Yazar "Ekici, Hüsamettin" seçeneğine göre listele
Listeleniyor 1 - 20 / 26
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Age-and sex-related changes in selected hematological parameters, lipid peroxidation and erythrocytes osmotic fragility of Turkish Angora cats(Hellenic Veterinary Medical Soc, 2022) Kabakcı, Ruhi; Yigit, A. A.; Kara, E.; Çınar, M.; Ekici, Hüsamettin; Aluç, YaşarThis study was conducted to investigate the changes in selected hematological parameters, lipid peroxidation and osmotic fragility of erythrocytes in Angora cats depending on age and gender. For this purpose, the blood samples were collected from vena saphena medialis of 9 young and 14 adult cats which were also classified as male (n=12) and female (n=11). Following hematological analysis, samples were washed with PBS by centrifugation and 10% hematocrit suspension was prepared from the erythrocytes pellet for the osmotic fragility test. The concentration of malondialdehyde (MDA) was also measured from lysed erythrocytes to determine lipid peroxidation level. Red blood cells (RBCs), hemoglobin, and hematocrit were significantly (P<0.001) high in adults while the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly (P<0.05) high in young cats. Erythrocyte MDA level was also higher statistically (P<0.05) in adult cats than in young cats. There was no significance (P>0.05) in these parameters between male and female cats. Findings of fragility tests showed that erythrocytes of young and male cats were statistically more susceptible to hypotonic NaCl solutions than those of adult and female cats, respectively (P<0.01, P<0.05). It was concluded that erythrocytes related parameters in Angora cats changed depending on age rather than gender except for stability of RBCs.Öğe An Investigation of Heavy Metal Concentrations in the Sera of Cattle Grazed in Different Locations in the Kars Province of Türkiye(Springer, 2024) Tarhane, Ayşe Kanıcı; Aluç, Yaşar; Kızıltepe, Semistan; Ekici, HüsamettinThe aim of this study was to investigate the effects of exposure of cattle to low levels of environmental pollution on trace metal metabolism. The assessment of heavy metal concentrations in blood samples is essential to evaluate the potential effects of pollutants on cattle production and to measure the uptake of pollutants by animals. For this purpose, cattle raised in villages at varying distances to the center of the Kars province were sampled for blood, and heavy metal concentrations were measured in the extracted sera. In total 150 blood samples were collected from 4-to 5-year-old cattle from 15 regions. Sera were extracted from the blood samples from the selected foci and analyzed with an inductively coupled plasma-optical emission spectrometry (ICP-OES) device for essential (Co, Cr, Cu, Fe, Mn, Ni and Zn, Se, V) and non-essential (Pb, Cd and Hg, As, Al, Sn) heavy metals. Heavy metals were detected in the serum samples in the following order: Sn > Fe > Al > Zn > Cu > Pb > Cr > As > V > Ni > Mn > Hg. A confidence interval of 95% was used to evaluate all tests. Differences between the villages were insignificant for the Cu, Pb, Cd, Hg, Se, Al, Co concentrations (p > 0.05), whereas differences between the villages were significant for the Fe, As, Ni, Mn (p < 0.05), Zn, Cr (p < 0.01), V and Sn (p < 0.001) concentrations. The results of this study show that heavy metals detected in bovine sera from the sampling area do not exceed the maximum permissible limits, suggesting limited exposure to heavy metals and no associated health risk to animals in the region.