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    Molecular Analysis of Three Local Silkworm Breeds (Alaca, Bursa Beyazi and Hatay Sarisi) by RAPD-PCR and SDS-PAGE Methods
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Akkir, Demet Eroglu; Yildiran, F. Azize Budak; Cakir, Suekran
    In this study, PCR-based RAPD method was used to determine the genetic variation in three Turkish local silkworm breeds (Alaca, Bursa Beyazi and Hatay Sarisi) and SDS-PAGE method was used to analyse the sericin proteins of their cocoons. Three local breeds were analysed by randomly chosen 40 primers and 68 total RAPD bands observed with 7 of them. Percentages of polymorphic loci were determined higher in Hatay Sarisi (55.88), lower in Bursa Beyazi and Alaca (44.12). Nei's genetic distance was determined 0.0637 between Bursa Beyazi and Alaca, 0.1012 between Bursa Beyazi and Hatay Sarisi, 0.0793 between Alaca and Hatay Sarisi. In SDS-PAGE sericin proteins of three breeds was yielded a single band of 200 kDa.
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    The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kirikkale Region
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Yildiran, F. Azize Budak; Yildiz, Kader; Cakir, Suekran; Gazyagci, Aycan N.
    In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCR using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, OPB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain.