The effect of krill oil on Wnt/ß-catenin signaling pathway in acetaminophen-induced acute liver injury in mice
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Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
De Gruyter Open Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objectives: This study investigated the effect of krill oil (KO) on liver damage caused by acetaminophen (APAP). Methods: In the present study, the control and APAP groups were given distilled water by gavage for 14 days. In addition, the KO and APAP+KO groups were given 500 mg/kg krill oil by gavage for 14 days. At the end of 14 days, 0.9 % sodium chloride solution (saline solution) administration was applied intraperitoneally to the control and KO groups. Meanwhile, 220 mg/kg acetaminophen was administered to the APAP and APAP+KO groups. While some biochemical parameters in plasma were examined, some oxidative stress parameters in plasma and liver tissue were evaluated. Apoptotic and inflammatory responses of some primer sequences determined by quantitative Real-Time PCR (qPCR) in liver tissue. After histopathological examination of liver tissue, immunohistochemical analysis was performed with Wnt inhibitory factor-1 (Wif-1), beta-catenin (ß-Catenin), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Results: The Wif-1 positivity in hepatocytes increased significantly in the APAP group (5.29 ± 0.71) compared to the control (1.14 ±0.51), and KO (2.14 ± 0.55) groups (p<0.001). The 8-OHdG positivity in hepatocytes increased significantly in the APAP group (19.57 ± 0.58) compared to the control (0.43 ± 0.20), KO (3.57 ± 0.48), and APAP+KO (4.00 ± 2.53) groups (p<0.001).Conclusions: As a result, krill oil could be used as a nutritional supplement to protect the liver against acetaminophen-induced liver injury. © 2023 De Gruyter. All rights reserved.
Açıklama
Anahtar Kelimeler
acetaminophen; acute liver injury; krill oil; oxidative stress; qPCR
Kaynak
Turkish Journal of Biochemistry
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
48
Sayı
3