Öğe Ankara, Konya ve Bolu illerinden toplanan ruminant ve kanatlı yemlerinde toplam aflatoksin, aflatoksin B1 ve okratoksin A kalıntılarının araştırılması(2011) Altıntaş, Levent; Ekici, Hüsamettin; Yarsan, Ender; Çakır, Serkan; Evrensel, Mehmet Fatih; Tokgöz, Berat SelimBu araştırmada Ankara, Konya ve Bolu illerinden toplanan ruminant ve kanatlı yemlerinde toplam aflatoksin (AfTotal), aflatoksin B1 (AfB1) ve okratoksin A (OA) düzeylerinin ELISA yöntemi kullanılarak belirlenmesi amaçlandı. ELISA yöntemi kullanılarak toplam 150 adet hayvan yemi örneğinde AfB1, AfTotal ve 56 adet örnekte de OA analizleri yapıldı. Numunelerin %90,2’sinde (138 adet) AfB1, %90,2’sinde (138 adet) AfTotal ve %91,07’sinde (51 adet) OA farklı düzeylerde saptandı. Analiz edilen yemlerin %5,07’sindeki AfB1 düzeylerinin (7 adet yem) yemlerde bulunmasına izin verilen değerlerin üzerinde olduğu tespit edildi. Benzer şekilde yemlerin %3,92’sindeki (2 adet) OA değerlerinin yemlerde bulunmasına izin verilen değerlerin üzerinde olduğu bulundu. Sonuç olarak, analizleri yapılan yemlerin büyük bir bölümünün mikotoksinler yönünden bulaşık olmasına rağmen, saptanan bu miktarların örneklerin büyük bir bölümünde yemlerde bulunmasına izin verilen sınırlar içerisinde olduğu gözlendi.Öğe Bolu İlinde Bulunan Ticari ve Damızlık Broiler İşletmelerinde Antibakteriyel İlaçlar için Farmakovijilans Taraması(2015) Ekici, Hüsamettin; Dakman, Asiye; Ekici, Seda; Coşar, Mustafa; Güleç, MetinBu çalışmada hazırlanan farmakovijilans anketinde bakteriyel hastalıkların ticari broiler ve damızlık broiler işletmelerinde görülme sıklığına, antibakteriyel ilaç kullanımından önce antibiyogram yaptırma ve bunların tedaviye yanıtverme durumu ile farmakovijilans bildirimi gibi bazı sorular yöneltilmiştir. Bu amaçla oluşturulan formlar Bolu ilinde ticaribroiler kanatlı sektöründe çalışan 110 adet veteriner hekim (10 adet ticari işletmede) ve 14 adet damızlık broiler sektöründe çalışan veteriner hekimlere (4 adet damızlık işletmede) doğrudan ulaştırılmıştır. Anket çalışması sonucuna göre;işletmelerin son bir yıl içerisinde %92.8'unda (13 adet işletmede) bakteriyel kaynaklı hastalıklar görüldüğü, hastalıkvakalarının %70.16'sında (87/124) antibiyogram yapılmadan antibakteriyel ilaç kullanıldığı, antibiyogram yapılmadanilaç kullanılması durumunda yaklaşık %58.6'sının bazen tedaviye yanıt aldığı, ilaç seçiminde ticari kanatlı sektöründeçalışan veteriner hekimlerin uygulanabilirliğe, damızlık işletmelerde ise edinilebilirliğe önem verdikleri saptanmıştır.Öğe Comparison of the algan hemostatic agent with celox in rat femoral artery bleeding model(2021) Bınnetoglu, Kenan; Kumandaş, Ali; Ekici, Hüsamettin; Özbaykuş, Abdullah Canberk; Tiryaki, MehmetThe most important reasons of deaths after a trauma are major vascular injury. The effective and speedy control of the hemorrhage is crucial in reducing such deaths. Although many products are being used for this topic, most effective products have not yet been developed currently, and there is an urgent need for effective hemostatic. The aim of this study is to compare the efficacy of Algan hemostatic agent (AHA) with Celox in femoral artery incision model in rats. A total of 28 wistar albino rats divided into 4 equal groups. Experimental femoral artery injury was inflicted. In the control group, saline impregnated gauze was employed. AHA impregnated sponge, AHA powder, and Celox in powder form were utilized in the experimental groups. According to the results, there was no difference in bleeding control between AHA and C (p>0.05). When compared to the control group, AHA and Celox were found to be very effective in bleeding control (p<0.001). This study showed that AHA had a similar effect like Celox in controlling bleeding. Considering other characteristics such as AHA's naturalness, easy applicability and low cost, AHA has given hope as an effective hemostatic agent.