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Öğe The effect of krill oil on Wnt/ß-catenin signaling pathway in acetaminopheninduced acute liver injury in mice(Walter De Gruyter Gmbh, 2023) Şahin, Yaşar; Devrim, Alparslan Kadir; Alcigir, Mehmet Eray; Şenol, Ali; Ekici, Hüsamettin; Devrim, Tuba; Sudagidan, MertObjectives: This study investigated the effect of krill oil (KO) on liver damage caused by acetaminophen (APAP). Methods: In the present study, the control and APAP groups were given distilled water by gavage for 14 days. In addition, the KO and APAP+KO groups were given 500 mg/kg krill oil by gavage for 14 days. At the end of 14 days, 0.9 % sodium chloride solution (saline solution) administration was applied intraperitoneally to the control and KO groups. Meanwhile, 220 mg/kg acetaminophen was administered to the APAP and APAP+KO groups. While some biochemical parameters in plasma were examined, some oxidative stress parameters in plasma and liver tissue were evaluated. Apoptotic and inflammatory responses of some primer sequences determined by quantitative Real-Time PCR (qPCR) in liver tissue. After histopathological examination of liver tissue, immunohistochemical analysis was performed with Wnt inhibitory factor-1 (Wif-1), beta-catenin (ss-Catenin), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Results: The Wif-1 positivity in hepatocytes increased significantly in the APAP group (5.29 +/- 0.71) compared to the control (1.14 +/- 0.51), and KO (2.14 +/- 0.55) groups (p<0.001). The 8-OHdG positivity in hepatocytes increased significantly in the APAP group (19.57 +/- 0.58) compared to the control (0.43 +/- 0.20), KO (3.57 +/- 0.48), and APAP+KO (4.00 +/- 2.53) groups (p<0.001). Conclusions: As a result, krill oil could be used as a nutritional supplement to protect the liver against acetaminopheninduced liver injury.Öğe Cafeteria diet can cause systemic inflammation and oxidative damage in the various tissues(Ios Press, 2024) Tursun, Serkan; Şahin, Yaşar; Alcigir, Mehmet Eray; Çınar, Miyase; Karahan, İrfanBACKGROUND: Cafeteria diet (CAF) is a succesful tool for establishing animal obesity model. This study purposed to show immunoexpression and oxidant-antioxidant status in the various organ tissues of Wistar rats fed CAF. METHODS: Two groups (six rats per group) of Wistar albino rats were fed CAF and standard chow (control) diets for eight weeks. After experimental procedure, the animals were sacrificed. Intestine, stomach, liver, kidney, spleen, heart, lung, and pancreas tissues were sent to Pathology Department and inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-kappa B) and cannabinoid receptor-1 (CB-1) immunoexpressions were assessed. Also, plasma liver function tests and lipid levels were measured total oxidant capacity (TOC) and total antioxidant capacity (TAC) were evaluated in both plasma and liver tissue. RESULTS: The immunoexpressions of NF-kappa B, iNOS and CB-1 were higher in the experiment group for all tissues. TOC was significantly higher in the experiment group, for both plasma and liver tissue (p < 0.001 and p = 0.02). TAC was higher in the experiment group's plasma measurement (p = 0.02), while there was no difference in the liver tissue between experiment and control groups (p = 0.11). CONCLUSION: CAF-induced obesity may be related to increased immunoexpression and oxidative damage in the various organs. Systemic problems should be considered and these phenomenon should be studied more comprehensively.Öğe An Investigation of Heavy Metal Concentrations in the Sera of Cattle Grazed in Different Locations in the Kars Province of Türkiye(Springer, 2024) Tarhane, Ayşe Kanıcı; Aluç, Yaşar; Kızıltepe, Semistan; Ekici, HüsamettinThe aim of this study was to investigate the effects of exposure of cattle to low levels of environmental pollution on trace metal metabolism. The assessment of heavy metal concentrations in blood samples is essential to evaluate the potential effects of pollutants on cattle production and to measure the uptake of pollutants by animals. For this purpose, cattle raised in villages at varying distances to the center of the Kars province were sampled for blood, and heavy metal concentrations were measured in the extracted sera. In total 150 blood samples were collected from 4-to 5-year-old cattle from 15 regions. Sera were extracted from the blood samples