Reversible immobilization of lipase on phenylalanine containing hydrogel membranes
Yükleniyor...
Tarih
2001
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Poly(2-hydroxyethylmethacrylate-co-methacrylamidophenlyalanine) poly(HEMA-MAPA) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA. Lipase immobilization onto these membranes from aqueous solutions containing different amounts of lipase at different pH was investigated in a batch system. The lipase adsorption capacity of the membranes was increased as the MAPA ratio increased in the membrane structure. The maximum lipase immobilization capacity of the poly(HEMA-MAPA-3) membrane was 135 mug cm(-2). The optimum temperature was 5 degreesC higher than that of the free enzyme and was significantly broader. The storage stability increased with immobilization. The enzyme could be repeatedly adsorbed and desorbed without any significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.
Açıklama
Kacar, Yasemin/0000-0002-8682-9228
Anahtar Kelimeler
adsorption, immobilization, lipase, phenylalanine, poly(HEMA) membrane
Kaynak
Process Biochemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
36
Sayı
8-9
Künye
closedAccess