Öğe Comparison of the Algan Hemostatic Agent with Floseal in Rat Liver Laceration Bleeding Model(Aves, 2022) Binnetoğlu, Kenan; Kumandaş, Ali; Ekici, Hüsamettin; Özbaykuş, Abdullah CanberkObjective: Major vascular injury is one of the most important causes of death after trauma. 'The effective and speedy control of the hemorrhage is crucial in reducing deaths. Many products are used for this purpose. Today, however, an ideal product has not yet been produced and there is a strong demand for such effective hemostatic products. The aim of this study is to compare the efficacy of Algan hemostatic agent with Floseal in the liver laceration model in rats. Materials and Methods: A total of 28 rats were used in the study. Rats were divided into 4 groups, each consisting of 7 rats. Experimental liver laceration was established. In the control group. saline-impregnated gauze was applied. Algan hemostatic agent-impregnated sponge, Algan hemostatic agent powder, and Floseal gel were applied to the experimental groups. Results: There was no difference in bleeding control among the Algan hemostatic agent powder. Algan hemostatic agent-impregnated sponge, and Floseal. When compared to the control group, Algan hemostatic agent powder, Algan hemostatic agent-impregnated sponge, and Floseal were found to be very effective in bleeding control, respectively (P = .001, .012, and .002), in the experimental groups. Conclusion: This study showed that the properties of both Algan hemostatic agent powder and Algan hemostatic agent-impregnated sponge for controlling bleeding are similar to Floseal. Considering other characteristics such as Algan hemostatic agent's naturalness, easy applicability, and low cost, Algan hemostatic agent has been a promising effective hemostatic agent.Öğe Concentration of essential and non-essential elements and carcinogenic / non-carcinogenic health risk assessment of commercial bee pollens from Turkey(Elsevier GmbH, 2023) Sevin, Sedat; Tutun, Hidayet; Yipel, Mustafa; Aluç, Yaşar; Ekici, HüsamettinBackground: Bee pollen, known as a natural super-food with valuable nutritional ingredients, is regarded as a good indicator of ecotoxic substances, such as potentially toxic elements (PTEs). Therefore, this study aims to examine the concentrations of selected PTEs (Al, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Mo, Ni, Pb, Se, Sn, Sr, V, Zn) in bee pollen purchased from online markets in Turkey and perform a health risk assessment to identify the potential risk to consumers. Methods: The quantitative analyses were conducted by inductively coupled plasma optical emission spectrometry (ICP-OES). Results: The mean values of essential PTEs in decreasing content order were Mg > Fe > Zn > Mn > Cu > Ni > Se > Cr > Mo >Co = V. Regarding the results of the study, daily consumption (40 g for adult or 20 g for children) of commercial bee pollen can recompense 20–35 % of daily Cu, Mn, Se requirements for children, adults, pregnant, and breastfeeding women. The decreasing content order of non-essential elements was Al > Sn > Sr > Ba > Pb > As. Cadmium and Hg concentrations were below the detection limits in all the samples. In terms of food and public health; detection of the PTEs concentrations is necessary to assess the quality and safety of bee pollen before consumption. According to the carcinogenic and non-carcinogenic risk assessments; commercial pollen consumption does not pose a health risk to either children or adults for the PTEs monitored in this study. Conclusion: We conclude that bee pollen is an ideal indicator for the monitoring of environmental pollution of PTEs and also a valuable source of essential elements. This study highlights the need to develop standards that regulate acceptable concentrations of PTEs. © 2022 Elsevier GmbHÖğe Concentrations of Essential and Non-essential Toxic Trace Elements in Wild Boar (Sus Scrofa L., 1758) Tissues from Southern