from the selected foci and analyzed with an inductively coupled plasma-optical emission spectrometry (ICP-OES) device for essential (Co, Cr, Cu, Fe, Mn, Ni and Zn, Se, V) and non-essential (Pb, Cd and Hg, As, Al, Sn) heavy metals. Heavy metals were detected in the serum samples in the following order: Sn > Fe > Al > Zn > Cu > Pb > Cr > As > V > Ni > Mn > Hg. A confidence interval of 95% was used to evaluate all tests. Differences between the villages were insignificant for the Cu, Pb, Cd, Hg, Se, Al, Co concentrations (p > 0.05), whereas differences between the villages were significant for the Fe, As, Ni, Mn (p < 0.05), Zn, Cr (p < 0.01), V and Sn (p < 0.001) concentrations. The results of this study show that heavy metals detected in bovine sera from the sampling area do not exceed the maximum permissible limits, suggesting limited exposure to heavy metals and no associated health risk to animals in the region.Öğe Alginate-based bio-nanocomposite reinforced with poly(2-hydroxypropyl methacrylamide) and magnetite graphene oxide for delivery of etoposide and photothermal therapy(Elsevier Sci Ltd, 2024) Işıklan, Nuran; Geyik, Gülcan; Güncüm, EnesRecently, bio-nanocomposite nanogels/hydrogels composed of natural polymers and carbon-based nano- materials are attracting increasing attention in the fields of nanomedicine and bioengineering due to their unique properties. In this study, we propose the creation of an innovative multifunctional bio-nanocomposite based on alginate graft copolymer with poly(2-hydroxypropyl methacrylamide) (SA-g-PHPM) and magnetite graphene oxide (mGO) loaded with etoposide (EPS) for drug delivery and photothermal therapy (PTT). The SA-g-PHPM/ mGO bio-nanocomposite was synthesized using an emulsion method and exhibited favorable physicochemical properties. The structural functionalities and surface morphology of the SA-g-PHPM/mGO bio-nanocomposite were comprehensively characterized using spectroscopic techniques, including FT-IR, XRD, UV, DLS, TEM/FESEM, and AFM analyses. Under near-infrared (NIR) irradiation (808 nm, 1 Wcm-(2), 10 min), the SA-g-PHPM/ mGO bio-nanocomposite demonstrated the ability to effectively induce a temperature increase exceeding 29 degrees C. Additionally, exposure to NIR light and magnetic field led to an increased release of EPS whereas an increment in percentage of mGO caused a decreased EPS release. Notably, the synergistic effects of chemotherapy, light-triggered drug release, and PTT collectively contributed to a significant enhancement in lung cancer (H1299) cell death. In conclusion, our findings suggest that the developed SA-g-PHPM/mGO/EPS bionanocomposite serves as an efficient vehicle for multimodal therapy in lung cancer.Öğe Age-and sex-related changes in selected hematological parameters, lipid peroxidation and erythrocytes osmotic fragility of Turkish Angora cats(Hellenic Veterinary Medical Soc, 2022) Kabakcı, Ruhi; Yigit, A. A.; Kara, E.; Çınar, M.; Ekici, Hüsamettin; Aluç, YaşarThis study was conducted to investigate the changes in selected hematological parameters, lipid peroxidation and osmotic fragility of erythrocytes in Angora cats depending on age and gender. For this purpose, the blood samples were collected from vena saphena medialis of 9 young and 14 adult cats which were also classified as male (n=12) and female (n=11). Following hematological analysis, samples were washed with PBS by centrifugation and 10% hematocrit suspension was prepared from the erythrocytes pellet for the osmotic fragility test. The concentration of malondialdehyde (MDA) was also measured from lysed erythrocytes to determine lipid peroxidation level. Red blood cells (RBCs), hemoglobin, and hematocrit were significantly (P<0.001) high in adults while the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly (P<0.05) high in young cats. Erythrocyte MDA level was also higher statistically (P<0.05) in adult cats than in young cats. There was no significance (P>0.05) in these parameters between male and female cats. Findings of fragility tests showed that erythrocytes of young and male cats were statistically more susceptible to hypotonic NaCl solutions than those of adult and female cats, respectively (P<0.01, P<0.05). It was concluded that erythrocytes related parameters in Angora cats changed depending on age rather than gender except for stability of RBCs.