Experimental Chronic Toxoplasmosis Model in Mice: Brain Lesions and Related Behavioral Changes
Yükleniyor...
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Galenos Yayincilik
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: In this study, it is aimed to constitute a chronic toxoplasmosis model using toxoplasma gondii (T. gondii) ME49 strain that is supposed to produce tissue cysts, to compare healthy and infected mice in terms of behavioral changes. In addition, the relationship between behavioral changes and brain lesions was questioned. Method: Before experimental application, out of a total of twenty-one 2-month-old Swiss albino mice, T. gondii antibody-free, 14 were infected by intraperitoneal (IP) (n=8) or oral (n=6) inoculation of 2x102 Toxoplasma gondii ME49 oocysts. Four months later, fear-and anxiety-related behavioral changes in infected and healthy control groups were comparatively evaluated with plus-maze test. At the end of the experiment (45 day after inoculation), euthanasia was carried out on all mice and their brains were examined histopathologically and immunohistochemically for the presence of T. gondii tissue cysts. Results: Infected mice had low levels of anxiety, they have entered the open arms more frequently and have spent more time in the open arms of the maze compared to controls. Pathologically, in the infected group, high rates of gliosis, perivascular cell infiltration, meningitis, and neuron necrosis were observed especially in the parietal and temporal lobes, cornu ammonis, amygdala, and thalamus compared to the other parts of the brain. A higher number of tissue cyst formations were positively correlated with the lesion severity in these parts of the brain. Conclusion: It can be suggested that, tissue cysts and neuropathological changes in chronically infected mice brains have direct concern with behavioral manipulation which results from low anxiety levels. (Archives of Neuropsychiatry 2012; 49: 139-144)
Açıklama
DINCEL, Gungor Cagdas/0000-0002-6985-3197; Terzi, Osman Safa/0000-0002-7877-8897
Anahtar Kelimeler
Toxoplasma gondii, mice, tissue cyst, behavior
Kaynak
Noropsikiyatri Arsivi-Archives Of Neuropsychiatry
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
49
Sayı
2
Künye
Koçak O. M., Atmaca H. T., Terzi O. S., Büyükkayaer S., Rezaki H. Ö., Uzunalioglu T., Cağdaş G. D., Bal E., Kul O. (2012). Deneysel kronik toksoplazmoz fare modeli: Beyin lezyonlarının davranış değişiklikleri ile ilişkilendirilmesi. Nöropsikiyatri Arşivi, 49(2), 139 - 144.
