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Öğe Alteration of tissue expression of human beta defensin-1 and human beta defensin-2 in psoriasis vulgaris following phototherapy(Taylor & Francis Ltd, 2020) Uzunçakmak, Tuğba Kevser; Karadağ, Ayşe Serap; Özkanlı, Şeyma; Akbulak, Özge; Özlü, Emin; Akdeniz, Necmettin; Oğuztüzün, SerpilWe compared the expression profiles of antimicrobial peptides (AMPs) in psoriatic skin before and after narrow band ultraviolet B (nb-UVB) phototherapy and compared the levels to healthy controls. We studied 15 male and 12 female patients with psoriasis vulgaris, and 11 female and nine male control individuals. The patient group was treated with 24-36 sessions of nb-UVB phototherapy. Immunohistochemical staining for human beta defensin 1 (hBD-1) and human beta defensin 2 (hBD-2) expression of lesioned and control skin was performed prior to and following phototherapy. After phototherapy, the psoriatic area and severity index (PASI) decreased significantly in the treated patients compared to controls. The hBD-1 level was significantly higher in psoriasis patients than healthy controls. We found no statistically significant difference in hBD-1 and hBD 2 levels before and after phototherapy in the patient group. Although hBD-1 plays a role in psoriasis, levels of human beta defensin 1 and 2 are not affected significantly by phototherapy.Öğe Association Between TP53 Gene Polymorphism and Obesity(Society of Pharmaceutical Sciences of Ankara (FABAD), 2021) Ci?Han, Mehmethan; Buluş, Hakan; Di?Ri?Can, Onur; Oğuztüzün, Serpil; Öztürk, Doğan; Ünsal, Abdulkadir; Ada, Ahmet OğuzObesity is a chronic disorder with increasing prevalence worldwide and occurs when energy intake is greater than energy expenditure. Obesity is one of the factors that cause oxidative stress and arises from an imbalance between the reactive oxygen species (ROS) and the cell's antioxidant defense system. Increasing ROS in obesity, influencing the hypothalamic neurons, affects hunger and satiety control, so correspondingly on body weight control. When ROS amount increases, through DNA, protein and lipid oxidation, cell damage, necrosis, and apoptosis take place. Tumor protein p53, the guardian of the genome, is responsible for the regulation of genes involved in apoptosis as well as energy generating metabolic pathways. In our study, we investigated the TP53 (Arg72Pro) polymorphism in 151 patients diagnosed with obesity. TP53 mutation (rs1042522) was determined by real-time PCR. In 8 patients, the TP53 mutation was identified as carrying heterozygous (Arg72Pro) and in 143 patients carrying homozygous (wild type) (Arg72Arg). No individual with a homozygous mutant (Pro72Pro) genotype was found in the studied group. Associations between TP53 genotypes and clinical obesity parameters such as body mass index, thyroid stimulating hormon, glucose, postprandial blood sugar, triglyceride and cholesterol levels were compared statistically. According to the results of statistical analysis, it was observed that TP53 polymorphism was associated with insulin level. Genotype frequencies were also compared with previous studies performed in control populations and found to be different. This study shows that there may be a relationship between TP53(Arg72Pro) polymorphism and obesity. © 2021 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.Öğe Comparison and evaluation of CA 15-3, c-erbB-2, ER and PR tumor markers in pleural fluid cytology from metastatic breast cancer as a diagnostic tool(2009) Oğuztüzün, Serpil; Atay, Meral; Kiliç, Murat; ÖZhavzali, Müzeyyen; Atay, ZiyaThis study has been conducted to determine the diagnostic value of CA 15-3 in detecting metastatic breast carcinoma in pleural fluid. The material studied consisted of 115 malignant pleural effusions from invasive breast cancer patients. Expressions of CA 15-3, c-erbB-2, estrogen receptor (ER) and progesterone receptor (PR) were studied using immunocytochemistry. Of the cases studied, 115 were evaluated for CA 15-3, 70 for c-erbB-2, 100 for ER and 97 for PR. The 94% of the breast cancer cases studied showed a positive reaction with CA 15-3, while 31% for c-erbB-2, 20% for ER and 9% for PR were positive (p<0.01). The sensitivities of CA 15-3, c-erbB-2, ER and PR were 94%, 31%, 20% and 9%, respectively, for metastatic breast carcinoma. Our results indicate that CA 15-3 has the highest sensitivity for diagnosing malignant breast carcinomas in pleural fluids.Öğe Comparison of GST ısoenzyme expression in normal and neoplastic breast tissue: correlation with clinical and prognostic factors(2009) Oğuztüzün, Serpil; İşcan, Mesude; Özhavzalı, Müzeyyen; Sak, Serpil DizbayMeme dokusunda bulunan Glutatyon-S-transferazlar, meme tümörlerinin kemoterapiye verdiği cevapta ve karsinojenlerin mutajenik etkilerine duyarlılıkta önemlidir. Bu çalışmada, 43 invasif duktal kanser ve normal meme dokusunda glutatyon-S-transferaz enzimlerinin (alfa, mü, pi ve teta) immünohistokimyasal boyanma özellikleri araştırılmıştır. Ayrıca, GST izozimlerinin salınımları ve hastaların klinik özellikleri arasındaki ilişkiler de tespit edilmiştir. GST alfa, mü, pi ve teta izozimleri, vakaların % 100’ünde normal ve tümörlü meme dokularında farklı boyanma şiddetinde ve yaygın sitoplazmik olarak tespit edildi. GST alfa, mü ve pi enzimleri normal epitelde invasif tümör dokularına göre daha şiddetli boyanma göstermiştir (P 0,05). Ancak, istatistiksel olarak normal ve tümör epitel hücrelerinde GST teta salınımlarında bir fark tespit edilmemiştir (P 0,05). Bu çalışmada, mikrokalsifikasyon durumu ve GST mü; menapoz durumu ve GST alfa; tumor evresi ve GST mü salınımları arasında önemli bir ilişki gözlenmiştir (P 0,05). GST izozimleri ile estrojen reseptör durumu, evre, sigara içimi, kemoterapi, çocuk sayısı, yaş ve hormon terapi durumu arasında istatistiksel olarak bir ilişki bulunamamıştır (P 0,05).Öğe Endüstriyel kimyasallara maruz kalan işçilerin mesane kanserine yakalanma risklerinin değerlendirilmesi(2010) Oğuztüzün, Serpil; Kılıç, Murat; Tandoğan, Nisa; Öztürk, Latif; Gökbulut, Yazıcı ZuhalAMAÇ: Bu çalışmanın amacı, Kırıkkale’de endüstriyel kimyasallara maruz kalan işçilerin, mesane kanserine yakalanma risklerini idrar sitolojisi yöntemiyle değerlendirmektir. YÖNTEM: Barut fabrikasında çalışan ve yaş ortalamaları 475 yıl olan 63 işçinin idrar sitolojisi örnekleri, Papanicalaou yöntemiyle hazırlanmış ve ışık mikroskobunda değerlendirilmiştir. Sitolojik inceleme sonucunda kişilerin kimyasallara maruz kalma süreleri ve PAP sonuçları arasındaki ilişki Varyans Analiz (ANOVA) metoduyla %95 güvenilirlik düzeyinde istatistiksel olarak değerlendirildi. BULGULAR: Altmışüç işçiden kimyasallara maruz kalmayan 16 kişilik kontrol grubunun sitopatolojisi negatiftir. Kimyasallara maruz kalma süreleri 20 yıl ve üzerinde olan 47 kişiden 2’sinde metaplazik hücreler ve iki işçinin idrar sitoloji örneklerinde displazik hücreler tespit edilmiştir. Ayrıca, 30 yıl ve üzeri çalışan bir işçide ise ürotel karsinom görüldü. SONUÇ: Çalışma ortamında kimyasallara maruz kalma süresinin artmasıyla, mesane kanserine yakalanma riskinin de arttığı istatistiksel olarak anlamlı bulundu (p0,05).