Öğe Comparison of the Algan Hemostatic Agent with Floseal in Rat Liver Laceration Bleeding Model(Aves, 2022) Binnetoğlu, Kenan; Kumandaş, Ali; Ekici, Hüsamettin; Özbaykuş, Abdullah CanberkObjective: Major vascular injury is one of the most important causes of death after trauma. 'The effective and speedy control of the hemorrhage is crucial in reducing deaths. Many products are used for this purpose. Today, however, an ideal product has not yet been produced and there is a strong demand for such effective hemostatic products. The aim of this study is to compare the efficacy of Algan hemostatic agent with Floseal in the liver laceration model in rats. Materials and Methods: A total of 28 rats were used in the study. Rats were divided into 4 groups, each consisting of 7 rats. Experimental liver laceration was established. In the control group. saline-impregnated gauze was applied. Algan hemostatic agent-impregnated sponge, Algan hemostatic agent powder, and Floseal gel were applied to the experimental groups. Results: There was no difference in bleeding control among the Algan hemostatic agent powder. Algan hemostatic agent-impregnated sponge, and Floseal. When compared to the control group, Algan hemostatic agent powder, Algan hemostatic agent-impregnated sponge, and Floseal were found to be very effective in bleeding control, respectively (P = .001, .012, and .002), in the experimental groups. Conclusion: This study showed that the properties of both Algan hemostatic agent powder and Algan hemostatic agent-impregnated sponge for controlling bleeding are similar to Floseal. Considering other characteristics such as Algan hemostatic agent's naturalness, easy applicability, and low cost, Algan hemostatic agent has been a promising effective hemostatic agent.Öğe The apoptotic and proliferative effects of tulathromycin and gamithromycin on bovine tracheal epithelial cell culture(Tubitak Scientific & Technological Research Council Turkey, 2022) Şahin, Yaşar; Yıldırım, Ebru; Yurdakök Dikmen, Begüm; Türk, MustafaGamithromycin and tulathromycin are commonly used in the treatment of bovine respiratory bacterial diseases. The current work was undertaken to establish the apoptotic, necrotic, and cytotoxic effects of these antibiotics in the target animal. Cells with apoptosis and necrosis were determined by dual staining method, cytotoxic effects were determined by MTT assay, cell proliferative effects were examined by XCelligence real-time cell analysis system (RTCA-SP). The comparison between gamithromycin and tulathromycin concentrations on tracheal cells in terms of % cell viability was found to be significantly different. While the cell viability percentage of gamithromycin was higher at 150 mu g/mL, 180 mu g/mL, and 240 mu g/mL than tulathromycin, and at 2 mu g/mL, 4 mu g/mL, 10 mu g/mL, 20 mu g/mL, and 50 mu g/mL concentrations tulathromycin cell viability was higher than gamithromycin (p < 0.05). When the staining method data were evaluated, the difference between the results of % apoptotic index at 20 mu g/mL concentration was significant and it was found that gamithromycin had more apoptotic effect than tulathromycin (p < 0.05). It was seen that tulathromycin and gamithromycin applied on tracheal epithelial cells at concentrations of 2 and 10 mu g/mL increased the viability depending on time. The increase in epithelial cell proliferation of gamithromycin and tulathromycin due to time shows that these antibiotics can maintain longterm prophylactic treatment against diseases.Öğe Effectiveness of Algan Hemostatic Agent in Bleeding Control: An Experimental Kidney Incision Model(Galenos Yayincilik, 2021) Midi, Ahmet; Kumandaş, Ali; Ekici, Hüsamettin; Kılıç, Yusuf; Özgöçmen, RuchanAim: The purpose of this study is to evaluate the hemostatic efficacy of a novel plant originated blood stopper which is called Algan Hemostatic Agent (AHA) in uncontrolled kidney incision bleeding model. Methods: The study was conducted with thirty-two rats 5-7 weeks old. The rats were randomly divided into 4 groups, each consisting of eight rats (Group 1: heparinized saline, 2: heparinized AHA, 3: non-heparinized saline, 4: non-heparinized AHA). Experimental kidney incision was made, and saline soaked sponge was applied to the control group and AHA to the study group. Results: The duration of bleeding was significantly shorter in the AHA group compared to the control group. The AHA has controlled the bleeding in the heparinized and non-heparinized groups 5 +/- 1.19 and 3 +/- 0.91 seconds respectively (p<0.001). In the control group, hemorrhage was controlled in the heparinized and non-heparinized group 180.6 +/- 25.4 and 125.4 +/- 21 seconds respectively. Conclusion: This study demonstrated that the AHA is useful in controlling bleeding in the Rat kidney incision model.