Reversible immobilization of lipase on phenylalanine containing hydrogel membranes

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dc.contributor.authorArıca, M.Y.
dc.contributor.authorKaçar, Y.
dc.contributor.authorErgene, A.
dc.contributor.authorDenizli, A.
dc.date.accessioned2020-06-25T17:34:52Z
dc.date.available2020-06-25T17:34:52Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.descriptionKacar, Yasemin/0000-0002-8682-9228
dc.description.abstractPoly(2-hydroxyethylmethacrylate-co-methacrylamidophenlyalanine) poly(HEMA-MAPA) membranes were prepared by UV-initiated photopolymerization of HEMA and MAPA. Lipase immobilization onto these membranes from aqueous solutions containing different amounts of lipase at different pH was investigated in a batch system. The lipase adsorption capacity of the membranes was increased as the MAPA ratio increased in the membrane structure. The maximum lipase immobilization capacity of the poly(HEMA-MAPA-3) membrane was 135 mug cm(-2). The optimum temperature was 5 degreesC higher than that of the free enzyme and was significantly broader. The storage stability increased with immobilization. The enzyme could be repeatedly adsorbed and desorbed without any significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0032-9592(00)00289-2
dc.identifier.endpage854en_US
dc.identifier.issn1359-5113
dc.identifier.issue8-9en_US
dc.identifier.scopus2-s2.0-0035073382
dc.identifier.scopusqualityQ1
dc.identifier.startpage847en_US
dc.identifier.urihttps://doi.org/10.1016/S0032-9592(00)00289-2
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2936
dc.identifier.volume36en_US
dc.identifier.wosWOS:000168163200020
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofProcess Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectadsorptionen_US
dc.subjectimmobilizationen_US
dc.subjectlipaseen_US
dc.subjectphenylalanineen_US
dc.subjectpoly(HEMA) membraneen_US
dc.titleReversible immobilization of lipase on phenylalanine containing hydrogel membranesen_US
dc.typeArticle

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