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    Crossroad between the sylvatic and domestic cycles of Mesocestoides litteratus: mesocestodiasis in dogs adopted from shelters in Türkiye
    (Springer, 2024) Akdeniz, Sinem; Akkus, Gozde Nur; Avci, Beyza; Gazyagci, Aycan N.; Yildiz, Kader
    This study aimed to report the presence of Mesocestoides litteratus in dogs adopted from shelters in T & uuml;rkiye. Gravid segments were examined microscopically in the faeces of dogs from different shelters located in Ankara and Kirikkale provinces in the central region of T & uuml;rkiye. Then, genomic DNA obtained from these segments, a 446-bp fragment of the mitochondrial cytochrome C oxidase subunit 1 gene, and a 350-bp fragment of mitochondrial 12S rRNA were amplified and sequenced. BLASTn search was performed. During light microscopic examination, an egg-filled paruterine organ was observed in the middle part of the segment. Thin-shelled, oval, 35-mu m-diameter parasite eggs containing an oncosphere with three pairs of hooklets were observed. The gravid segments were determined as Mesocestoides spp. based on the appearance of the typical paruterine organ. PCR results supported our diagnosis; moreover, according to the BLAST results, it was detected that the species infecting two dogs was 98.01-100% similar to M. litteratus. Praziquantel-containing medication was administered to the infected dogs at a dosage of 5 mg/kg. Foxes act as the final host of M. litteratus and the parasite is prevalent in wildlife; however, these animals may disperse the parasite in urban life. Veterinarians need to be made more aware of this parasite, especially if the dogs are owned from shelters.
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    Detection and isolation of Leishmania infantum from natural infected dog in Türkiye
    (Elsevier, 2025) Damlapinar, Aysegul; Berberoglu, Umut; Mungan, Mesut; Celebi, Bekir; Gazyagci, Aycan N.; Yildiz, Kader
    Some clinical signs such as cachexia, alopecia, exfoliative dermatitis, hair loss, and swollen lymph nodes were observed in a one-year-old crossbred male dog living in a rural area of Kirikkale province, T & uuml;rkiye, was presented to the veterinary clinic by its owner. Anaemia, leucopenia, hyperglobulinemia, and bilirubinemia were detected. Seropositivity was detected using Leishmania IgG/IgM Rapid Test. The amastigote forms of the parasite were observed in the lymph node aspirates. Viable promastigotes were observed in the samples obtained from the Novy-MacNeal-Nicolle medium (NNN) tubes. The parasite DNA was extracted from the promastigotes produced in the NNN medium using a DNA extraction kit according to the manufacturer's instructions. The specific bands indicating the gene regions of L. infantum (350 bp and 730 bp for HSP20 and HSP70, respectively) were observed. Additionally, BLASTn analysis revealed 100 % similarity with GenBank-deposited L. infantum sequences. The sequences were submitted to GenBank (Accession numbers: OR806945 and OR806946). According to the author's knowledge, it is the first dog isolate deposited in the collection of the Microbiology Reference Laboratories and Biological Products Department, General Directorate of Public Health, Ministry of Health, Ankara, T & uuml;rkiye. The isolate (TR_L. infantum CanL-1_damla) has been preserved by cryopreservation at -150 degrees C for further studies.
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    The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kirikkale Region
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Yildiran, F. Azize Budak; Yildiz, Kader; Cakir, Suekran; Gazyagci, Aycan N.
    In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCR using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, OPB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain.