Yazar "Bayraktaroglu, Alev Gurol" seçeneğine göre listele

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    Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats
    (Scientific Technical Research Council Turkey-Tubitak, 2015) Bayraktaroglu, Alev Gurol; Simsek, Ozkan; Kurum, Aytul; Arikan, Sevket; Ergun, Emel
    This study aimed to determine the alpha-naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats. In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study. Optimum reaction was achieved after a 3 h incubation period at pH 5.8, by ANAE staining. Two types of reaction were observed in ANAE (+) T lymphocytes. The first reaction was a dot-like positivity pattern characterized by the presence of large granules, while the other was a granular positivity pattern characterized by the presence of 3 to 5 small granules. B lymphocytes reacted negatively in ANAE staining. The rate of ANAE (+) lymphocytes in pregnant, kitten, and adult Angora cats was 62.89 +/- 1.29%, 68.37 +/- 1.22%, and 77.71 +/- 1.63%, respectively. In conclusion, the lowest rate of ANAE-positive lymphocytes was detected in the pregnant cats, depending on the maternal immune tolerance supported by hormonal mechanisms. An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system.
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    The structural properties of vimentin positive cells in the ileum epithelium of Angora rabbit
    (Ankara Univ Press, 2010) Beyaz, Feyzullah; Ergun, Emel; Ergun, Levent; Bayraktaroglu, Alev Gurol
    The study was projected to determine immunohistochemically vimentin positive cells by using anti-vimentin primary antibody, to eludicate ultrastructurally the morphological properties of these cells and, to examined the presence of villous M cells in villi epithelia. In this study, ten healthy, adult Angora rabbits were used as a material. It was examined the ileum with and without Peyer's patches. Immunohistochemical examination revealed the mature and immature M cells within FAE's and cup cells within epithelium of villi exibitcd the positive vimentin immunoreactivity. Electron microscopical examination revealed the mature M cells surrounded the intraepithelial lymphocytes (IEL) by a basolateral invagination. Whereas, immature M cells and cup cells were observed to be associated with one or two intraepithelial lymphocytes without such a pocketing. No villous M cells were found within epithelia of ileal villi. In conclusion, the cup cells are similar to immature M cells associated with IEL without a intraepithelial pocketing and showed a vimentin positive immunoreaction within their cytoplasms, but these cells are not villous M cells or enterocytes.