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Öğe An Investigation on the Dna Binding Activities of Melamine, Cyanuric Acid and Uric Acid(Editura Acad Romane, 2021) Şenol, Ali; Devrim, Alparslan Kadir; Sudagidan, Mert; Özalp, Veli CengizMelamine can be added to various foods such as milk, milk powder, baby food, pet, and livestock feed for cheating purposes due to its high nitrogen content. Regarding its usage in food products, there is a need to investigate its possible interactions with DNA. Thus, this study aimed to investigate the interactions of melamine and its metabolized products, cyanuric acid and uric acid with genomic DNA, isolated from eukaryotic (calf thymus) and prokaryotic (Staphylococcus aureus) sources. UV-absorbance spectrophotometry, fluorescence spectrophotometry, and agarose gel electrophoresis techniques were used to evaluate these interactions. The five different concentrations of melamine, cyanuric acid, and uric acid were incubated with fixed DNA concentration and it was determined that the test compounds interacted with the DNA molecules. The data obtained by UV-absorbance and fluorescence spectrophotometry techniques revealed an increase in wave peaks observed with the increasing substance concentration. After the obtained data of the aforementioned techniques were evaluated together, it was concluded that melamine, cyanuric acid, and uric acid bonded to the eukaryotic and prokaryotic genomic DNA materials via groove binding.Öğe COMPARISON OF SOME COPPER COMPOUNDS IN TERMS OF THEIR INTERACTIONS WITH DNA USING AGAROSE GEL ELECTROPHORESIS, UV-ABSORBANCE AND FLUORESCENCE SPECTROPHOTOMETRY TECHNIQUES(Malaysian Society for Biochemistry and Molecular Biology, 2023) Akanbong, Elisha Apatewen; Devrim, Alparslan Kadir; Şenol, Ali; Sudagidan, MertIn this study, to investigate the interaction of genomic DNA with some copper compounds (CuSO4, CuCO3, and CuCl2) at various concentrations (1000 ?M, 500 ?M, 250 ?M, 125 ?M and 62.5 ?M); UV-absorbance spectrophotometry, agarose gel electrophoresis, and fluorescence spectrophotometry techniques were used. When the UV-spectrophotometry data were examined within the wavelength range of 220-320 nm, the hyperchromic effect of CuCl2 was evaluated to be proportional to its concentrations. Within the wavelength range, the copper compounds produced their strongest hyperchromic effect on the DNA at 1000 ?M. When the fluorescent spectrophotometry data were evaluated, the hypochromic effect of CuCl2 within the wavelength range of 400-700 nm was determined at 1000 ?M. CuSO4 showed a hypochromic effect at concentrations above 62.5 ?M. Also, it was observed that CuCO3 did not interact with DNA. According to the agarose gel electrophoresis findings, the copper compounds investigated decreased DNA band intensity as their concentrations increased. It was observed that the most significant decrease in band intensity was caused by CuCl2 at 1000 ?M. Again, it was observed that the copper compounds did not cause any cleavage in the genomic DNA within the concentration range 62.5-1000 ?M. Consequently, the copper compounds bind to DNA, most probably by non-intercalative mode. In this regard, they could have the potential to be used in the development of new therapeutic agents. Thence, conducting further studies on the interaction of the copper compounds with DNA, their possible DNA uncoiling activities, and also the investigation of these compounds in cancer cell lines will provide useful results. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.