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Öğe Caustic esophageal injury decreases the number of interstitial cells of Cajal in the rat esophagus(Tubitak Scientific & Technical Research Council Turkey, 2010) Soyer, Tutku; Ayva, Sebnem; Somuncu, Salih; Atasoy, Pinar; Kanmaz, Turan; Cakmak, MuratAim: To evaluate the effect of caustic esophageal injury (CEI) on the number of interstitial cells of Cajal (ICC) Materials and methods: Wistar rats were divided into 3 groups. control, acid, and alkali induced CEI CEI was performed by the injection of 1 mL of 10% hypochloric acid in the acid group, and 1 mL of 10% sodium hydroxide was injected into the alkali group. Distal esophageal segments were removed 24 h after injection CEI was graded and the number of ICC were investigated (CD-117 staining) The number of ICC was compared in groups and correlated with CEI grades Results: Typical histopathologic features of CEI were encountered in acid (mean grade 0 25 +/- 0.15), and in alkali (median 2, range. 1 25-3) groups The number of ICC was decreased in both the acid (mean 12 8 +/- 9.2) and alkali (median 2, range 0.0-4 0) groups with respect to the control group (mean 30 5 +/- 6 5) (P < 0 05) In addition, alkali group had a decreased number of ICC, when compared to the acid group (P < 0 05) Histopathologic grading showed an inverse correlation with number of ICC in both the acid (rs: 0 678) and alkali (rs 0.759) groups Conclusion: CEI decreases the number of ICC in the rat esophagus. Alkali caustics cause a higher grade of esophageal injury and a greater decrease in the number of ICC Motility disorders after caustic ingestion may be related to the decreased number of ICCÖğe Effect of phototherapy on growth factor levels in neonatal rat skin(W B Saunders Co-Elsevier Inc, 2011) Soyer, Tutku; Ayva, Sebnem; Aliefendioglu, Didem; Aktuna, Zuhal; Aslan, Mustafa Kemal; Senyucel, Mine Fedakar; Cakmak, MuratAim: Neonates undergoing surgery may receive phototherapy (PT) for the treatment of hyperbilirubinemia. Although the effects of PT on neonatal structures are well documented, the effect of PT on wound healing has not been previously evaluated. An experimental study was performed to evaluate the effect of PT on growth factor levels responsible for wound healing in neonatal rat skin. Materials and Methods: Eighteen Wistar newborn rats (7 +/- 2 g) were included in the study. Rats were randomized into 3 groups: control (CG), PT, and sham (SG) (n = 6). Both groups had 1-cm median dorsal skin incision. In CG, 1 x 1 cm of dorsal skin was sampled including the incised skin. The PT group received 5 banks of blue light (wave density, 30-40 mu w/cm(2) per nanometer; exposure distance, 45 cm). Phototherapy was started 24 hours after birth and exposed during light period (mean duration, 21 hours to 15 minutes perpendicular to 2 hour to 1.5 minutes). Sham group consisted of animals that received a bank of white light with same exposure distance and a total duration of 26 hours to 18 minutes +/- 3 hours to 9.1 minutes. After exposure, 1 x 1 cm dorsal skin samples were obtained from both PT and SG groups, including the median incision. The effect of PT was evaluated with the expressions of vascular endothelial growth factor (VEGF), its receptor (VEGF receptor), and transforming growth factor beta (TGF-beta) in endothelial vessels and fibroblasts of neonatal skin samples. Results: There was no significant difference between groups in VEGF receptor and transforming growth factor beta expressions. The VEGF levels in endothelial vessels were significantly decreased in PT and SG when compared with CG (P < .05). Conclusion: Vascular endothelial growth factor is a mediator of angiogenesis and may decrease in neonatal rat skin after light exposure. It can be suggested that decreased levels of VEGF after PT application may alter angiogenesis and also may adversely affect the healing features of neonatal skin. (C) 2011 Elsevier Inc. All rights reserved.Öğe The effect of platelet rich fibrin on growth factor levels in urethral repair(W B Saunders Co-Elsevier Inc, 2013) Soyer, Tutku; Ayva, Sebnem; Boybeyi, Ozlem; Aslan, Mustafa Kemal; Cakmak, MuratAim: Platelet rich fibrin (PRF) is an autologous source of growth factors and promotes wound healing. An experimental study was performed to evaluate the effect of PRF on growth factor levels in urethral repair. Materials and Methods: Eighteen Wistar albino rats were included in the study. Rats were allocated in three groups (n:6): control (CG), sham (SG), and PRF (PRFG). In SG, a 5 mm vertical incision was performed in the penile urethra and repaired with 10/0 Vicryl (R) under a microscope. In PRFG, during the urethral repair as described in SG, 1 cc of blood was sampled from each rat and centrifuged for 10 minutes at 2400 rpm. PRF obtained from the centrifugation was placed on the repair site during closure. Penile urethras were sampled 24 hours after PRF application in PRFG and after urethral repair in SG. Transforming growth factor beta receptor (TGF-beta-R-CD105), vascular endothelial growth factor (VEGF) and its receptor (VEGF-R), as well as endothelial growth factor receptor (EGFR), were evaluated in subepithelia of the penile skin and urethra. Groups were compared for growth factor levels and growth factor receptor expression with the Kruskal Wallis test. Results: TGF-beta-R levels were significantly decreased in SG when compared to CG (p < 0.05). In PRFG, TGF-beta-R was increased in both subepithelia of penile skin and urethra with respect to SG (p < 0.05). When VEGF levels and its receptor expression were compared between SG and PRFG, VEGF levels were found to be increased in penile skin subepithelium, whereas VEGF-R expressions were decreased in urethral subepithelia in PRFG (p < 0.05). There was no difference between groups for EGFR levels (p > 0.05). Conclusion: Use of PRF after urethral repair increases TGF-beta-R and VEGF expressions in urethral tissue. PRF can be considered as an alternative measure to improve the success of urethral repair. (C) 2013 Elsevier Inc. All rights reserved.Öğe Evaluation of omental inflammatory response with P-/E-selectin levels and histopathologic findings in experimental model(W B Saunders Co-Elsevier Inc, 2012) Aslan, Mustafa Kemal; Boybeyi, Ozlem; Soyer, Tutku; Senyucel, Mine Fedakar; Ayva, Sebnem; Kisa, Ucler; Cakmak, MuratAim: The omentum plays a crucial role in abdominal defense mechanism by adhering to sites of inflammation and absorbing bacteria and debris from the peritoneal cavity. An experimental study was conducted to evaluate the inflammatory response of omentum in different abdominal events with omental P-/E-selectin levels and histopathologic findings. Materials and methods: Thirty Wistar rats were placed into 5 groups (n = 6), including a control group (CG), sham group (SG), bladder perforation (BP) group, splenic laceration (SL) group, and cecal ligation and puncture (CLP) group. Omental samples were obtained in CG after median laparotomy. In accordance with described models, BP, SL, and CLP were performed in experimental groups. Twenty-four hours after the first laparotomy, localization of the omental pad was noted, and omental samples were obtained for biochemical analysis of levels and histopathologic findings (no. of vessels in sections, polymorphic nuclear leukocytes [PMLs], lymphocytes). The mean P-/E-selectin levels and histopathologic findings of inflammation were compared between groups. Results: Although omentum was adhered to the cecum in all subjects after CLP, similar findings were not detected in other groups. P-selectin and E-selectin levels and number of PML were significantly increased in the CLP group when compared with other groups (P < .05). The number of vessels in sections was significantly increased in CLP group when compared with SG and BP groups (P < .05), and the BP group had a decreased number of vessels than CG (P < .05). The number of PML was significantly increased in SG and SL and BP groups with respect to CG (P < .05). Conclusion: Among different experimental intraabdominal catastrophes, only CLP caused an inflammatory response and increased levels of adhesion molecules in the omentum. These findings suggest that the nature of the inflammation is the main determining factor for the omental function in intraabdominal events. (c) 2012 Elsevier Inc. All rights reserved.