Öğe Evaluation of tissue levels of glutathione S-transferases (GST) isoenzymes in patients with discoid lupus erythematosus(2019) Yavuz, İbrahim Halil; Yavuz, Göknur Özaydın; Erten, Remzi; Bilgili, Serap Güneş; Karadağ, Ayşe Serap; Oğuztüzün, SerpilAim: Cutaneous lupus erythematosus (CLE) has a multifactorial pathogenesis involving genetic and environmental triggers and congenital and acquired immune response. The aim of this study was to investigate the effects of Glutathione S-Transferases (GST) isoenzymes including GSTT1, GSTM1, and GSTP1 in the CLE patients with an etiology of solar radiation exposure.Material and Methods: Paraffin-embedded skin biopsy sections from the patients were stained by immunohistochemical methods. The results were evaluated under a light microscope by a pathologist. The pattern, localization, and distribution of the immunohistochemical staining were recorded for each patient. Staining of the nucleus or cytoplasm was considered as positive staining. The accuracy of staining was determined based on the intensity and percentage of staining.Results: No significant difference was found between the patient and control groups regarding staining intensity. In terms of staining percentage, the prevalence of GSTP1-3 genotype was significantly lower in the patient group compared to the control group (25% vs. 63.33%) (p0.002).Conclusion: No significant difference was observed in the staining intensity of GSTP1, GSTT1, and GSTM1 between the patient and control groups and the staining percentage in some genotypes was even higher in the control group compared to the patient group.Öğe GSTM1, GSTP1, p53 as some probable predictors of prognosis in primary and metastatic epithelial ovarian cancer(2023) Özer, Gizem; Kaygın, Pınar; Dirican, Onur; Oğuztüzün, Serpil; Sarıaltın, Sezen Yılmaz; Şımşek, Gülçin Güler; Erdem, AyşegülObjectives: Ovarian carcinomas are responsible for the death of more women than all other gynecologic malignancies in the Western world. Ovarian carcinomas are detected in an advanced stage of the disease in approximately 80% of the patients. Glutathione S-transferases (GSTs) are an important family involved in the detoxification of several xenobiotics. Thus, this mechanism protects tissues from the harmful effects of oxidative stress and chemical-induced damages. The expression of them may contribute to the characteristics of ovarian carcinoma as they can metabolise both exogenous and endogenous compounds, which are implicated in the development of ovarian cancer. Therefore, our aim was to determine the expressions of GST Mu 1 (GSTM1), GST Pi 1 (GSTP1), and also p53, which is a tumor suppressor gene, in benign and malign ovarian tumors and metastasis tissues. Methods: A total of the 99 patients with ovarian tumor enrolled in the study. Thirty-one of the tissues was benign tumor, 17 was malign tumor and 51 was metastasis. The immunohistochemical GSTM1, GSTP1, and p53 staining characteristics of these tissues were investigated. Results: The highest GSTM1, GSTP1, and p53 expression was noted in the malignant group followed by the metastasis group. GSTP1 expression was significantly higher in malignant tissues than benign ones (p = 0.015). No statistically significant difference was observed in the level of GSTM1 expression between groups (p = 0.524). p53 expression was significantly higher in the metastasis and malignant tissues than the benign ones (p < 0.001). Conclusions: The higher expressions of GSTP1 and p53 in malignant and metastasis tissues than benign ones indicate that these expressions could be important biomarkers in ovarian cancer development and progression. Further studies with more cases are required to confirm the results of our present study.Öğe Immunocytolocalisations of cytokeratin 7 and 8 in primary and metastatic lung adenocarcinomas(Wiley, 2006) Oğuztüzün, Serpil; Melekoğlu, Adem…Öğe Immunohistochemical Evaluation of Apoptosis and Multidrug Resistance- Related Markers in Gallbladder Dysplasia and Carcinoma(2022) Başak, Kayhan; Demir, Derya; Koçdoğan, Arzu Kaya; Oğuztüzün, SerpilObjective: The search for treatment success in gallbladder carcinomas, which is one of the tumors with the most aggressive course, poor prognosis, and tendency to show resistance to treatment, continues today. Treatments targeting pathways related to genetic changes de- tected in most solid tumors offer new hope in the treatment of these tumors. Some of these treatment modalities target apoptosis-related pathways, and mammalian target of rapamycin (mTOR), p38, Bcl-2, and caspase-3 are important components of this pathway. Methods: In the study, mTOR, caspase-3, p38, Bcl-2, LL-37, MDR1, multidrug resistance protein (MRP)1, MRP6, and MRP7 immunohistochemical staining were applied to paraffin blocks of 27 gallbladder cancer and 62 cases with gallbladder dysplasia. The immunohisto- chemically stained sections were evaluated and scored. Results: mTOR, p38, and caspase-3 expressions were found to be significantly increased in dysplasia and tumor groups, and in dysplastic and malignant cells. While there was no signifi- cant difference in the expression of MRP1 and MRP7, MRP6 was significantly overexpressed. Conclusion: In this study, increased expression of mTOR, p38, and caspase-3 in the dys- plastic and malignant cells of the gallbladder may show that it has a role in the carcinogenesis process in the gallbladder. The study also shows that MRP6 may also play a role in the devel- opment of drug resistance in gallbladder carcinoma.Öğe Investigation of Glutathione S-Transferase-Alpha and Glutathione S-Transferase-Pi Expression Levels in Spermophilus xanthoprymnus and Meriones tristrami in Terms of Living Conditions and Natural Habitat Differences in Kırıkkale Province(2023) Pamukoğlu, Nahit; Oğuztüzün, Serpil; Dirican, Onur; Sarıaltın, Sezen YılmazGlutathione S-transferase (GST) is a multifunctional enzyme that provides homeostasis by catalyzing the first step in the formation of the end product mercapturic acid in the detoxification metabolic pathway. Being found in mammals, insects, fish, birds, annelids, molluscs, and many microorganisms, GST takes part the elimination of toxic substances taken into body by consuming food, and their transport by binding non-substrate ligands (e.g. heme and bilirubin) with GSH. In addition, it can prevent reactive electrophilic compounds from harming the body by covalent bonding similar compounds to each other. These xenobiotic acceptors affected by GST include nitrogen halogen compounds, organophosphates, and polycyclic aromatic hydrocarbons. Xenobiotics are oxygenated by this enzyme system, the next mechanism of oxygenated products is more oxygenation, and these products become more easily soluble in water. In this study, Glutathione S-Transferase was detected in the liver tissue of Spermophilus xanthoprymnus and Meriones tristrami and its characteristic features were determined. For this purpose, the animals were anesthetized with sodium pentobarbital and their liver tissues were harvested. After necessary preparations were completed, the samples were analyzed by using immunohistochemical staining method and the expressions of GST isozymes were determined. As a result, glutathione s-transferase-alpha and glutathione s-transferase-pi expression levels were found to differ in Spermophilus xanthoprymnus and Meriones tristrami samples obtained from different localities of Kırıkkale province. Differences in GST enzyme expression in these species indicate that both species differ in their detoxification capacity and response to xenobiotics.Öğe Investigation of GSTM1 and GSTT1 polymorphisms in obesity patients under bariatric surgery(Society of Pharmaceutical Sciences of Ankara (FABAD), 2021) Ünsal, Abdulkadir; Buluş, Hakan; Dirican, Onur; Oğuztüzün, Serpil; Öztürk, Doğan; Cihan, Mehmethan; Ada, Ahmet OğuzObesity is a chronic disorder with increasing prevalence worldwide and occurs when energy intake is more than energy expenditure. Obesity is one of the factors that cause oxidative stress and arises from an imbalance between the reactive oxygen species ROS and the cell’s antioxidant defense system. Increasing ROS in obesity, influencing the hypothalamic neurons, affect hunger and satiety control, so correspondingly on body weight control. When ROS amount increases, through DNA, protein, and lipid oxidation, cell damage, necrosis, and apoptosis take place. Oxidative stress increment in adipose tissue causes the development of metabolic syndrome in obese people. Also, weight loss due to calorie restriction or exercise reduces oxidative stress. Mitochondria is the essential source for ROS formation. In the electron transfer system, reactive oxygen species forming due to oxidative phosphorylation reactions are involved in various physiological processes such as cell proliferation and differentiation. Glutathione S-transferase M1 and T1 genes encode enzymes that have oxidant-scavenging activities. Deletion polymorphisms in these genes cause the absence of their corresponding enzymes. In this study, we investigated the parameters associated with obesity such as body mass index (BMI), TSH, glucose, satiety blood glucose, triglyceride, and cholesterol levels, and deletion polymorphisms of GSTM1 and GSTT1 genes in 152 patients diagnosed with obesity in a Turkish population. No statistically significant relationship was found between the parameters studied in obese patients and GSTM1 and GSTT1 polymorphisms. More studies are needed to elucidate the relationship of GSTM1 and GSTT1 polymorphisms with obesity. © 2021 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.Öğe Küçük Hücreli Dışı Akciğer Kanserinde Glutatyon S-Transferaz M1 ve T1 Polimorfizmleri ve Protein İfadeler(2017) Kılıç, Murat; Ada, Ahmet Oğuz; Oğuztüzün, Serpil; Demirağ, Funda; Çelik, Sezgin; Bıçakcıoğlu, Pınar; İşcan, MümtazAmaç: Glutatyon S-transferaz (GST) GSTM1 ve GSTT1 genlerinde delesyon polimorfizmleri, karsinojenlerin detoksifikasyonunu azaltan ilgili enzimlerin yokluğuna neden olur. Aynı hastalarda polimorfizm ve protein ifadelerinin değerlendirildiği çalışmalar sınırlıdır. Bu nedenle, bu çalışmada, küçük hücreli dışı akciğer kanser (KHDAK) hastalarının akciğer dokularında, GSTM1 ve GSTT1 polimorfizmleri ile protein ifadeleri arasındaki ilişkinin incelenmesini amaçladık.Gereç ve Yöntemler: Protein ekspresyon ve gen delesyon çalışmaları için, 33 KHDAK'li hastanın arşiv dokularından elde edilen tümörlü ve çevresindeki normal doku çiftleri kullanıldı. Parafine gömülü dokularda, protein ekspresyonlarını belirlemek için immünohistokimyasal metod, gen delesyonlarını belirlemek için multipleks polimeraz zincir reaksiyonları yöntemi kullanıldı. Bulgular: GSTM1 ve GSTT1 gen delesyonlarına sahip hastalarda GSTM1 ve GSTT1 protein ifadeleri bulunmazken, GSTM1 ve GSTT1 genlerini taşıyan tüm hastaların akciğer dokularında protein ifadeleri saptanmıştır. GSTT1'in protein ifadesi, GSTM1 geninden yoksun olan hastaların tümünde, GSTM1 genine sahip olanlardan 2.0 kat daha yüksek olduğu gözlenmiştir (p=0.018). GSTM1'in protein ifadesi, GSTM1 genine sahip hastaların tümör dokularında, normal dokularından istatistiksel olarak daha yüksek belirlenmiştir (p=0.001). Sonuç: Bu sonuçlar, a) KHDAK'li hastaların GSTM1 ve GSTT1 protein ifadeleri ile gen delesyonları arasında bir ilişkinin olduğunu, b) GSTM1 geninin yokluğunda, tümör dokularında GSTT1 protein ifadesinin artışı bu dokularda GSTT1'in toksik elektrofilikleri detoksifiye etme kapasitesini arttırma eğiliminde olduğunu ve c) KHDAK'li hastaların normal dokularına kıyasla tümörlü dokularında GSTM1 protein ifadesinin daha yüksek olduğunu göstermektedir.Öğe MCF-7 İnsan Meme Kanseri Hücre Soyunda Doksorubisin Öncesi ve Sonrası GST İzozimlerinin, İlaç Dirençlilik Proteinlerinin ve Apoptotik Etkisinin Araştırılması(2020) Koçdoğan, Arzu Kaya; Oğuztüzün, Serpil; Şımşek, Gülçin Güler; Türk, MustafaAmaç: Kanser hastalığının tedavisinde karşılaşılan klinik sorunlardan biri hastalara uygulanan kemoterapiye karşı tümör hücrelerinin geliştirdiği dirençtir. Tümör hücrelerinin ilaçlara karşı gösterdiği direncin asıl kaynaklarından biri, ilaçların hücre dışına atılmasını sağlayan membran proteinlerinin en önemli üyelerinden ABC (ATP-binding cassette) taşıyıcı proteinleridir. İlaç dirençlilik proteinlerinin yanında diğer hücre içi proteinlerinde etkin olabileceği bilinmektedir. Bu bağlamda, alkilleyici özellikteki kanser ilaçlarına gelişen dirençte, hücre içi glutatyon ve glutatyon S- konjugatlarının seviyelerinin artmasının rolünün olduğu bildirilmiştir. Bu çalışmada, Michigan Cancer Foundation-7 (MCF-7) meme kanseri hücre hattında Glutatyon S- transferaz (GST) izozimlerinin çoklu ilaç direnç mekanizmasındaki bazı önemli proteinlerin doksorubisin uygulamasıyla ilişkilerinin incelenmesi amaçlanmıştır. Yöntem: Bu çalışmada ilaç uygulanmış ve uygulanmamış meme kanserli MCF-7 hücre hattında GST enzim ailesi ve ABC taşıyıcı proteinlerin ekspresyon ifadeleri immünositokimya yöntemiyle incelenmiştir. Bulgular: Bu çalışmada, ilaç uygulanmış hücre hatlarında $GSTP_1, GSTT_1, GSTM_1, GSTA_1, GSTO_1, GSTZ_1 ve GSTK_1$ protein ifadelerinin ilaç uygulanmamış hücrelere oranla daha yüksek olduğu görülmüştür; $GSTS_1$ proteini kontrol ve deney gruplarının ikisinde de tespit edilememiştir. İlaç uygulanmış MCF-7 hücre hattında MRP (Multidrug resistance-associated) 2,3,6,7 protein ifadelerinin ilaç uygulanmamış hücrelere oranla daha fazla olduğu görülmüştür. MDR1 (multidrug resistance protein) ve MRP1 proteinleri izlenememiştir. İlaç uygulanmış meme kanserli MCF-7 hücre hattında Bcl-2, p53, p38, caspase-3 protein ifadelerinin ilaç uygulanmamış hücrelere oranla daha fazla olduğu görülmüştür. Sonuç: ABC süper ailesi üyelerinden MRP 2,3,6 ve 7 ile Faz II enzimleri arasında bulunan $GSTP_1, GSTT_1, GSTM_1, GSTA_1, GSTO_1, GSTZ_1 ve GSTK_1$ izozimlerinin, MCF-7 kanser hücre hattında doksorubisine karşı oluşan ilaç dirençliliğinde rolleri olduğu belirlenmiştir.Öğe Subepithelial Fibrinous Accumulation and Associated Epithelial Proliferation in Laryngeal Nodules(2022) Başak, Kayhan; Günhan, Ömer; Çaputcu, Merve; Arda, Şule Sağlam; Atlı, Muharrem; Demir, Derya; Oğuztüzün, SerpilObjective: Fibrinoid accumulation in the larynx and increase in the subepithelial collage-\rnous connective tissue result in overgrowth. Mucosal epithelium may proliferate downward\rto organize and remove the fibrinoid accumulation. This downward proliferation may cause\ran invasive cancer-like image. This study focused on the pathogenesis of the accumulation of\rfibrinoid substance and the development mechanism of the associated squamous epithelium\rproliferation.\rMethods: Five hundred and seventy-five laryngeal nodules were reexamined and 111 of\rthem with varying degrees of irregular downward squamous epithelial proliferation were\rincluded in the study. Immunohistochemical staining of CK5/6, CK17, CK14, collagen type\rI, collagen type III, collagen type IV, and fibrinogen was performed. A modified Masson’s\rtrichrome method was used for the histochemical staining of collagen.\rResults: Edema was present in 18% of the acute lesions and fibrin deposition in 42%. Rela-\rtively mature lesions mostly contain dense collagen fibers. The intensity of collagen type III\rwas inversely proportional to the amount of fibrin accumulation. Collagen type IV was found\rin the epithelial and vascular basement membranes. A decrease in fibrin staining intensity\rand the presence of collagen type I and type III indicated the replacement of fibrin with col-\rlagen. Basal-type keratins showed more pronounced staining in the regenerated areas of the\repithelium. As the laryngeal subepithelial fibrinoid accumulation was replaced with collagen,\rregression of the lesion became difficult.\rConclusion: Irregular squamous epithelial proliferation occurs independent of the stage of\rthe lesion. Although the etiology is different, the resulting lesions are histologically similar to\rthose seen in the ligneous mucosal diseaseÖğe Unveiling the etiological impact of GST-M1, GST-T1, and P53 genotypic variations on brain carcinogenesis(Springer Science and Business Media B.V., 2024) Dirican, Onur; Kaygın, Pınar; Oğuztüzün, Serpil; Husseini, Abbas Ali; Sarıaltın, Sezen Yılmaz; Yılmaz, Can; Ünlü, NihanBackground: Functional variants of glutathione-S-transferase (GST)-M1, GST-T1, p53 might modulate brain cancer risk by altering the rate of metabolism and clearance of carcinogens from the brain tissue. In this study, the role of GST-M1, GST-T1, p53 polymorphisms on brain tumor was investigated. Methods and results: Brain tumor tissues of 143 patients were obtained from the Gulhane Training and Research Hospital, Department of Neurosurgery between 2019 and 2020. In the xenobiotic mechanism, the null allele frequency in the GST-T1, GST-M1 gene regions of Phase II enzymes by qPCR method were investigated. Single nucleotide polymorphism encoding Arg/Pro conversion in the p53 gene region was analyzed in 120 cases by sequence analysis method. The data were analyzed statistically with patient’s demographic and clinical data. GST-M1, GST-T1, p53 genotypes of the patient group were determined. The most frequent genotype was null genotype (0/0) for GST-M1 (?2 = 39.756, p < 0.001). GST-M1 genotype frequencies were 30.8%, 23.1%, 44.3% for 1/1, 1/0, 0/0, respectively. The most frequent genotype was GST-T1 1/1 following by GST-T1 1/0 (?2 = 0.335, p = 0.846). GST-T1 genotype frequencies were 64.3%, 30.8%, 4.9% for 1/1, 1/0, 0/0, respectively. GST-M1 null genotype might be associated with the development of brain tumors. Genotype distribution obtained in p53 exon 4 codon 72; Arg/Arg was determined as 31 (25.8%), Arg/Pro 70 (58.3%), and Pro/Pro 19 (15.8%) in the case group, while there were 18 (38.3%), 23 (48.9%), and 6 (12.8%) respectively in the control group. However, the genotype distribution of p53 exon 4 codon 72 among tumorous tissue did not significantly vary from healthy control tissues (?²=2.536, p = 0.281). Conclusion: The null allele frequency encountered in the GST-M1, GST-T1 gene regions is consistent with the rates in the gene pool called Caucasian in the literature. GST-M1 gene polymorphism may play a crucial role in brain carcinogenesis in Turkish patients. This study based on clinical data is thought to help to understand the important epidemiological features of brain tumors. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.
