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Öğe Astrocytes, microglia/macrophages, and neurons expressing Toll-like receptor 11 contribute to innate immunity against encephalitic Toxoplasma gondii infection(Pergamon-Elsevier Science Ltd, 2014) Atmaca, H. T.; Kul, O.; Karakus, E.; Terzi, O. S.; Canpolat, S.; Anteplioglu, T.Toll-like receptor 11 (TLR11) is a specific receptor for Toxoplasma gondii and uropathogenic Escherichia coli and has recently been identified in the mouse brain. In the present study, TLR11 gene expression was measured in the mouse brain by Real-time quantitative polymerase chain reaction (RT-PCR). Furthermore, the TLR11 protein expression profile was evaluated in neuroglia and neurons throughout the encephalitic period (10, 20, and 30 days after inoculation) in mice with experimentally induced T. gondii infection. In the brains of experimental (n = 21) and control (n = 7) mice, TLR11, glial fibrillary acidic protein (GFAP), cd11b, NeuN, TLR11/GFAP+, TLR11/cd11b+, and TLR11/NeuN+ cells were investigated using either indirect single- or double-labeling immunoperoxidase staining. The results indicated that TLR11 gene expression increased during chronic toxoplasmic encephalitis, and there was a variable degree of TLR11 immunopositivity among cd11b+, GFAP+, and NeuN+ cells in the brain. On the tenth day of infection, there was a significant increase in TLR11 protein and gene expression, which remained stable during the later stages of infection. In this experimental model, TLR11 expression was induced in astrocytes, neurons, and microglia/macrophages during the immune response to T. gondii infection. (C) 2014 IBRO. Published by Elsevier Ltd. All rights reserved.Öğe Concurrent peste des petits ruminants virus and pestivirus infection in stillborn twin lambs(Amer Coll Vet Pathologist, 2008) Kul, O.; Kabakci, N.; Ozkul, A.; Kalender, H.; Atmaca, H. T.Concurrent infection with peste des petits ruminants virus (PPRV) and pestivirus was diagnosed in stillborn twin lambs. With the flock history, the findings of epidermal syncytial cells and necrotizing bronchitis/bronchiolitis prompted testing for PPRV infection, and PPRV antigen was detected by immunohistochemistry (IHC) in the skin, lungs, kidneys, rumen, and thymus. Macroscopic anomalies that were typical of border disease included scoliosis, brachygnathism, prognathism, arthrogryposis, hydranencephaly, cerebellar hypoplasia, and hairy fleece; pestiviral antigen was detected by IHC in the brain, liver, lungs, and kidneys. Tissues from both lambs were positive by reverse transcriptase-polymerase chain reaction (RT-PCR) for PPRV and pestivirus. To the authors' knowledge, PPR has not been reported previously as a congenital infection or in combination with pestiviral infection.Öğe Congenital chondrodysplasic dwarfism in lambs: A diferent consideration of dystocia(Israel Veterinary Medical Assoc, 2010) Yagci, Pir, I; Kul, O.; Kalender, H.; Polat, I. M.In this study, we report three chondrodysplasic anomalous lambs obtained from 2 Akkaraman sheep with dystocia. According to the flock history, the incidence of congenital skeletal anomalies among lambs was 14.28 % and 11.66 % in 2005 and 2006, respectively. The same two rams had been used to obtain natural mating and no new animals had been bought into the flock over the last 5 years. Affected lambs showed typical macroscopic features of a compressed and flattened face, domed head, narrow thorax, swollen abdomen and extremely short paddle like extremities. Radiological examination of extremities of the long bones revealed that diaphyses were extremely shortened and distance between diaphysis and epiphysis was increased. Long bones such as antebrachium, humerus, tibia and femur were short and thick. Histopathologically, immature chondrocytes were present at the articular surface and defective epiphyseal plates were detected in the extremities of the bones and sternum. Persistent cartilage showed irregular strands containing groups of two or four chondrocytes localized in the basophilic matrix. In conclusion, chondrodysplasia of lambs should be added to the list of differential diagnoses of dystocia especially in inbreed sheep.Öğe The effects of ozone therapy on cavernosal damage due to ischemia / reperfusion injury induced priapism in rat models(Wiley-Blackwell, 2011) Basar, M.; Aydos, T.; Kul, O.; Atmaca, T.; Uzunalioglu, T.; Kisa, U.…Öğe Investigation of Microbiological, Physiological and Histopathological Changes in Polymicrobial I nfection in Old Rats(Agricultural Research Communication Centre, 2024) Ulker, U.; Kizil, S.; Bayraktar, B.; Alcigir, M. E.; Kul, O.; Kaya, H.; Urusan, H.Background: The aim of this study is to examine the microbiological, clinical, physiological and pathological findings of natural polymicrobial infection developing in a wistar rat colony under long-term observation. Methods: In this study, 70 male Wistar albino rats, 3 years old, raised by conventional methods and housed in a cage environment were used. Thirteen rats with clinical symptoms such as torticollis and rotation were sacrificed for microbiological and histopathological examination after 14 days. For microbiological examination, the rapid diagnostic kit BBL Crystal system was used to identify bacterial agents in tissue and stool samples and the traditional culture method was used for isolation. Result: As a result of microbiological analysis, the rarely isolated Staphylococcus capitis ( S. capitis ) was isolated. Different Staphylococcus spp. It has been determined that the species can cause suppurative meningoencephalitis as well as septicemia in the brains of experimental animals., it is thought that polymicrobial infection will be useful for the identification of bacterial agents and infection prevention and treatment strategies.Öğe Left ventricular mass in a Simmental calf: cavernous haemangioma(Schattauer Gmbh-Verlag Medizin Naturwissenschaften, 2011) Atmaca, H. T.; Kul, O.; Dincel, G. C.; Terzi, O. S.…Öğe Natural peste des petits ruminants virus infection: Novel pathologic findings resembling other morbillivirus infections(Sage Publications Inc, 2007) Kul, O.; Kabakcı, N.; Atmaca, H. T.; Özkul, A.The present study describes pathologic and virologic findings in 15 sheep and 6 goats that died of natural peste des petits ruminants virus Infection in Turkey. Pathologic findings included erosiveulcerative stomatitis, fibrino-necrotic tracheitis, bronchointerstitial pneumo nia, multifocal coagulation necroses in the liver, and severe lymphocytolysis in lymphoid tissues. Syncytial cells were conspicuous, especially in the oral mucosa, pulmonary alveoli, liver, and lymphoid tissues. In addition to the typical tissue distribution, eosinophilic intracytoplasmic and/or intranuclear inclusions were observed in epithelia cells lining the renal pelvis and abomasal mucosa. Inimunolabeling of the viral antigen was observed in the kidney, brain, rumen, abomasum, heart, and myocytes of the tongue besides its more typical locations. III this study, we report and describe in detail the first peste des petits ruminants endemic in Kirikkale Province, Central Anatolia of Turkey. In conclusion, these previously unreported pathologic findings in natural peste des petits ruminants virus infection establish a basis for resemblance to other morbillivirus infections, such as canine distemper and distemper of sea mammals. Reverse t ran script a se-po lymerase chain reaction analyses indicated that the 448-bp genome fragment was amplified in 18 cases (18/21, 85.7 %). Phylogenetic analysis showed that viruses belong to lineage 4 in the peste des petits ruminants virus common phylogenetic tree.Öğe Neospora caninum: the First Demonstration of the Enteroepithelial Stages in the Intestines of a Naturally Infected Dog(Elsevier Sci Ltd, 2015) Kul, O.; Atmaca, H. T.; Anteplioglu, T.; Ocal, N.; Canpolat, S.A 1.5-month-old Kangal breed puppy from a dairy cattle farm died after showing severe diarrhoea and incoordination. Necropsy examination revealed multifocal pulmonary consolidation and necrosis and fibrinohaemorrhagic enteritis. Microscopically, there was necrotic and purulent bronchopneumonia, myocarditis and non-purulent encephalitis. In the jejunum and ileum there was villous atrophy and crypt hyperplasia with oocyst-like and schizont-like structures in the epithelia. Immunohistochemically, Neospora caninum antigen was detected in association with the intestinal protozoal structures, degenerative neurons and areas of necrosis in the lungs and heart. Polymerase chain reaction confirmed that the organism was N. caninum and not Toxoplasma gondii. The seroprevalence for N. caninum was 74.2% (49/66 animals) for the cattle and 57.1 A (4/7 animals) for dogs on this farm. This report documents fatal systemic neosporosis and enteroepithelial stages of N. caninum in a naturally infected puppy. To the authors' knowledge, this is the first definition of intestinal neosporosis in a naturally infected dog as well as the first evidence of fatal canine neosporosis in Turkey. (C) 2015 Elsevier Ltd. All rights reserved.Öğe Rare gallbladder parasitosis mimicking cholelithiasis: Dicrocoelium dendriticum(Georg Thieme Verlag Kg, 2008) Soyer, T.; Turkmen, F.; Tatar, N.; Bozdogan, Oe; Kul, O.; Yagmurlu, A.; Cakmak, M.Choleithiasis is increasingly diagnosed in childhood and infancy. Biliary parasites are the rarest cause of cholelithiasis in all age groups. We present a twelve-year-old girl with non-hemolytic gallbladder stone and discuss the clinical features and differential diagnosis of Dicrocoelium dendriticum (DD) invasion that causes and/or mimics cholelithiasis in children.Öğe Sanguisorba minor subsp. Balearica inhibit production of cytokines in a chronic model of inflammation induced by complete Freund's adjuvant(Elsevier Ireland Ltd, 2019) Genc, A. I.; Adali, O.; Kul, O.; Ozkan, A. M. GenclerRheumatoid arthritis is an idiopathic auto-immune disease characterized destruction of cartilage and synovial membrane. TNF-alpha, IL-12 and IL-1 are well known inflammatory mediators of “rheumatoid arthritis”. These cytokines activate synoviocytes and chondrocytes and this results in the secretion of matrix metalloproteinase into the synovial fluid. Analgesic and anti-inflammatory drugs are used to suppress the symptoms. However, all these have several side effects. New therapies targeting the underlying immune process have been proposed. Recent research aims to discover long-acting anti-inflammatory drugs with minimal side effects. Sanguisorba minor subsp. Balearica (Smb) is known as a medicinal plant and has been used for treatment of various disorders. The usage of Smb for relieving of inflammation is noteworthy. This study aims to invastigate possible cytokine mediated anti-inflammatory effects of (Smb) on arthritic ratsÖğe Somatic SNPs of the BRCA2 gene at the fragments encoding RAD51 binding sites of canine mammary tumors(Wiley, 2017) Ozmen, O.; Kul, S.; Risvanli, A.; Ozalp, G.; Sabuncu, A.; Kul, O.Mammary tumors are the most common tumor type both in women and in female dogs. In women, heritable breast cancers have been linked mutations in the breast cancer susceptibility gene BRCA2 and it contains eight BRC repeats in exon 11 that bind to RAD51. In this study, we investigated the sequence variations of BRC1-BRC8 and C-terminus of canine BRCA2 gene. From a total of 64 canine patients with mammary tumors, 31 mammary tumors with benign and malign carcinomas and the 3 normal mammary glands were used for the study. In this study, 19 SNPs of exon 11 of BRCA2 in canine mammary tumors were detected for the first time. The c.2383A>C (T1425P) SNP was found to be the most probable disease-associated nsSNP. Our findings suggest that T1425P variation in BRC3 to be the most probable disease-associated nsSNP and may affect RAD51 binding strength.