Turkey(Springer, 2014) Yarsan, Ender; Yipel, Mustafa; Dikmen, Bilal; Altintas, Levent; Ekici, Hüsamettin; Köksal, AliWild animals, including wild boars, are suitable for use as bioindicators. The aim of this study was to evaluate the concentrations of selected essential (Co, Cu, Fe, Se, Zn) and non-essential (As, Cd, Pb) potentially toxic trace elements in various tissues (hair and hoof) of wild boars hunted in the vicinity of Antalya province in Turkey, in relation to hunting seasons. Concentrations in mg/kg on dry weight basis were determined as 0.37 +/- A 0.27 mg/kg for As, 0.05 +/- A 0.04 mg/kg for Cd, 0.24 +/- A 0.33 mg/kg for Co, 4.84 +/- A 2.48 mg/kg for Cu, 289.94 +/- A 165.26 mg/kg for Fe, 8.71 +/- A 15.68 mg/kg for Pb, 0.24 +/- A 0.10 mg/kg for Se, and 28.99 +/- A 21.41 mg/kg for Zn in the hair samples; and as 0.11 +/- A 0.04 mg/kg for As, 0.01 +/- A 0.04 mg/kg for Cd, 0.01 +/- A 0.01 mg/kg for Co, 1.03 +/- A 0.25 mg/kg for Cu, 56.88 +/- A 18.68 mg/kg for Fe, 0.30 +/- A 0.18 mg/kg for Pb, 0.11 +/- A 0.05 mg/kg for Se, and 17.91 +/- A 10.98 mg/kg for Zn in the hoof samples.Öğe Contractile effects of Eryngium kotschyi Boiss. on rat isolated ileum and detrussor muscle(2014) Baydan, Emine; Kartal, Murat; Dikmen, Begüm Yurdakök; Erdem, Sinem Aslan; İnce, Sinan; Ekici, Hüsamettin; Alp, HarunBu çalışmada, ülkemizdeki endemik bitkilerden Eryngium kotschyi Boiss.in toprak altı (EKTA) ve toprak üstü (EKTU) kısımlarının izole sıçan ileum ve idrar kesesi kasında farmakolojik etkinliği araştırıldı. Bitki ekstrelerinin dokulardaki etkinliği tek, agonist (asetilkolin) ve antagonist (atropin, verapamil, oksibutinin-idrar kesesi, papaverin-ileum) varlığında ve kalsiyumsuz ortamda Ca 2 uygulamaları ile birlikte değerlendirildi. Bitkinin her iki kısmı doku türü, ekstre dozu ve uygulama protokolüne bağlı değişiklik gösterecek şekilde kontraksiyon oluştururken; bu kasılmaların EKTU ve EKTA tek uygulamalarında doza bağımlı, kümülatif uygulamalarında ise dozdan bağımsız olduğu görüldü. Oluşan kasılmaların test edilen antagonistler ile değiştiği; dolayısıyla kontraktil etkinliğin kalsiyum iyonu ve kalsiyum kanallarının uyarılması gibi nonspesifik yolaklara özellikle bağlı olabileceği görüşüne varıldı.Öğe Determination of blood heavy metal concentrations and oxidant-antioxidant capacities in Angora cats at different age and gender(Ankara Univ, 2023) Kabakçı, Ruhi; Yiğit, Ayşe Arzu; Ekici, Hüsamettin; Aluç, Yaşar; Kara, ErdalThis study was conducted to investigate heavy metal concentrations and oxidative status of plasma and erythrocytes in Angora cats at different ages and gender. Sixteen young (less than 1 year old) and 14 adult (1-6 years old) cats were also grouped according to gender as male (n = 17) and female (n = 13). The separated plasma samples from cat's blood were analyzed for selected heavy metals and total oxidant and antioxidant capacities (TOC and TAC) and calculated for oxidative stress index (OSI). The erythrocyte hemolysates were also evaluated for malondialdehyde (MDA), and super oxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX). Results of the study showed that most of the measured metals were not varied statistically according to age or gender. However, adult cats had significantly (P<0.01) higher Cu and lower Fe levels compared to young cats. Plasma levels of TOC, TAC and OSI, and erythrocyte MDA concentrations in young cats were significantly (P<0.05) higher than that of adults. While the SOD activity was decreased by the age, GPX activity was increased (P<0.05). However, the activity of CAT was changed by only gender, which was higher in males (P<0.01). In conclusion, metals, especially trace elements, are required for many kinds of physiological processes and the synthesis of antioxidant enzymes. Therefore, it can be suggested that the periodic measurement of metals and the addition of common antioxidant supplements to the diet of adult Angora cats will support weakening antioxidant mechanisms by age.