Öğe Investigation of the biocompatibility and in vivo wound healing effect of Cotinus coggygria extracts(Ankara Univ, 2024) Bozkaya, Esra; Türk, Mustafa; Ekici, Hüsamettin; Karahan, SiyamiCotinus coggygria is widely recognized its antiseptic, anti-inflammatory, antimicrobial, antihemorrhagic, and wound-healing properties. In this, aimed to evaluate the phenolic contents, cytotoxicity/proliferation, hemolytic, antimicrobial, genotoxic, apoptotic, necrotic activities, and in vivo wound healing effects of C. coggygria, , a plant species known to have beneficial effects on wound healing. TOF-LC/MS analyzes revealed that the methanol extract of C. coggygria leaves contained flavonoids and phenolic compounds such as gallic acid (18.5 mg/kg), catechin (4.6 mg/kg), protocatesic acid (0.6 mg/kg), vanillic acid (8.4 mg/kg), ellagic acid (0.1 mg/kg), rosmarinic acid (0.1 mg/kg), quercetin (15 ppb) and C. coggygria stems contained such as gallic acid (24.6 mg/kg), catechin (155.1 mg/kg), chlorogenic acid (1.9 mg/kg), 4hydroxybenzoic acid (383.3 mg/kg), rutin (2.5 mg/kg), ellagic acid (15.1 mg/kg), apigenin 7-glycoside (10.5 mg/kg), rosmarinic acid (0,4 mg/kg), quercetin (15.2 mg/kg), naringenin (279.1 mg/kg). Consequently, C. coggyria has a positive effect on wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, or hemolytic effects at test concentrations. In the in vivo burn model, wounds treated with leaf and stem extracts healed faster than the control group. Thus, C. coggygria is an effective plant for wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, and hemolytic effects.Öğe Determination of blood heavy metal concentrations and oxidant-antioxidant capacities in Angora cats at different age and gender(Ankara Univ, 2023) Kabakçı, Ruhi; Yiğit, Ayşe Arzu; Ekici, Hüsamettin; Aluç, Yaşar; Kara, ErdalThis study was conducted to investigate heavy metal concentrations and oxidative status of plasma and erythrocytes in Angora cats at different ages and gender. Sixteen young (less than 1 year old) and 14 adult (1-6 years old) cats were also grouped according to gender as male (n = 17) and female (n = 13). The separated plasma samples from cat's blood were analyzed for selected heavy metals and total oxidant and antioxidant capacities (TOC and TAC) and calculated for oxidative stress index (OSI). The erythrocyte hemolysates were also evaluated for malondialdehyde (MDA), and super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Results of the study showed that most of the measured metals were not varied statistically according to age or gender. However, adult cats had significantly (P<0.01) higher Cu and lower Fe levels compared to young cats. Plasma levels of TOC, TAC and OSI, and erythrocyte MDA concentrations in young cats were significantly (P<0.05) higher than that of adults. While the SOD activity was decreased by the age, GPX activity was increased (P<0.05). However, the activity of CAT was changed by only gender, which was higher in males (P<0.01). In conclusion, metals, especially trace elements, are required for many kinds of physiological processes and the synthesis of antioxidant enzymes. Therefore, it can be suggested that the periodic measurement of metals and the addition of common antioxidant supplements to the diet of adult Angora cats will support weakening antioxidant mechanisms by age.