Human and bovine pulp-derived cell reactions to dental resin cements
Yükleniyor...
Tarih
2012
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Heidelberg
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objectives The objective of this study was to evaluate the cytotoxic reaction of transfected human pulp derived cells (tHPDC) and transfected bovine pulp derived cells (tBPDC) after exposure to resin cements [RelyX UnicemClicker (RX), MaxCem (MC), Panavia F 2.0 (PF), BisCem (BC), and Bistite II DC (BII)] and to compare it to the generation of reactive oxygen species (ROS). Materials and methods Set materials were extracted in culture medium, cell survival as a measure of cytotoxicity was determined photometrically using crystal violet after cells were exposed to the extracts for 24 h. The generation of ROS was detected by flow cytometry after cells were exposed to extract dilutions for 1 h. Results The ranking of the least to the most cytotoxic material was: RX < BII < PF < BC < MC for both cell lines, but for tHPDC, only MC and PF eluates were different from untreated controls. Generally, tBPDC were more susceptible to materials than tHPDC, but only for RX and BC was this difference statistically significant. All undiluted extracts increased ROS production in both cell lines but to a higher amount in tHPDC than in tBPDC. Conclusions tHPDC reacted less sensitive than tBPDC in the cytotoxicity test but with the same rank order of materials. In contrast, the cellular oxidative stress reaction was more pronounced in tHPDC than in tBPDC. Clinical relevance Depending on the residual dentine layer in deep cavities, biologically active resin monomers or additives released from resin cements may influence the dentine-pulp complex, for instance, its regenerative and reparative capacities.
Açıklama
Joint Meeting of the Continental-European-Division, Scandinavian-Division and Israeli-Division of the International-Association-for-Dental-Research (IADR) -- SEP 10-12, 2009 -- Munich, GERMANY
SENGUN, Abdulkadir/0000-0002-6053-160X
SENGUN, Abdulkadir/0000-0002-6053-160X
Anahtar Kelimeler
Cytotoxicity, Resin cements, Reactive oxygen species, Human pulp-derived cells, Bovine pulp-derived cells
Kaynak
Clinical Oral Investigations
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
16
Sayı
6
Künye
closedAccess