Öğe The effect of seasonal variations on the occurrence of certain mycotoxins inconcentrate feeds for cattle collected from some provinces in Turkey(2016) Ekici, Hüsamettin; Yıldırım, Ebru; Yarsan, EnderThe aim of this study was to determine the effects of seasonal variations on the occurrence of total aflatoxin (AFTotal), aflatoxin B1 (AFB1), ochratoxin A (OTA), and total fumonisin (FTotal) in the commercially mixed ruminant feed obtained from feed mills located in Ankara, Kırıkkale, çankırı, çorum, and Kırşehir provinces using the enzyme-linked immunoassay (ELISA) method. To represent the annual production, 22 samples were collected every quarter over 1 year (a total of 88 samples) starting in September 2012. AFTotal and AFB1 were detected in 72 samples (81.81%), OTA in 84 samples (95.45%), and FTotal in 83 samples (94.31%). When seasonal changes were taken into consideration, the highest levels of AFTotal, AFB1, and FTotal (13.57 ± 8.78 ppb, 8.54 ± 6.02 ppb, and 0.70 ± 0.88 ppm, respectively) were found in the summer, while the highest OTA level (57.69 ± 14.59 ppb) was observed in the spring. The results of high-performance liquid chromatography methods confirmed the results of the ELISA method. Consequently, although most of the feed samples were contaminated with mycotoxins, all the amounts were within the limits allowed for feedstuffs in Turkey.Öğe Effectiveness of Algan Hemostatic Agent in Bleeding Control: An Experimental Kidney Incision Model(Galenos Yayincilik, 2021) Midi, Ahmet; Kumandaş, Ali; Ekici, Hüsamettin; Kılıç, Yusuf; Özgöçmen, RuchanAim: The purpose of this study is to evaluate the hemostatic efficacy of a novel plant originated blood stopper which is called Algan Hemostatic Agent (AHA) in uncontrolled kidney incision bleeding model. Methods: The study was conducted with thirty-two rats 5-7 weeks old. The rats were randomly divided into 4 groups, each consisting of eight rats (Group 1: heparinized saline, 2: heparinized AHA, 3: non-heparinized saline, 4: non-heparinized AHA). Experimental kidney incision was made, and saline soaked sponge was applied to the control group and AHA to the study group. Results: The duration of bleeding was significantly shorter in the AHA group compared to the control group. The AHA has controlled the bleeding in the heparinized and non-heparinized groups 5 +/- 1.19 and 3 +/- 0.91 seconds respectively (p<0.001). In the control group, hemorrhage was controlled in the heparinized and non-heparinized group 180.6 +/- 25.4 and 125.4 +/- 21 seconds respectively. Conclusion: This study demonstrated that the AHA is useful in controlling bleeding in the Rat kidney incision model.Öğe Investigation of the biocompatibility and in vivo wound healing effect of Cotinus coggygria extracts(Ankara Univ, 2024) Bozkaya, Esra; Türk, Mustafa; Ekici, Hüsamettin; Karahan, SiyamiCotinus coggygria is widely recognized its antiseptic, anti-inflammatory, antimicrobial, antihemorrhagic, and wound-healing properties. In this, aimed to evaluate the phenolic contents, cytotoxicity/proliferation, hemolytic, antimicrobial, genotoxic, apoptotic, necrotic activities, and in vivo wound healing effects of C. coggygria, , a plant species known to have beneficial effects on wound healing. TOF-LC/MS analyzes revealed that the methanol extract of C. coggygria leaves contained flavonoids and phenolic compounds such as gallic acid (18.5 mg/kg), catechin (4.6 mg/kg), protocatesic acid (0.6 mg/kg), vanillic acid (8.4 mg/kg), ellagic acid (0.1 mg/kg), rosmarinic acid (0.1 mg/kg), quercetin (15 ppb) and C. coggygria stems contained such as gallic acid (24.6 mg/kg), catechin (155.1 mg/kg), chlorogenic acid (1.9 mg/kg), 4hydroxybenzoic acid (383.3 mg/kg), rutin (2.5 mg/kg), ellagic acid (15.1 mg/kg), apigenin 7-glycoside (10.5 mg/kg), rosmarinic acid (0,4 mg/kg), quercetin (15.2 mg/kg), naringenin (279.1 mg/kg). Consequently, C. coggyria has a positive effect on wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, or hemolytic effects at test concentrations. In the in vivo burn model, wounds treated with leaf and stem extracts healed faster than the control group. Thus, C. coggygria is an effective plant for wound healing with antibacterial properties, particularly against E. coli, and without cytotoxic, genotoxic, and hemolytic effects.Öğe Investigation of The Effectiveness of Plant Based Algan Hemostatic Agent in a Rat Model of Femoral Arterial Bleeding(Bezmialem Vakif Univ, 2022) Ekici, Hüsamettin; Kumandaş, Ali; Özbaykuş, Abdullah Canberk; Dizdaroglu, Hazal; Midi, Ahmet; Balik, Mehmet Sabri; Yesilada, ErdemObjective: The aim of this study is to evaluate the efficacy of the Algan Hemostatic Agent (AHA) available in three different physical form (liquid, powder and sponge absorbed) in the femoral artery incision model in rats. Methods: A total of sixty-four 5-7 weeks old rats were used in the study. Rats were randomly divided into 8 groups each consisting of eight rats (4 groups heparinized and 4 groups non-heparinized). An experimental femoral artery incision was created. As a control, physiological saline absorbed sponge was applied. AHA liquid, AHA powder and AHA sponge absorbed forms were applied to the experimental groups. Results: Upon application to the bleeding sites, all the AHA forms stopped bleeding in a significantly shorter time compared to the control group (p<0.05). In contrast, bleeding in control group could not be controlled within 4 minutes. The best result was in AHA powder form and it was able to control bleeding in the non-heparinized group at 87.5% in the first minute and 12.5% in the second minute. In the heparinize group, the AHA powder form was able to control the bleeding at 62.5% in the first minute and 37.5% in the second minute. Conclusion: This study shows that AHA is a highly effective and promising hemostatic agent in bleeding control.Öğe Investigation of the Efficacy of Algan Hemostatic Agent in Liver Laceration Model in Rats(2019) Midi, Ahmet; Kumandaş, Ali; Ekici, Hüsamettin; Bayraktar, Feyzanur; Karapirli, Kübra; Karahan, Siyami; Özyürek, H. EmelObjectives: Bleeding control is crucial in preventing negative consequences by reducing blood loss in surgical operations. The aim of this study is to evaluate the hemostatic effect of a new herbal hemostatic agent called Algan Hemostatic Agent (AHA) in an uncontrolled bleeding model made by liver laceration.Methods: In these study 5–7 weeks-old 64 rats were used. Rats were randomly divided into 8 groups each consistingof eight rats (4 groups heparinize and 4 groups non-heparinize). The experimental liver laceration was performed, andphysiological serum impregnated gauze was applied to the control group for hemorrhage control, AHA liquid formimpregnated gauze, AHA gel, and AHA powder form were applied to experimental groups, respectively.Results: The shortest bleeding time was found in the AHA powder group. The AHA powder form stopped the bleedingin the heparinize group for a mean of 4 s, the non-heparinized group for 2 s. This was followed by the gel group and theliquid group. The bleeding time was significantly shorter in the all AHA group compared to the control group.Conclusion: This study showed that AHA is a highly effective hemostatic agent in controlling bleeding compared tothe control group.Öğe Investigation of the in vitro antibacterial, cytotoxic and in vivo analgesic effects of silver nanoparticles coated with Centella asiatica plant extract(Ankara Univ, 2023) Bozkaya, Ogun; Ekici, Hüsamettin; Gün Gök, Zehra; Arat, Esra; Ekici, Seda; Yigitoglu, Mustafa; Vargel, İbrahimIn recent years, researchers have shown an increased interest in using medicinal plants for the synthesis of silver nanoparticles (AgNPs) having various therapeutic properties. Centella asiatica (CA), a medicinal plant, has been used to treat minor burn wounds, psoriasis, and hypertrophic wounds among many other pathological conditions. The current study aimed to synthesize CA coated AgNPs (CA-AgNPs) with appropriate biocompatibility and various therapeutic properties, including antimicrobial and analgesic activities. The synthesized CA-AgNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, zeta potential measurements, and fourier transform infrared (FT-IR) spectroscopy. The formation of spherical CA-AgNPs was confirmed by a single surface plasmon resonance (SPR) peak emerging at 420 nm wavelength by UV-Vis. The average hydrodynamic diameter and zeta potential of the particles were found to be 29.5 nm and -24.5 mV, respectively. The FT-IR analyses showed that the AgNPs were coated and stabilized by bioactive compounds from the CA extract. MTT cytotoxicity assay revealed that CA-AgNPs at <= 1 mM concentrations exhibited biocompatibility for L929 fibroblast cells. The antimicrobial activity of CA-AgNPs was confirmed by significant inhibition of Staphylococcus aureus and Escherichia coli. In addition, the analgesic effect of CA-AgNPs was investigated for the first time in the literature by tail-flick and hot plate methods, and statistically significant results were obtained for both methods. Taken together, these results suggest that CA-AgNPs can be used as an effective antibacterial and analgesic agent in a variety of biomedical applications, including coating wound dressings.Öğe Karadeniz, Marmara, Ege ve Akdeniz'de Avlanan Kabuklularda Organokalay (Butilkalay ve Fenilkalay) Bileşiklerinin Araştırılması(2024) Ekici, Hüsamettin; Yipel, MustafaGünümüzde çevre koşulları göz önüne alındığında sürdürülebilir çevre açısından kirleticilerin varlıkları, düzeyleri ve etkilerinin izlenerek gerekli önlemlerin alınması önemli bir kavram olarak karşımıza çıkmaktadır. Organometalik kimya ve katalizin son yıllardaki hızlı gelişimi, modern kimya biliminin yönünü değiştirmiş ve organik sentez, farmasötik uygulamalar, kimya endüstrisi, malzeme bilimi, enerji araştırmaları ve diğer birçok alana çok sayıda katkı sağlamıştır. Çevrede fazla miktarda organometalik bileşik bulunmaktadır. Çevre, okyanuslar, içme suyu kaynakları, okyanuslarda tortullar, bitki örtüsü ve hayvan yaşamı ile ilişkilidir. Bu bileşikler insan ve hayvan sağlığı açısından risk oluşturmaktadır. Bu nedenle meydana gelen organometalik bileşiklerin detaylı olarak bilinmesi gerekmektedir. Organokalay kirletici olan tribütilkalay (TBT) ve Tetrabütilkalay (TetraBT), çevresel endokrin bozucu bir kimyasaldır ve bilinen bir obezojen ve diyabetojendir. Tetrabütilkalay, tribütilkalay ve dibütilkalay bileşiklerinin öncüsüdür. Çevresel endokrin bozucu kimyasallar, endokrin sisteme müdahale ederek zararlı etkilere neden olabilmekte, insan ve yaban hayatı popülasyonlarını olumsuz yönde etkileyebilmektedir. Çalışmada su ürünleri kooperatif ve satış noktalarından yasal mevzuat doğrultusunda Karadeniz, Marmara, Ege ve Akdeniz?de avlanmış beyaz kum midyesi (Chamelea gallina), pembe karides (Parapenaeus longirostris) ve deniz salyangozu (Rapana thomasiana) örneklerinde debutilkalay (dibütilkalay [DBT], tribütilkalay [TBT] Tetrabütilkalay [TetraBT]) ve fenilkalay (monofenilkalay [PhT], difenilkalay [DPhT], trifenilkalay [TPhT]) kontaminasyon düzeylerinin GC-MS ile analiz edildi. Yapılan analiz sonucunda örneklem yapılan bölgelerden alınan numunelerde