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Öğe Alterations in some clinical biochemistry values of Honamli and Native Hair goats during pubertal development(Univ Zagreb Vet Faculty, 2015) Devrim, Alparslan K.; Elmaz, Ozkan; Mamak, Nuri; Sudagidan, MertHonamli goats have been defined and recorded as a new breed but the literature is very limited on these goats. In terms of meat production, they have been reported as having some of the highest potential among the goat breeds in Turkey. The aim of the present study was to determine the alterations in blood serum chemistry values in the puberty period in Honamli and Native Hair goats. Selected biochemistry parameters were chosen due to their association with meat production potential. Blood samples of Honamli (n = 90, 45 animals for each sex) and Native Hair goats (n = 90, 45 animals for each sex) were obtained from goat herds in the Western Mediterranean region of Turkey. The values of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate transaminase (AST), creatine kinase (CK), calcium, cholesterol, creatinine, iron, magnesium, phosphorus, total lipids, triglycerides and uric acid were measured in the sera obtained from blood samples of males and females in equal numbers of each species, in the three age groups of 4, 8 and 12 months. One-way analysis of variance and the Tukey test were performed for statistical evaluation. In both male and female animals, ALT, AST, CK, calcium, cholesterol, creatinine, iron, magnesium, phosphorus, total lipids, triglycerides and uric acid levels exhibited significant (P<0.05) differences between the age groups. Our findings suggest that there are age-associated parallel increases of ALT values in female goats and total lipid values in male goats. The present study also reveals that the analyzed biochemical values usually change after 4 months of age when compared with the other age groups.Öğe An Investigation on the Dna Binding Activities of Melamine, Cyanuric Acid and Uric Acid(Editura Acad Romane, 2021) Şenol, Ali; Devrim, Alparslan Kadir; Sudagidan, Mert; Özalp, Veli CengizMelamine can be added to various foods such as milk, milk powder, baby food, pet, and livestock feed for cheating purposes due to its high nitrogen content. Regarding its usage in food products, there is a need to investigate its possible interactions with DNA. Thus, this study aimed to investigate the interactions of melamine and its metabolized products, cyanuric acid and uric acid with genomic DNA, isolated from eukaryotic (calf thymus) and prokaryotic (Staphylococcus aureus) sources. UV-absorbance spectrophotometry, fluorescence spectrophotometry, and agarose gel electrophoresis techniques were used to evaluate these interactions. The five different concentrations of melamine, cyanuric acid, and uric acid were incubated with fixed DNA concentration and it was determined that the test compounds interacted with the DNA molecules. The data obtained by UV-absorbance and fluorescence spectrophotometry techniques revealed an increase in wave peaks observed with the increasing substance concentration. After the obtained data of the aforementioned techniques were evaluated together, it was concluded that melamine, cyanuric acid, and uric acid bonded to the eukaryotic and prokaryotic genomic DNA materials via groove binding.Öğe COMPARISON OF SOME COPPER COMPOUNDS IN TERMS OF THEIR INTERACTIONS WITH DNA USING AGAROSE GEL ELECTROPHORESIS, UV-ABSORBANCE AND FLUORESCENCE SPECTROPHOTOMETRY TECHNIQUES(Malaysian Society for Biochemistry and Molecular Biology, 2023) Akanbong, Elisha Apatewen; Devrim, Alparslan Kadir; Şenol, Ali; Sudagidan, MertIn this study, to investigate the interaction of genomic DNA with some copper compounds (CuSO4, CuCO3, and CuCl2) at various concentrations (1000 ?M, 500 ?M, 250 ?M, 125 ?M and 62.5 ?M); UV-absorbance spectrophotometry, agarose gel electrophoresis, and fluorescence spectrophotometry techniques were used. When the UV-spectrophotometry data were examined within the wavelength range of 220-320 nm, the hyperchromic effect of CuCl2 was evaluated to be proportional to its concentrations. Within the wavelength range, the copper compounds produced their strongest hyperchromic effect on the DNA at 1000 ?M. When the fluorescent spectrophotometry data were evaluated, the hypochromic effect of CuCl2 within the wavelength range of 400-700 nm was determined at 1000 ?M. CuSO4 showed a hypochromic effect at concentrations above 62.5 ?M. Also, it was observed that CuCO3 did not interact with DNA. According to the agarose gel electrophoresis findings, the copper compounds investigated decreased DNA band intensity as their concentrations increased. It was observed that the most significant decrease in band intensity was caused by CuCl2 at 1000 ?M. Again, it was observed that the copper compounds did not cause any cleavage in the genomic DNA within the concentration range 62.5-1000 ?M. Consequently, the copper compounds bind to DNA, most probably by non-intercalative mode. In this regard, they could have the potential to be used in the development of new therapeutic agents. Thence, conducting further studies on the interaction of the copper compounds with DNA, their possible DNA uncoiling activities, and also the investigation of these compounds in cancer cell lines will provide useful results. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.Öğe DNA-METAL INTERACTION AND THE BIOLOGICAL ACTIVITIES OF METAL COMPLEXES: AN OVERVIEW IN THE LIGHT OF RECENT LITERATURE(Editura Acad Romane, 2021) Senol, Ali; Akanbong, Elisha Apatewen; Sudagidan, Mert; Devrim, Alparslan KadirDeoxyribonucleic acid (DNA), which leads the biological synthesis of proteins and enzymes in living cells and carries genetic information, is a potential target for binding many molecules such as metals, metal complexes, anticancer drugs, and some harmful chemicals. Molecules that bind to DNA by covalent or non-covalent intercalation, electrostatic interaction, and groove binding cause disruption in the double-stranded DNA structure. This situation is of great importance in studies conducted in the field of pharmacy to increase the effectiveness of antitumor and antiviral drugs, in DNA-based disease diagnosis and determination of toxicity properties of metal complexes. The purpose of this review is to evaluate the interactions of DNA-metal complexes and provide information about their determined biological activities.