Öğe COMPREHENDING COVID-19: IMMUNOPATHOGENIC MECHANISMS OF CYTOKINE ACTION(2021) Akanbong, Elisha; Devrim, Alparslan Kadir; Şenol, Ali; Devrim, TubaCytokine release syndrome (CRS) or cytokine storm is as a result of the excess production of pro-inflammatory cytokines which is due to the disproportionate response of the immune system which can be instigated by SARS-CoV-2. COVID-19 which is caused by SARSCoV- 2 has a correlation with cytokines. SARSCoV- 2 instigates the production of IL-1 by ubiquitous macrophages and mast cells. IL-1 tends to influence the production of IL-6 and TNF-?. The progression of COVID-19 severity influences the level of certain cytokines such as IL-6. IL-6 is the cytokine chiefly responsible for the occurrence of CRS. CRS is the cause of COVID-19-related complications and the main cause of COVID-19-related deaths. Despite the data reported in the literature so far, the relationship between SARS-CoV-2 and cytokines has not been fully elucidated. The aim of the present review is to examine the relationship in question. Targeting cytokines during COVID-19 treatment has the potential to increase patient survival and reduce COVID-19-related deaths. It is concluded that focusing on the mechanisms of cytokine release and the effects of released cytokines, especially examining the effects on T lymphocytes and IFN-? production in COVID-19 disease, may help reduce the lethal effects of the disease.Öğe The concomitant use of USP28 and p53 to predict the progression of urothelial carcinoma of the bladder(ELSEVIER GMBH, 2020) Devrim, Tuba; Atac, Fatih; Devrim, Alparslan Kadir; Balci, MahiThe Ubiquitin Specific Peptidase 28 (USP28) is a deubiquitinase involved in the DNA damage pathway. Recently, USP28 protein is reported to play roles in the mechanism of p53 action and could be a possible prognostic marker for bladder cancer (BCa). This study aims to explore the relation of USP28 with tumor growth and invasion, and also to investigate the interplay between USP28 and p53 in BCa. Expression levels of USP28 and p53 in human BCa (invasive and non-invasive, n = 43) and control tissues (n = 8) were evaluated by immunohistochemistry and quantitative real-time polymerase chain reaction (qPCR) profiling. The relationship between protein and gene expression levels, clinicopathologic features and prognosis were evaluated. Significant positive correlations were found between BCa tumor progression and the USP28 expression, and also between the USP28 and p53 scores (p all < 0.05). Immunohistochemistry staining percentages were strongly correlated between USP28 and p53, and also positive correlations between tumor progression and p53 expressions were determined (p all < 0.001). Interestingly, USP28 and p53 were highly expressed and correlated in BCa. Consequently, the immunohistochemistry and qPCR results reported in our study suggested the idea that USP28 in coordination with p53 could serve as a marker in BCa progression.Öğe DNA-METAL INTERACTION AND THE BIOLOGICAL ACTIVITIES OF METAL COMPLEXES: AN OVERVIEW IN THE LIGHT OF RECENT LITERATURE(Editura Acad Romane, 2021) Senol, Ali; Akanbong, Elisha Apatewen; Sudagidan, Mert; Devrim, Alparslan KadirDeoxyribonucleic acid (DNA), which leads the biological synthesis of proteins and enzymes in living cells and carries genetic information, is a potential target for binding many molecules such as metals, metal complexes, anticancer drugs, and some harmful chemicals. Molecules that bind to DNA by covalent or non-covalent intercalation, electrostatic interaction, and groove binding cause disruption in the double-stranded DNA structure. This situation is of great importance in studies conducted in the field of pharmacy to increase the effectiveness of antitumor and antiviral drugs, in DNA-based disease diagnosis and determination of toxicity properties of metal complexes. The purpose of this review is to evaluate the interactions of DNA-metal complexes and provide information about their determined biological activities.