Yazar "Demir, Derya" seçeneğine göre listele

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    Immunohistochemical Evaluation of Apoptosis and Multidrug Resistance- Related Markers in Gallbladder Dysplasia and Carcinoma
    (2022) Başak, Kayhan; Demir, Derya; Koçdoğan, Arzu Kaya; Oğuztüzün, Serpil
    Objective: The search for treatment success in gallbladder carcinomas, which is one of the tumors with the most aggressive course, poor prognosis, and tendency to show resistance to treatment, continues today. Treatments targeting pathways related to genetic changes de- tected in most solid tumors offer new hope in the treatment of these tumors. Some of these treatment modalities target apoptosis-related pathways, and mammalian target of rapamycin (mTOR), p38, Bcl-2, and caspase-3 are important components of this pathway. Methods: In the study, mTOR, caspase-3, p38, Bcl-2, LL-37, MDR1, multidrug resistance protein (MRP)1, MRP6, and MRP7 immunohistochemical staining were applied to paraffin blocks of 27 gallbladder cancer and 62 cases with gallbladder dysplasia. The immunohisto- chemically stained sections were evaluated and scored. Results: mTOR, p38, and caspase-3 expressions were found to be significantly increased in dysplasia and tumor groups, and in dysplastic and malignant cells. While there was no signifi- cant difference in the expression of MRP1 and MRP7, MRP6 was significantly overexpressed. Conclusion: In this study, increased expression of mTOR, p38, and caspase-3 in the dys- plastic and malignant cells of the gallbladder may show that it has a role in the carcinogenesis process in the gallbladder. The study also shows that MRP6 may also play a role in the devel- opment of drug resistance in gallbladder carcinoma.
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    Subepithelial Fibrinous Accumulation and Associated Epithelial Proliferation in Laryngeal Nodules
    (2022) Başak, Kayhan; Günhan, Ömer; Çaputcu, Merve; Arda, Şule Sağlam; Atlı, Muharrem; Demir, Derya; Oğuztüzün, Serpil
    Objective: Fibrinoid accumulation in the larynx and increase in the subepithelial collage-\rnous connective tissue result in overgrowth. Mucosal epithelium may proliferate downward\rto organize and remove the fibrinoid accumulation. This downward proliferation may cause\ran invasive cancer-like image. This study focused on the pathogenesis of the accumulation of\rfibrinoid substance and the development mechanism of the associated squamous epithelium\rproliferation.\rMethods: Five hundred and seventy-five laryngeal nodules were reexamined and 111 of\rthem with varying degrees of irregular downward squamous epithelial proliferation were\rincluded in the study. Immunohistochemical staining of CK5/6, CK17, CK14, collagen type\rI, collagen type III, collagen type IV, and fibrinogen was performed. A modified Masson’s\rtrichrome method was used for the histochemical staining of collagen.\rResults: Edema was present in 18% of the acute lesions and fibrin deposition in 42%. Rela-\rtively mature lesions mostly contain dense collagen fibers. The intensity of collagen type III\rwas inversely proportional to the amount of fibrin accumulation. Collagen type IV was found\rin the epithelial and vascular basement membranes. A decrease in fibrin staining intensity\rand the presence of collagen type I and type III indicated the replacement of fibrin with col-\rlagen. Basal-type keratins showed more pronounced staining in the regenerated areas of the\repithelium. As the laryngeal subepithelial fibrinoid accumulation was replaced with collagen,\rregression of the lesion became difficult.\rConclusion: Irregular squamous epithelial proliferation occurs independent of the stage of\rthe lesion. Although the etiology is different, the resulting lesions are histologically similar to\rthose seen in the ligneous mucosal disease