Öğe Effects of functional poly(ethylene terephthalate) nanofibers modified with sericin-capped silver nanoparticles on histopathological changes in parenchymal organs and oxidative stress in a rat burn wound model(Ankara Univ, 2023) Şahin, Yaşar; Gün Gök, Zehra; Alçığır, Mehmet Eray; Çınar, MiyasIn this study, it was aimed to investigate the effect of a poly(ethylene terephthalate)-g-poly(hydroxyethyl methacrylate) (PET-g-HEMA) nanofiber wound dressing modified with sericin-coated silver nanoparticles (S-AgNPs) on internal organs, oxidative stress, and biochemical parameters. To establish a burn model, the backs of anesthetized rats were shaved and then third-degree burns were created with a round-bottomed stainless steel rod 2 cm in diameter kept in 100 degrees C water for 20 seconds. The wounds of the negative control group (G1) were covered with standard bandages; the wounds of the positive control group (G2) were covered with silvercel, used as burn wound material; and the wounds of the experimental group (G3) were covered with PET-based dressing material. Histopathological changes in organs (liver, kidneys, heart, pancreas, lungs), total oxidant status (TOS), total antioxidant status (TAS), nitric oxide (NO), and biochemical parameters (serum aspartate aminotransferase [AST], alanine aminotransferase [ALT], gamma glutamyl transpeptidase [GGT], creatine kinase, lactate dehydrogenase [LDH], total protein, albumin, globulin, urea) were examined. Compared with the G1 group, plasma AST, ALT, and GGT levels were found to be significantly decreased in G2 and G3 (P<0.001). Plasma TAS was found to be significantly increased in G2 and G3 compared to G1 (P<0.05). Compared to the G1 group, degenerative and necrotic changes in the liver, kidneys, and pancreas were found to be significantly reduced in G2 and G3 (P<0.05). In conclusion, this work demonstrates that the synthesized PET-based wound dressing material has the capacity to be used commercially.Öğe Investigation of The Effectiveness of Plant Based Algan Hemostatic Agent in a Rat Model of Femoral Arterial Bleeding(Bezmialem Vakif Univ, 2022) Ekici, Hüsamettin; Kumandaş, Ali; Özbaykuş, Abdullah Canberk; Dizdaroglu, Hazal; Midi, Ahmet; Balik, Mehmet Sabri; Yesilada, ErdemObjective: The aim of this study is to evaluate the efficacy of the Algan Hemostatic Agent (AHA) available in three different physical form (liquid, powder and sponge absorbed) in the femoral artery incision model in rats. Methods: A total of sixty-four 5-7 weeks old rats were used in the study. Rats were randomly divided into 8 groups each consisting of eight rats (4 groups heparinized and 4 groups non-heparinized). An experimental femoral artery incision was created. As a control, physiological saline absorbed sponge was applied. AHA liquid, AHA powder and AHA sponge absorbed forms were applied to the experimental groups. Results: Upon application to the bleeding sites, all the AHA forms stopped bleeding in a significantly shorter time compared to the control group (p<0.05). In contrast, bleeding in control group could not be controlled within 4 minutes. The best result was in AHA powder form and it was able to control bleeding in the non-heparinized group at 87.5% in the first minute and 12.5% in the second minute. In the heparinize group, the AHA powder form was able to control the bleeding at 62.5% in the first minute and 37.5% in the second minute. Conclusion: This study shows that AHA is a highly effective and promising hemostatic agent in bleeding control.Öğe Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract(Ankara Univ, 2023) Bozkaya, Ogun; Ekici, Hüsamettin; Gün Gök, Zehra; Arat, Esra; Ekici, Seda; Yigitoglu, Mustafa; Vargel, İbrahimIn recent years, researchers have shown an increased interest in using medicinal plants for the synthesis of silver nanoparticles (AgNPs) having various therapeutic properties. Centella asiatica (CA), a medicinal plant, has been used to treat minor burn wounds, psoriasis, and hypertrophic wounds among many other pathological conditions. The current study aimed to synthesize CA coated AgNPs (CA-AgNPs) with appropriate biocompatibility and various therapeutic properties, including antimicrobial and analgesic activities. The synthesized CA-AgNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, zeta potential measurements, and fourier transform infrared (FT-IR) spectroscopy. The formation of spherical CA-AgNPs was confirmed by a single surface plasmon resonance (SPR) peak emerging at 420 nm wavelength by UV-Vis. The average hydrodynamic diameter and zeta potential of the particles were found to be 29.5 nm and -24.5 mV, respectively. The FT-IR analyses showed that the AgNPs were coated and stabilized by bioactive compounds from the CA extract. MTT cytotoxicity assay revealed that CA-AgNPs at <= 1 mM concentrations exhibited biocompatibility for L929 fibroblast cells. The antimicrobial activity of CA-AgNPs was confirmed by significant inhibition of Staphylococcus aureus and Escherichia coli. In addition, the analgesic effect of CA-AgNPs was investigated for the first time in the literature by tail-flick and hot plate methods, and statistically significant results were obtained for both methods. Taken together, these results suggest that CA-AgNPs can be used as an effective antibacterial and analgesic agent in a variety of biomedical applications, including coating wound dressings.Öğe Survey of aflatoxin residue in feed and milk samples in Kırıkkale province, Turkey(2018) Yıldırım, Ebru; Macun, Hasan Ceyhun; Yalçınkaya, İlkay; Kocasarı, Fatma Şahindokuyucu; Ekici, HüsamettinBu çalışmada Kırıkkale ilindeki sütçü inek işletmelerinden toplanan yem ve süt örneklerinde total aflatoksin (AF) ve aflatoxin M1 (AFM1) kontaminasyonuna bakıldı. Türkiye, Kırıkkale İlindeki ilçelerden (Delice, Keskin, Sulakyurt, Bahşılı, Yahşihan, Çelebi, Karakeçili, Balışeyh) ve merkezden Haziran 2012 - Ağustos 2013 tarihleri arasında 154 yem ve 154 süt örneği toplandı. Toplanan numunelerdeki total AF ve AFM1' in kantitatif analizi, ticari kitlerle (HELICA biosystems inc.) enzim bağlı immünoassay (ELISA) kullanılarak gerçekleştirildi. Tüm yem ve süt örneklerinde total AF ve AFMsaptanmıştır. 154 yem örneğinden 5'inde total AF miktarı 20 µg/kg'ın üstünde bulunmuştur. Süt örneklerinin hiçbiri yasal sınırı aşmamıştır. Süt yemlerinde toplam AF düzeyi minumum 0.20 µg/kg, maksimum 28.80 µg/kg olarak tespit edilmiştir ve ortalaması 6.43±7.01 µg/kg'dir. Süt örneklerinde AFM1düzeyi minimum 0.08 ng/L, maksimum 10.11 ng/L tespit edilmiştir ve ortalaması 1.73 ±2.20 ng/L'dir. Sonuç olarak, tüm süt örneklerinde AFM1 kalıntısı bulunsa da bu yasal düzeyin üstünde değildir. Yem örneklerinin ise sadece %3.25 kadarı 20 µg/kg üzerinde total AF içermektedir. Halk sağlığı yönünden AFM1 bir risk oluşturmamaktadır. Aflatoksin bulaşmalarının önüne geçmek için stratejiler geliştirilmeli ve izleme programları oluşturulmalıdırÖğe Kadmiyum, bakır ve kurşunun in vitro inek uterus kasılmaları üzerine etkileri(2013) Yıldırım, Ebru; Macun, Hasan CeyhunBu çalışma, in vitro koşullarda kadmiyum, bakır ve kurşunun folliküler ve luteal evredeki izole inek uterus kasılmaları üzerine olan etkilerinin belirlenmesi amacı ile yapıldı. Çalışmada; Kırıkkale mezbahasında kesilen sağlıklı Holstein ırkı ineklerin myometrial şeritleri kullanıldı. Bu şeritler, izole organ banyosuna 2 g ön gerimle asıldı. Spontan, 2.5 mIU/ml oksitosin ve 10-6 M prostaglandin F2 alfa (PGF2 alfa) ile oluşturulan kasılmalar üzerine 10-5 M kadmiyum, 10-4 M bakır ve 10-4 M kurşunun etkileri değerlendirildi. Kadmiyum, folliküler evrede oksitosin ve PGF2 alfa’nın, luteal evrede ise spontan, oksitosin ve PGF2 alfa’ nın oluşturduğu kasılmaların tüm parametrelerini (maksimum ve ortalama amplitüd, frekans değerleri) anlamlı olarak azalttı. Bakır; folliküler evrede spontan kasılmaların ortalama ve maksimum amplitüd değerlerini, oksitosin kasılmalarının tüm parametrelerini, PGF2 alfa kasılmalarının frekans ve maksimum amplitüd değerlerini düşürdü. Luteal evrede bakır, spontan kasılmaların maksimum amplitüdünü, oksitosin kasılmalarının frekans ve maksimum amplitüdünü, PGF2 alfa kasılmalarının ortalama ve maksimumum amplitüd değerlerini düşürdü. Kurşun, folliküler ve luteal evrede PGF2 alfa ve oksitosin kasılmalarının frekans değerini azaltırken, luteal evrede spontan kasılmaların ortalama amplitüd değerini artırdı. Sonuç olarak; kadmiyum, bakır ve kurşunun inek uterusunun kasılma yanıtlarını değiştirdiği ve buna bağlı olarak fertiliteyi etkileyebileceği kanısına varıldı.Öğe Healing Effects of Single-Dose Triptolide in Rats with Severe Acute Pancreatitis(2020) Özden, Hüseyin; Kilitci, Asuman; Şahin, Yaşar; Karaca, Gökhan; Umudum, Haldun; Yıldız, Ahmet; Dulkadiroğlu, ErkanAim: Severe acute pancreatitis (SAP) carries high morbidity and mortality risk. If the proinflammatory response phase of SAP cannot be controlled, it may result in multiorgan failure (MOF). Nuclear factor-kappa B (NF-?B) activation plays an important role in the development of MOF. In this study, it was aimed to investigate the healing effects of triptolide, an anti-inflammatory and immunosuppressive agent in rats with SAP. Material and Methods: A total of 20 Wistar-Albino rats were divided into two groups as the SAP and triptolide treatment (TT) groups. SAP was induced by intraperitoneal injection of cerulean (50 mg/kg) in