The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kirikkale Region
Yükleniyor...
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kafkas Univ, Veteriner Fakultesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCR using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, OPB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain.
Açıklama
YILDIZ, Kader/0000-0001-5802-6156; YILDIRAN, Fatma Azize BUDAK/0000-0001-7031-6834
Anahtar Kelimeler
Echinococcus granulosus, Hydatid cyst, Polymorphism, RAPD-PCR
Kaynak
Kafkas Universitesi Veteriner Fakultesi Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
16
Sayı
2
Künye
Yıldıran F, Yıldız K, Çakır Ş, Gazyağcı A. (2010). The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kirikkale Region. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(2), 245 - 250.
