Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats
Yükleniyor...
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Scientific Technical Research Council Turkey-Tubitak
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study aimed to determine the alpha-naphthyl acetate esterase (ANAE) activity of peripheral blood leukocytes in pregnant, kitten, and adult Angora cats. In each group 9 healthy pregnant, adult, and kitten Angora cats constituted the material of the study. Optimum reaction was achieved after a 3 h incubation period at pH 5.8, by ANAE staining. Two types of reaction were observed in ANAE (+) T lymphocytes. The first reaction was a dot-like positivity pattern characterized by the presence of large granules, while the other was a granular positivity pattern characterized by the presence of 3 to 5 small granules. B lymphocytes reacted negatively in ANAE staining. The rate of ANAE (+) lymphocytes in pregnant, kitten, and adult Angora cats was 62.89 +/- 1.29%, 68.37 +/- 1.22%, and 77.71 +/- 1.63%, respectively. In conclusion, the lowest rate of ANAE-positive lymphocytes was detected in the pregnant cats, depending on the maternal immune tolerance supported by hormonal mechanisms. An increased rate of ANAE-positive lymphocytes was detected in the kittens, and the highest rate was measured in the adults, in parallel with the development of the immune system.
Açıklama
Anahtar Kelimeler
ANAE activity, Angora cats, lymphocyte, pregnancy
Kaynak
Turkish Journal Of Veterinary & Animal Sciences
WoS Q DeÄŸeri
Q4
Scopus Q DeÄŸeri
Cilt
39
Sayı
1
Künye
Bayraktaroğlu, Alev Gürol; Şimşek, Özkan; Kürüm, Aytül; Arıkan, Şevket; and ERGÜN, EMEL (2015) "Determination of alpha-naphthyl acetate esterase (ANAE) activity in peripheral blood leukocytes of pregnant, adult, and kitten Angora cats," Turkish Journal of Veterinary & Animal Sciences: 39(1), 57-61.