both groups. TT group was administered a single dose (0.2 mg/kg) triptolide 24 hour after the induction of SAP. Serum ALT, AST, GGT, Lipase, Glucose, ALP and amylase levels and pancreatic tissue samples were examined. Results: Serum glucose and amylase levels were found to be significantly lower in the TT group (p=0.011 and p=0.035, respectively). There was no significant difference between the groups in terms of other biochemical parameters. Pancreatic edema, acinar cell degeneration, fat necrosis, intrapancreatic&perivascular inflammation, inflammation in the peripancreatic fat tissue were common histopathological findings in both groups. There was no significant difference between the groups in terms of histopathologic changes. Conclusion: Cerulein-induced pancreatitis is a successful method for experimental SAP. The healing effects of single-dose triptolide treatment are not evident in the early phase of SAP. The therapeutic effects of triptolide on inflammatory and oxidative stress were not significantly approved by histopathological and biochemical parameters by the pancreatic tissue.Öğe What is the protective effect of krill oil on rat ovary against ischemia–reperfusion injury?(Blackwell Publishing, 2019) Yeral I.; Sayan C.D.; Karaca G.; Simsek Y.; Sagsoz N.; Ozkan Z.S.; Atasoy P.Aim: In this study, we aimed to investigate the protective effect of krill oil (KO) against ischemia–reperfusion (I/R) injury on rat ovary. Methods: This study was conducted with 32 Wistar Albino rats. Rats were divided into four groups, with eight rats in each group-as follows: Sham group, I/R group, I/R + low dose KO group (50 mg) and I/R + high dose KO group (500 mg). The histopathological and follicle counts were performed on the right ovary. The total antioxidant status, total oxidant status and oxidative stress index were evaluated on the left ovary. And also serum N-thiol level, serum T-thiol level, serum disulfide (SDS) level, serum disulfide/N-thiol and serum disulfide/T-thiol ratios were evaluated too. Results: A statistically significant difference was determined between the I/R group and all the other groups for all parameters. There was significant difference between KO groups and the Sham group for the parameters of serum N-thiol, serum T-thiol, SDS, serum disulfide/N-thiol and serum disulfide/T-thiol. SDS, total oxidant status and oxidative stress index were determined to be the highest in the I/R group and the lowest in the low dose KO group. The total antioxidant status values were found to be the highest in the high dose KO group and the lowest in the I/R group. Follicle counts and histological injury scores showed no significant difference between Sham and KO groups. Conclusion: This study demonstrated that KO has beneficial effects on decreasing the injury after I/R on rat ovary. © 2018 Japan Society of Obstetrics and GynecologyÖğe In vitro exposure to di-(2-ethylhexyl) phthalate (DEHP) stimulates spontaneous feline uterine contractions(SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK, 2020) Kabakci, Ruhi; Macun, Hasan Ceyhun; Yildirim, Ebru; Polat, Ibrahim Mert; Elifoglu, Taha BurakDi-(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in various products such as PVC-derived plastics, toys, packaging materials, cosmetics, and pharmaceuticals. This study aimed to investigate the effect of DEHP on spontaneous contractions of the feline uterus in vitro. Tubal 1-cm uterine samples prepared from 10, 9, and 12 uteri obtained from adult cats in estrus (n = 5), diestrus (n = 5), and interestrus (n = 5), respectively, after ovariohysterectomy were suspended in an isolated organ bath in aerated Krebs solution at 39 +/- 1 degrees C, and an initial 1 g tension was given. After 1 h equilibration of tissues, the spontaneous contractions were recorded for 10 min as control. The effects of solvent and DEHP (0.001-100 mu M) on contractions were then evaluated in terms of frequency and mean amplitude parameters. It was observed that DEHP had no effect on uterine contractions of cats in interestrus. However, DEHP significantly increased the mean amplitude of uterine contractions during the estrus and diestrus periods at concentrations of 1 mu M and 10 mu M, respectively, depending on the dose (P < 0.05). Decreases in the frequency of the contractions in the estrus and diestrus periods were not statistically significant (P > 0.05). 