Öğe The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity(Humana Press Inc, 2018) Sozmen, Mahmut; Devrim, Alparslan K.; Kabak, Yonca B.; Devrim, Tuba; Sudagidan, MertPeriostin is an extracellular matrix protein from fasciclin family, and it plays an important role in the cell adhesion, migration, and growth of the organism. Periostin prevents apoptosis while stimulating cardiomyocytes. The present study was designed to investigate cardioprotective effects of the recombinant murine periostin peptide administration in a rat model of isoproterenol (ISO)-induced myocardial injury. The experiment was performed on 84 adult male Sprague Dawley rats in 4 groups (n = 21): control group (1), periostin-treated group (2), ISO-treated group (3), and ISO + periostin-treated group (4). The groups were further divided into three subgroups based on the duration of the experiment in which rats were killed on days 1, 7, and 28 (n = 7). Growth factors (VEGF, ANGPT, FGF-2, TGF beta), mitosis and apoptosis (Bcl-2, Bax, PCNA, Ki-67, phospho-histone H3), cell cycle activators and inhibitors (cyclin D1, D2, A2, Cdc2), the origin of regenerating cells (cKit and CD45) mRNA were detected using quantitative real-time polymerase chain reaction (PCR) and PCR array. Immunohistochemistry staining was used to directly detect protein level and distribution in the heart tissues. Administration of periostin following ISO-induced cardiotoxicity revealed that periostin alleviated deleterious effects of ISO through several pathways: (1) periostin induced mitotic activity of endothelial/fibroblastic cells, (2) periostin drives cardiomyocytes into S and M phases, thus promoting proliferation of cardiomyocytes, (3) periostin contributed to collagen degradation, tissue remodeling, and reduced cardiac fibrosis during the healing process following myocardial damage while preserving tissue matrix, (4) periostin stimulated angiogenesis by upregulating THBS1, TGFB2, and HGF genes, (5) periostin regulated cell growth and proliferation while maintaining cell shape and cellular muscle contractions (ACTB) and functioned as chemoattractant factor (CCL2) at the beginning of myocardial damage.Öğe Expression levels of angiogenic growth factors in feline squamous cell carcinoma(AKADEMIAI KIADO ZRT, 2020) Kabak, Yonca B.; Sozmen, Mahmut; Devrim, Alparslan K.; Sudagidan, Mert; Yildirim, Funda; Guvenc, Tolga; Yarim, MuratSquamous cell carcinoma (SCC) is the most common malignant neoplasm of the skin in cats. Tumour angiogenesis is the pivotal event for tumour progression and metastasis. We assessed protein and gene expression of angiogenic growth factors including bFGF, VEGF-C, TGF-beta, PDGF-A, PDGF-C and PDGFR-alpha that possibly contribute to the angiogenic phenotype of feline SCC (FSCC) and could, therefore, be a good target in the treatment of SCC. In the present study, a total of 27 FSCC cases were investigated. Tumour cases were histopathologically classified as well differentiated (10/27), moderately differentiated (5/27), and poorly differentiated (12/27). The expression levels of the growth factors were detected using immunohistochemistry and assessed semi-quantitatively. Growth factor expression levels were evaluated at different locations: in the oral region, in areas exposed to solar UV radiation including the ears, eyelids and nasal planum, and other miscellaneous locations. Our findings have revealed that FSCC arising from different anatomical sites of the body and showing differences in aggressiveness, metastasis, and prognosis may be angiogenesis dependent, and angiogenic key regulators could play a role in the development of FSCC.Öğe The effect of krill oil on Wnt/ß-catenin signaling pathway in acetaminopheninduced acute liver injury in mice(Walter De Gruyter Gmbh, 2023) Şahin, Yaşar; Devrim, Alparslan Kadir; Alcigir, Mehmet Eray; Şenol, Ali; Ekici, Hüsamettin; Devrim, Tuba; Sudagidan, MertObjectives: This study investigated the effect of krill oil (KO) on liver damage caused by acetaminophen (APAP). Methods: In the present study, the control and APAP groups were given distilled water by gavage for 14 days. In addition, the KO and APAP+KO groups were given 500 mg/kg krill oil by gavage for 14 days. At the end of 14 days, 0.9 % sodium chloride solution (saline solution) administration was applied intraperitoneally to the control and KO groups. Meanwhile, 220 mg/kg acetaminophen was administered to the APAP and APAP+KO groups. While some biochemical parameters in plasma were examined, some oxidative stress parameters in plasma and liver tissue were evaluated. Apoptotic and inflammatory responses of some primer sequences determined by quantitative Real-Time PCR (qPCR) in liver tissue. After histopathological examination of liver tissue, immunohistochemical analysis was performed with Wnt inhibitory factor-1 (Wif-1), beta-catenin (ss-Catenin), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Results: The Wif-1 positivity in hepatocytes increased significantly in the APAP group (5.29 +/- 0.71) compared to the control (1.14 +/- 0.51), and KO (2.14 +/- 0.55) groups (p<0.001). The 8-OHdG positivity in hepatocytes increased significantly in the APAP group (19.57 +/- 0.58) compared to the control (0.43 +/- 0.20), KO (3.57 +/- 0.48), and APAP+KO (4.00 +/- 2.53) groups (p<0.001). Conclusions: As a result, krill oil could be used as a nutritional supplement to protect the liver against acetaminopheninduced liver injury.