Öğe Gastric Helicobacter-like Organisms in Stray Cats: Identification, Prevalence, and Pathologic Association(Univ Agriculture, Fac Veterinary Science, 2016) Dag, Serpil; Sozmen, Mahmut; Cihan, Mete; Tunca, Recai; Kurt, Basak; Devrim, Alparslan Kadir; Ozen, HasanTotal of 30 domestic stray cats (Felis cattus) were investigated for the presence of different species of gastric Helicobacter-like organisms (GHLO) by immunohistochemistry and polymerase chain reaction (PCR) analysis. The severity and distribution of pathologic changes in different regions of stomach were assessed microscopically. GHLO were present in all areas of the stomach in 93.3% cats investigated. Morphologically two different types of spiral bacteria were recognized. In 53.3% cats H. felis like organisms and in 76.7% cats H. heilmannii like organisms were determined. Mixed presence of both bacteria was seen in 43.3% cases. H. pylori was not detected in any of the cats. Mild to severe gastritis were observed in 90.0% cats. GHLO were present in fundus, corpus and pyloric antrum regions in similar densities. The most striking histopathological changes were lymphocyte and neutrophil infiltrations, fibrosis in the lamina propria, and lymphoid follicle formation. There was no significant relationship between the degree of bacterial density and the extent of histopathological changes. GHLOs were present on the mucosal surface, in the lumen of gastric glands, and in the cytoplasm of parietal cells. In conclusion, PCR and immunohistochemistry can be successfully used in detection of GHLOs. The results of the study show also that H. heilmannii and H. felis are frequent agents in stray cats, and hence suggest that these animals might be common reservoirs for these microorganisms. However, the bacteria do not seem to be solely responsible for gastritis observed in some stray cats. (C) 2015 PVJ. All rights reservedÖğe Protective effects of silymarin on fumonisin B-1-induced hepatotoxicity in mice(Korean Soc Veterinary Science, 2014) Sozmen, Mahmut; Devrim, Alparslan Kadir; Tunca, Recai; Bayezit, Murat; Dag, Serpil; Essiz, DincThe present study was conducted to investigate the effect of silymarin on experimental liver toxication induced by Fumonisin B-1 (FB1) in BALB/c mice. The mice were divided into six groups (n = 15). Group 1 served as the control. Group 2 was the silymarin control (100 mg/kg by gavage). Groups 3 and 4 were treated with FB1 (Group 3, 1.5 mg/kg FB1, intraperitoneally; and Group 4, 4.5 mg/kg FB1). Group 5 received FB1 (1.5 mg/kg) and silymarin (100 mg/kg), and Group 6 was given a higher dose of FB1 (4.5 mg/kg FB1) with silymarin (100 mg/kg). Silymarin treatment significantly decreased (p < 0.0001) the apoptotic rate. FB1 administration significantly increased (p < 0.0001) proliferating cell nuclear antigen and Ki-67 expression. Furthermore, FB1 elevated the levels of caspase-8 and tumor necrosis factor-alpha mediators while silymarin significantly reduced (p < 0.0001) the expression of these factors. Vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) expressions were significantly elevated in Group 4 (p < 0.0001). Silymarin administration alleviated increased VEGF and FGF-2 expression levels (p < 0.0001). In conclusion, silymarin ameliorated toxic liver damage caused by FB1 in BALB/c mice.Öğe Punch biyopsi ile deri yarası oluşturulan diyabetli ratlarda fosfodiesteraz-5 inhibisyonunun yara iyileşmesi üzerine etkileinin araştırılması(2015) Devrim, Alparslan Kadir; Değim, Zelihagül; Sözmen, Mahmut; Eşsiz, Dinç[Abstract Not Available]Öğe The effect of krill oil on Wnt/ß-catenin signaling pathway in acetaminophen-induced acute liver injury in mice(De Gruyter Open Ltd, 2023) Şahin, Yaşar; Devrim, Alparslan Kadir; Alçiğir, Mehmet Eray; Şenol, Ali; Ekici, Hüsamettin; Devrim, Tuba; Sudağidan, MertObjectives: This study investigated the effect of krill oil (KO) on liver damage caused by acetaminophen (APAP). Methods: In the present study, the control and APAP groups were given distilled water by gavage for 14 days. In addition, the KO and APAP+KO groups were given 500 mg/kg krill oil by gavage for 14 days. At the end of 14 days, 0.9 % sodium chloride solution (saline solution) administration was applied intraperitoneally to the control and KO groups. Meanwhile, 220 mg/kg acetaminophen was administered to the APAP and APAP+KO groups. While some biochemical parameters in plasma were examined, some oxidative stress parameters in plasma and liver tissue were evaluated. Apoptotic and inflammatory responses of some primer sequences determined by quantitative Real-Time PCR (qPCR) in liver tissue. After