butilkalay [dibütilkalay (DBT), tetrabütilkalay (TetraBT)] ve fenilkalay [monofenilkalay (PhT), difenilkalay (DPhT), trifenilkalay (TPhT)] kontaminasyon düzeyleri tespit limitlerinin (LOD) altında olduğu tespit edildi. Yapılan bu çalışmada kullanılan ekstraksiyon metodu ve GC-MS analizlerinden elde edilen tespit limitleri örneklem yapılan alanlardaki kirlilik düzeylerinin tam tespiti için yeterli olarak görülmemiştir. Dolayısıyla daha farklı yöntemlerin denenmesi önerilmiştir. Ayrıca, bu organokalay bileşiklerinin zehirlilik potansiyeli ve artan kullanımları dolayısıyla organokalaylı bileşiklerin gıda zincirine katılarak insan sağlığı üzerinde oluşturacakları risklerin araştırılacağı çalışmalara da ilginin artacağı da düşünülmektedir.Öğe Protective Effect of Krill Oil Against Gentamicin Induced Oxidative Stress Mediated Nephrotoxicity in Rats(2022) Şahin, Yaşar; Alçığır, Mehmet Eray; Şenol, Ali; Özden, Hüseyin; Ekici, Hüsamettin; Yıldırım, Ebru; Çınar, MiyaseThis study aimed to evaluate the protective effect of krill oil against nephrotoxicity caused by gentamicin. Distilled water was given orally to the control and second groups (GI) for seven days while 500 mg/kg krill oil was given to the third (GII), fourth (GIII) groups. In addition, isotonic saline was administered subcutaneously to the control and GIII groups throughout the study, while 80 mg/kg gentamicin was administered to the GI, and GII groups. Alanine aminotransferase (ALT) and gamma glutamyltransferase (GGT) peptidase, total cholesterol, urea, and creatinine levels in plasma and, malondialdehyde (MDA) and total antioxidant status (TAS) levels in both plasma and kidney tissue supernatant were evaluated. Histopathological changes in tubules and glomeruli and vascular changes were evaluated by scoring. Urea level and ALT activity were found to be significantly lower in the GII and GIII groups compared to the GI group (p<0.001; p?0.001). As a result, it was observed that degenerative damage and glomerular changes in the tubule at the histological level mediated by oxidative stress were consistent with the increase in ALT, urea, and MDA levels. In this respect, it is suggested that krill oil can be used as a nephroprotective food supplement to contribute to treatment in cases of toxicity.Öğe Protective Effect of Silymarin and Boric Acid Against Isoproterenol-Induced Myocardial Infarction in Mice(2022) Şahin, Yaşar; Alçığır, Mehmet Eray; Bakar-Ateş, Filiz; Ekici, Hüsamettin; Şenol, Ali; Atıcı, Enes Gazi; Yıldırım, EbruThis study aimed to compare the protective effects of separate and combined use of silymarin and boric acid against myocardial infarction induced by isoproterenol. Distilled water was given orally by gavage to the GI and GII groups for fourteen days. The GIII group was received silymarin (100 mg/kg), while the GIV group was given boric acid (100 mg/kg) for 14 days. In the last group, both silymarin and boric acid were given orally (gavage) for fourteen days. On the thirteenth and fourteenth days of the study, while isotonic water was applied to the GI group, isoproterenol (85 mg/kg) was applied to the other groups subcutaneously. Plasma ALT, AST, Ca, CK, CHO, TP, Ing P, IL-6, IL-12, PAL-1, and sPLA2 parameters were evaluated. Histopathological examination was performed on the heart, lung, liver, kidney, spleen, cerebrum, and cerebellum tissues taken from mice. The CK level was found to be significantly lower in the GIII and GV groups compared to the GII group (P<0.05). As a result, the increases shown in the plasma AST and CK activities caused due to the myocardial damage induced by isoproterenol were decreased in the silymarin and boric acid applied groups. Therefore it was thought that silymarin and boric acid may contribute to protection against myocardial infarction.
