reserved.Öğe Tracking acute phase protein response during acute and chronic Toxoplasma gondii infection(Springernature, 2019) Atmaca, Hasan Tarik; Gazyagci, Aycan Nuriye; Terzi, Osman Safa; Dincel, Gungor Cagdas; Sumer, TugceToxoplasmosis is a disease caused by the protozoan Toxoplasma gondii, which occurs worldwide in mammals and birds. Brain is the primary target organ because Toxoplasma gondii is a ubiquitous intracellular parasite that causes most frequently life-threatening encephalitis in immunocompromised patients. Relation of tissue cysts number, histopathology score and acute phase proteins were investigated. In this study, 36 mice are infected with Me49 strain of Toxoplasma gondii. The control group has 6 healthy mice. After inoculation of Toxoplasma gondii, at 10., 15., 20., 30., 45., 60. days, 6 each mice euthanized after collection of blood samples. Hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin levels are determined by ELISA. Then, brain tissues were investigated histopathologically and lesions were scored. The average cyst numbers were determined by counting three samples (25 mu l each) of each brain homogenate under light microscopy. Inflammatory reaction was observed on day 10 days after inoculation (d.a.i.) The lesions were characterized by perivascular mononuclear cell infiltration, focal mononuclear cell infiltration in the meninges, and glial proliferation. Tissue cysts were observed in all Toxoplasma gondii-infected groups. The highest lesion score was observed at 60 d.a.i. And the most tissue cyst number were on day 30. d.a.i. Serum levels of hemopexin, haptoglobulin, macroglobulin, serum amyloid A and clusterin were significantly higher than the control group on day 10-20., 10., 10-30., 10.,10-45 d.a.i., respectively. High level of acute phase proteins in mice on certain days infected with Toxoplasma gondii was exhibited a relationship between brain lesions and tissue cysts.
