Yazar "Sengun, Abdulkadir" seçeneğine göre listele

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    Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis
    (Japanese Soc Dental Materials Devices, 2015) Malkoc, Meral Arslan; Demir, Necla; Sengun, Abdulkadir; Bozkurt, Serife Buket; Hakki, Sema Sezen
    To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tukey-Kramer tests. BQM, RU200, and ME demonstrated a significant decrease in the bDPCs' index at 24 and 72 h (p <= 0.001). These materials were found to be the most toxic resin cements, as compared to the control and other tested materials (C2 and MA). However, C2 and MA showed a better survival rate, compared to BQM, RU200, and ME, and had lower cell index than the control group. The cytotoxic effects of resin cements on pulpa should be evaluated during the selection of proper cements.
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    Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysis
    (Korean Acad Prosthodontics, 2015) Malkoc, Meral Arslan; Demir, Necla; Sengun, Abdulkadir; Bozkurt, Serife Buket; Hakki, Sema Sezgin
    PURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 mu L of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P<.001). When the cells were exposed to media by Ultratemp, the cell viability was similar to that of the control at 24 hours (P>.05); however, the cell viability was significantly reduced at 72 hours (P<.001). Light and scanning electron microscopy examination confirmed these results. CONCLUSION. The cytotoxic effects of temporary cements on pulpal tissue should be evaluated when choosing cement for luting provisional restorations.
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    Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells
    (Hindawi Publishing Corporation, 2013) Ulker, Hayriye Esra; Ulker, Mustafa; Gumus, Hasan Onder; Yalcin, Muhammet; Sengun, Abdulkadir
    This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells (P < 0.05). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test (P > 0.05). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.
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    Dental approach to erosive tooth wear in gastroesophageal reflux disease
    (Makerere Univ, Fac Med, 2014) Dundar, Ayse; Sengun, Abdulkadir
    Background: The duration of gastro-esophageal reflux disease (GERD), the frequency of reflux, the pH and type of acid, and the quality and quantity of saliva affect the severity of dental erosion due to GERD. Objective: To summarize the diagnostic protocol and treatment of dental erosion due to GERD. Methods: A Medline literature search was performed to identify articles associated with a dental approach to GERD. Results: The dental professional must carry out a diagnostic protocol, which includes collecting data on the patient's medical and dietary history, occupational/recreational history, dental history, and oral hygiene methods. Intraoral, head and neck, and salivary function examinations should be performed to expose the dental implications of GERD symptoms. Conclusion: Diagnosing the cause of erosive tooth wear can help prevent further damage. Patients must be informed about how to prevent GERD.
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    Effect of accelerated aging on the bonding performance of fluoridated adhesive resins
    (Japanese Soc Dental Materials Devices, 2014) Cobanoglu, Nevin; Ozturk, Bora; Sengun, Abdulkadir; Cetin, Ali Riza; Ozer, Fusun
    The purpose of this study was to investigate the dentin bond durability of a one-step, fluoride-containing, glass ionomer-based adhesive system, Reactmer Bond (RB), and that of a two-step, fluoride-containing, self-etch adhesive system, Clearfil Protect Bond (CPB). Enamel was removed from the occlusal surfaces of teeth, and flat dentin surfaces were entirely covered with a composite resin following the application of an adhesive material (n=10). After specimens were sectioned into rectangular sticks of 0.87 +/- 0.03 mm(2), the sticks were randomly assigned into two accelerated aging time period groups: 1 week or 1 year. Microtensile bond strengths were determined. Bond strength of RB increased significantly after 1 year (1 week=27.80 +/- 10.57 MPa versus 1 year=36.93 +/- 14.38 MPa) (p<0.05). In contrast, there was no significant difference in bond strength between the two time periods for CPB (1 week=51.74 +/- 17.8 MPa versus 1 year=56.03 +/- 18.85 MPa) (p>0.05). Both fluoride-containing adhesives seemed to demonstrate reliable bonding performance after 1 year of accelerated aging in water.
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    Human and bovine pulp-derived cell reactions to dental resin cements
    (Springer Heidelberg, 2012) Ulker, Hayriye Esra; Hiller, Karl Anton; Schweikl, Helmut; Seidenader, Claudia; Sengun, Abdulkadir; Schmalz, Gottfried
    Objectives The objective of this study was to evaluate the cytotoxic reaction of transfected human pulp derived cells (tHPDC) and transfected bovine pulp derived cells (tBPDC) after exposure to resin cements [RelyX UnicemClicker (RX), MaxCem (MC), Panavia F 2.0 (PF), BisCem (BC), and Bistite II DC (BII)] and to compare it to the generation of reactive oxygen species (ROS). Materials and methods Set materials were extracted in culture medium, cell survival as a measure of cytotoxicity was determined photometrically using crystal violet after cells were exposed to the extracts for 24 h. The generation of ROS was detected by flow cytometry after cells were exposed to extract dilutions for 1 h. Results The ranking of the least to the most cytotoxic material was: RX < BII < PF < BC < MC for both cell lines, but for tHPDC, only MC and PF eluates were different from untreated controls. Generally, tBPDC were more susceptible to materials than tHPDC, but only for RX and BC was this difference statistically significant. All undiluted extracts increased ROS production in both cell lines but to a higher amount in tHPDC than in tBPDC. Conclusions tHPDC reacted less sensitive than tBPDC in the cytotoxicity test but with the same rank order of materials. In contrast, the cellular oxidative stress reaction was more pronounced in tHPDC than in tBPDC. Clinical relevance Depending on the residual dentine layer in deep cavities, biologically active resin monomers or additives released from resin cements may influence the dentine-pulp complex, for instance, its regenerative and reparative capacities.