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Öğe Antibacterial and antifungal activity of Heracleum sphondylium subsp artvinense (conferenceObject)(Elsevier Science Bv, 2005) Kaçar, Y.; Tan, S.; Ergene, A.; Güler, P.; Mirici, S.; Hamzaoglu, E.; Yildirim, S.…Öğe Biosorption of copper(II) on immobilised Synechocystis aquatilis(Parlar Scientific Publications (P S P), 2006) Ergene, A.; Tan, S.; Katircioglu, H.; Öktem, Z.Synechocystis aquatilis was immobilized in Ca-alginate via entrapment. The plain alginate, and alive and heat-inactivated immobilized algae beads were used for the sorption of Cu2+ ions from aqueous solutions at 25 degrees C. Kinetic studies showed a fast initial rate, followed by a slower biosorption one. Adsorption of Cu2+ on the biosorbents increased with increasing initial Cu2+ ions level in the solution. The heat-inactivated alga preparation showed a higher biosorption capacity than the alive counterpart. The biosorption equilibrium was established approx. in 3 h, and the equilibrium was well-described by the Langmuir and Freundlich adsorption isotherms. The effect of pH was also investigated and the maximum adsorption of Cu2+ ions on the biosorbents was obtained approx. at pH 5.6.Öğe Ca-alginate as a support for Pb(II) and Zn(II) biosorption with immobilized Phanerochaete chrysosporium(Elsevier Sci Ltd, 2003) Arica, M.Y.; Arpa, C.; Ergene, A.; Bayramoglu, G.; Genç, O.The basidio spores of Phanerochaete chryosporium were immobilized in alginate gel beads, and the immobilized spore containing alginate beads were incubated for the growth of fungus. The biosorption of Pb2+ and Zn2+ ions on alginate beads and both immobilized live and heat inactivated fungus was studied from artificial waste waters in the concentrations range of 30-600 mg 1(-1). The surface charge density of the biosorbents varied with the pH of the medium and the maximum biosorption of heavy metal ions on the biosorbents was obtained between pH 5.0 and 6.0. The biosorption of Pb2+ and Zn2+ on the biosorbents increased as the initial concentration of Pb2+ and Zn2+ ions increased in the medium. Biosorption equilibrium was established about 1 h, the adsorbed heavy metal ions did not significantly change further with time. The maximum biosorption capacity (q(m)) of alginate beads and both immobilized live and heat inactivated fungus were 230, 282 and 355 mg for Pb2+ and 30, 37 and 48 mg for Zn per gram of dry biosorbents, respectively. The experimental biosorption equilibrium data for Pb2+, and Zn2+ ions were in good agreement with those calculated by Langmuir model. The affinity order of heavy metal ions was Pb2+ > Zn2+, (C) 2003 Elsevier Science Ltd. All rights reserved.Öğe Nigella sativa seed-mediated green biosynthesis of silver nanoparticles and antimicrobial activity(Soc Mexicana Fisica, 2024) Boke, E.; Saygi, K. Ozlem; Erenler, R.; Kacmaz, B.; Ergene, A.This work introduces a systematic and efficient approach for producing stable AgNPs utilizngNigella sativa (Ns) seed aqua extract (AE), which exhibit strong antibacterial properties. The characterization of Ns-AgNPs was performed using a UV-visible spectrophotometer electronic microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). The Ns-AgNPs did not show aggregation, as shown by the results of STEM and XRD. According to the EDX analysis in this research, it was determined that Ns-AgNPs, gave signals in the silver region (similar to 3 KeV) at 92.3%. Dynamic light scattering (DLS) was used to determine the average particle size and distribution profile of NsAgNPs. Ns-AgNPs showed significant antibacterial performance againstStaphylococcus aureousand Escherichia coli, being effective at low concentrations. Ns-AgNPs may be incorporated into wound dressings, surgical instruments, and medical devices to prevent infections and promote healing.Öğe Reversible immobilization of lipase on phenylalanine containing hydrogel membranes(Elsevier Sci Ltd, 2001) Arıca, M.Y.; Kaçar, Y.; Ergene, A.; Denizli, A.Poly(2-hydroxyethylmethacrylate-co-methacrylamidophenlyalanine) poly(HEMA-MAPA) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA. Lipase immobilization onto these membranes from aqueous solutions containing different amounts of lipase at different pH was investigated in a batch system. The lipase adsorption capacity of the membranes was increased as the MAPA ratio increased in the membrane structure. The maximum lipase immobilization capacity of the poly(HEMA-MAPA-3) membrane was 135 mug cm(-2). The optimum temperature was 5 degreesC higher than that of the free enzyme and was significantly broader. The storage stability increased with immobilization. The enzyme could be repeatedly adsorbed and desorbed without any significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.