'this study, carried out for the first time in cats, showed that DEHP has a stimulatory effect on uterine contractions. We concluded that disruption of the uterine contractions, which are essential for physiological reproductive processes such as regular estrous cycles, sperm and zygote transport, implantation and continuation of pregnancy, by DEHP exposure may cause many reproductive problems.Öğe Evaluation of melamine and cyanuric acid cytotoxicity: an in vitro study on L929 fibroblasts and CHO cell line(ANKARA UNIV PRESS, 2020) Melekoglu, Abdullah; Ekici, Husamettin; Arat, Esra; Karahan, SiyamiMelamine and its metabolites pose health concern as they are used in various industrial products including feed and drugs. There are a limited number of studies on melamine and cyanuric acid cytotoxicity and cellular damage without a certain conclusion. The present study aimed to evaluate melamine, cyanuric acid and its combined cytotoxic effects using 3-(4.5-dimethylthiazol-2-yl) methyl thiazole tetrazolium (MTT) bromide test. The study also evaluated apoptotic and necrotic effect using a double staining method of Hoechst 33342 and propidium iodide. Melamine, cyanuric acid and their combination (1:1) were applied to L929 fibroblasts and Chinese hamster ovary (CHO) cells at various concentrations (1000 mu g/mL, 500 mu g/mL, 250 mu g/mL, 125 mu g/mL and 62.5 mu g/mL). At the highest concentration (1000 mu g/mL), the cell viability dropped down approximately to 50% both in CHO cells and L929 cells. Melamine, cyanuric acid and their mixture caused cytotoxicity in CHO cells and L929 fibroblasts in dose-dependent manner Cell death occurred through both apoptosis and mainly necrosis. Both cell types were more sensitive to the mixture of melamine and cyanuric acid and, furthermore, CHO cells were more sensitive than L929 fibroblasts. As a result, melamine, cyanuric acid and their combination caused cytotoxicity in CHO cells and L929 fibroblasts. Further studies should be conducted in different cell lines. These studies should also aim to reveal the mechanism of cytotoxicity and related pathways.Öğe Effects of Algan Hemostatic Agent on bleeding time in a rat tail hemorrhage model(TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2020) Totuk, Ozgan Melike Gedar; Guzel, Sevket Ergun; Ekici, Husamettin; Kumandas, Ali; Aydingoz, Selda Emre; Yilmaz, Enis Cagatay; Kirdan, TaylanBACKGROUND: Algan Hemostatic Agent (AHA) is a multi-herbal extract containing a standardized amount of Achillea millefolium, Juglans regia, Lycopodium clavatum, Rubus caesius or Rubis fruciosus, Viscum album, and Vitis vinifera, each of which is effective in hemostasis. In this study, we aimed to investigate the effects of AHA on bleeding time in a rat tail hemorrhage model. METHODS: Forty-eight Sprague Dawley rats (5-7 weeks old, 180-210 g) were randomly and equally allocated to six groups as follows: heparin plus saline (heparinized control), heparin plus AHA-soaked sponge, heparin plus liquid form of AHA, saline (non-heparinized control), AHA-soaked sponge and liquid form of AHA. Heparin (640 IU/kg) was administered intraperitoneally three times a day for three days in heparinized groups. For the bleeding model, the tail of rats was transected. According to the study group, either saline- or AHA-soaked sponge or liquid form of AHA was applied over the hemorrhage area. In AHA- or saline-soaked sponge groups, once the bleeding time had started, it was checked every 10 seconds. If the bleeding did not stop after 40 seconds, it was accepted as a failure. In liquid AHA group, the duration of bleeding was measured using a chronometer and defined as the time (seconds) from wounding until the bleeding stopped. RESULTS: Bleeding time in the heparinized and non-heparinized control groups was over 40 seconds. After applying the sponge form of AHA on the wound area, bleeding time was significantly shortened to less than 20 seconds in both heparinized and non-heparinized rats (p<0.001 for both). The liquid form of AHA stopped bleeding in 5.0 +/- 1.2 seconds and 8.0 +/- 1.3 seconds in heparinized and non-heparinized groups, respectively. CONCLUSION: AHA is a highly effective topical hemostatic agent in a rat tail hemorrhage model, thus may provide for a unique clinically effective option for control of bleeding during surgical operations or other emergencies.