Öğe Intrascrotal Extratesticular Neurofibroma as a Possible Cause of Failed Descent in Ipsilateral Testis(All India Inst Medical Sciences, 2012) Soyer, Tutku; Vargel, Ibrahim; Ayva, Sebnem; Cavusoglu, Tarik; Cesur, Ozkan; Bulbul, Selda; Cakmak, MuratIntrascrotal extratesticular neurofibromas (IEN) often originate from genitofemoral nerve (GFN) and present as a paratesticular mass. Synchronous presence of IEN and undescended testis has not been reported previously. A 12-year-old boy with neurocutaneous syndrome and congenital giant melanocytic nevi along with IEN and ipsilateral undescended testis is presented, to discuss the underlying pathophysiology of failed testicular descent in the presence of IEN.Öğe Myofibroblastoma of Breast in a Male Infant(Taylor & Francis Inc, 2012) Soyer, Tutku; Ayva, Sebnem; Senyucel, Mine F.; Senyucel, Cagri; Aslan, Mustafa K.; Cakmak, MuratBreast masses are very rare in infants and premature telarche due to excessive endogen or exogenous estrogens is the most common presentation during infancy. Myofibroblastoma is a stromal tumor of the breast, occurring especially in elder males. This breast mass has a close relationship between androgen receptors and has not been reported in infants previously. A 10-month old male baby with a left breast mass, which is diagnosed as myofibroblastoma, is discussed to evaluate the clinical features and treatment modalities of breast myofibroblastomas in children.Öğe Protective effect of selenium on intussusception-induced ischemia/reperfusion intestinal oxidative injury in rats(Tubitak Scientific & Technical Research Council Turkey, 2010) Akman, Hulya; Somuncu, Salih; Dikmen, Gunnur; Ayva, Sebnem; Soyer, Tutku; Dogan, Pakize; Cakmak, MuratAim: To evaluate the protective effect of selenium on intestinal ischemia-reperfusion injury due to intussusception. Materials and methods: Forty Wistar albino rats were allocated to 4 equal groups (n = 10) After anesthetization, 2 cm of intestinal segment, 20 cm proximal to the ileocecal valve, was removed in the control group (CG) In the intussusception group (ING), intussusception was performed 20 cm proximal to the ileocecal valve by pushing the proximal intestinal segment distally with a stylet, and 2 cm of intestinal segment was removed 4 h later Intussusception was reduced and a 2 cm segment was removed after another 4 h period in the intussusception-reduction group (IRG). The selenium group (SG) received 0.2 mg kg(-1) of selenium in 10 mL of 0 9% NaCl solution intraperitoneally 20 min before reduction and segments were removed 411 thereafter Intestinal tissues were evaluated for histopathological grades and oxidative injury markers Results: Histopathologic evaluations among the 4 groups did not reveal a significant difference (P > 0 05) Although catalase activity was decreased in all groups, increased activity was found in SG compared with IRG (P < 0 05) GSH-Px and SOD activities were also found to have decreased in ING and IRG (P < 0 05) CG and SG had no difference in GSH-Px and SOD activities (P > 0 05) Malondialdehyde (MDA) activities did not differ between CG and SG, but the MDA level was Increased in ING and IRG when compared with CG (P < 0 05) Conclusion: Although selenium prevents oxidative injury by increasing the antioxidant enzyme activity in experimental intussusception, minim effects on histopathologic findings were not detectedÖğe Rathke Cleft Cyst in Seven Year-old Girl Presenting with Central Diabetes Insipidus and Review of Literature(Freund Publishing House Ltd, 2010) Evliyaoglu, Olcay; Evliyaoglu, Çetin; Ayva, SebnemRathke cleft cysts (RCC) are benign cysts derived from remnants of Rathke cleft, and are rarely