histopathological examination of liver tissue, immunohistochemical analysis was performed with Wnt inhibitory factor-1 (Wif-1), beta-catenin (ß-Catenin), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Results: The Wif-1 positivity in hepatocytes increased significantly in the APAP group (5.29 ± 0.71) compared to the control (1.14 ±0.51), and KO (2.14 ± 0.55) groups (p<0.001). The 8-OHdG positivity in hepatocytes increased significantly in the APAP group (19.57 ± 0.58) compared to the control (0.43 ± 0.20), KO (3.57 ± 0.48), and APAP+KO (4.00 ± 2.53) groups (p<0.001).Conclusions: As a result, krill oil could be used as a nutritional supplement to protect the liver against acetaminophen-induced liver injury. © 2023 De Gruyter. All rights reserved.Öğe The effect of krill oil on Wnt/ß-catenin signaling pathway in acetaminopheninduced acute liver injury in mice(Walter De Gruyter Gmbh, 2023) Şahin, Yaşar; Devrim, Alparslan Kadir; Alcigir, Mehmet Eray; Şenol, Ali; Ekici, Hüsamettin; Devrim, Tuba; Sudagidan, MertObjectives: This study investigated the effect of krill oil (KO) on liver damage caused by acetaminophen (APAP). Methods: In the present study, the control and APAP groups were given distilled water by gavage for 14 days. In addition, the KO and APAP+KO groups were given 500 mg/kg krill oil by gavage for 14 days. At the end of 14 days, 0.9 % sodium chloride solution (saline solution) administration was applied intraperitoneally to the control and KO groups. Meanwhile, 220 mg/kg acetaminophen was administered to the APAP and APAP+KO groups. While some biochemical parameters in plasma were examined, some oxidative stress parameters in plasma and liver tissue were evaluated. Apoptotic and inflammatory responses of some primer sequences determined by quantitative Real-Time PCR (qPCR) in liver tissue. After histopathological examination of liver tissue, immunohistochemical analysis was performed with Wnt inhibitory factor-1 (Wif-1), beta-catenin (ss-Catenin), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Results: The Wif-1 positivity in hepatocytes increased significantly in the APAP group (5.29 +/- 0.71) compared to the control (1.14 +/- 0.51), and KO (2.14 +/- 0.55) groups (p<0.001). The 8-OHdG positivity in hepatocytes increased significantly in the APAP group (19.57 +/- 0.58) compared to the control (0.43 +/- 0.20), KO (3.57 +/- 0.48), and APAP+KO (4.00 +/- 2.53) groups (p<0.001). Conclusions: As a result, krill oil could be used as a nutritional supplement to protect the liver against acetaminopheninduced liver injury.Öğe The relationship of leptin and thyroid hormones with milk yield and some fertility parameters in Simmental dairy cows during transition period(Tubitak Scientific & Technological Research Council Turkey, 2021) Duru, Özkan; Devrim, Alparslan Kadir; Macun, Hasan Ceyhun; Duru, Sibel Yasa; Şenol, AliIn this study, transition period cows were used to test the hypothesis that serum levels of leptin and thyroid hormones would correlate the production efficiency such as milk yield, body condition score, and fertility in dairy cows raised under the same controlled conditions. The study comprised 37 Simmental cows, which were 3 years old and between 257-265 days of gestation. Serum levels of leptin, T3, T4, FT3, and FT4 hormones were measured in blood samples obtained from the animals, which were in the period of 3 weeks before and 3 weeks after delivery. Prepartum serum leptin levels were significantly (p < 0.05) lower in cows having increased milk production compared to the first lactation peak value. Also, postpartum serum FT3 levels were significantly (p < 0.05) lower in cows having milk yield of >= 30 kg. Leptin levels were found to be inversely related with milk production and fertility. FT3 levels exhibited a negative correlation with milk production and similarly T3 levels showed an inverse correlation with body condition score. The present study proposed that routine monitoring of leptin and thyroid hormones would be useful for the reproductive management of cows with breeding problems.