symptomatic in children. Symptoms due to RCC are associated with mass effect and pituitary hormone deficiencies. Slow growth rate of the cyst makes its incidence increase with aging. Here we report on a seven year old girl who presented with central diabetes insipidus (CDI). Her sella MRI revealed a lesion in the sellar region which grew rapidly in follow-up. She underwent microneurosurgical operation and the lesion was totally excised. Pathologic examination revealed RCC with degenerative changes. In her follow-up, growth hormone deficiency developed in addition to arginine vasopressin deficiency. Rapid growth of the cyst is not the usual course of RCC's. Mechanisms regarding the cyst growth are unclear as they are in this case. This is the youngest child to date presenting with central diabetes insipidus due to RCC. Rapid growth of RCC can cause CDI in young children.Öğe Tangier disease in a Turkish family(Wiley-Blackwell, 2014) Sahiner, Neriman; Kocak, Mukadder; Demirceken, Fulya; Kisa, Ucler; Ayva, Sebnem; Kazkayasi, MustafaIn this report we describe the upper gastrointestinal tractus involvement in a rare genetic disease of lipid metabolism. A 12-year-old boy presented with sore throat and fever. On physical examination, orange-yellow tonsils and adenoid tissue were noted. Mild hepatosplenomegaly was present. Lipid profile was compatible with Tangier disease (TD). Endoscopy of the upper gastrointestinal tract showed white-yellowish fatty deposits on the gastric mucosa. Microscopically, biopsy specimens contained numerous histiocytes with a foamy cytoplasm packed in the lamina propria of the gastric mucosa and at the crypt basement of the duodenum. His sister, 8 years old, was also diagnosed with TD based on abnormal lipid profile and orange-yellow tonsils. TD is a rare familial disorder of lipid metabolism, characterized by deposition of cholesteryl esters, probably involving the entirety of the gastrointestinal tract from the mouth to the anus.Öğe Tolerance of prefabricated skin flaps to ischaemia and reperfusion: Experimental study(Elsevier Sci Ltd, 2011) Serel, Savas; Cavusoglu, Tarik; Uluc, Arsin; Ayva, Sebnem; Saray, Aydin; Ozbek, Mustafa RizaCustom prefabrication of tissues allows the surgeon to build what is required for the reconstruction and has enabled the surgeon to reinforce new blood supply into selected blocks of tissue without vessel anastomosis. However, prefabricated flaps have several drawbacks and characteristics that differentiate them from conventional flaps. The objective of this study was to test the tolerance of prefabricated flaps to ischaemia/reperfusion injury in rats. In the first stage, the unilateral-inferior-epigastric pedicle was ligated and divided, and then a skin flap was fabricated by implantation of distally ligated femoral arteriovenous pedicle beneath the abdominal skin. The femoral vessels were implanted either in skeletonised or in muscle-cuffed fashion beneath the abdominal skin, a portion of which was raised as an island flap, based on these vessels. Prefabricated flaps (3 x 6 cm) were raised 6 weeks after, and were subjected to 10 h ischaemia and followed by 12 h reperfusion. Flap survival and histological changes at the pedicle-skin junction were evaluated at 7 days. Flap necrosis in the sham group was 0%, whereas the control group (conventional) had 47.27 +/- 13.50% necrosis. Flaps prefabricated with skeletonised femoral pedicle demonstrated an insignificant pattern with 63.74 +/- 10.62% necrosis when compared with prefabricated flaps with musclecuffed pedicle with the percentage of necrosis of 64.51 +/- 11.24. The area of necrosis was significantly increased when both the prefabricated flaps were compared with the control group or with the sham-prefabricated group (p < 0.05). Skin flaps prefabricated with either pedicle-alone or pedicles with muscle cuff are more susceptible to ischaemia and following reperfusion in comparison with the normal flaps. (C) 2010 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.
