Yazar "Tandogan, Nisa" seçeneğine göre listele

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    Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells
    (Via Medica, 2010) Ada, Kezban; Turk, Mustafa; Oguztuzun, Serpil; Kilic, Murat; Demirel, Mehmet; Tandogan, Nisa; Latif, Ozturk
    The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 mu g/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 mu g/mL concentration for 16 h, but high in 400-500 mu g/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 mu g/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.
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    Usefulness of CA 15-3 for breast or ovarian primary sites in metastatic adenocarcinoma of pleural fluid
    (Tubitak Scientific & Technical Research Council Turkey, 2012) Oguztuzun, Serpil; Kilic, Murat; Tandogan, Nisa; Ozturk, Latif
    This study was conducted to evaluate the diagnostic value of CA 15-3, mammaglobin, c-erbB-2, estrogen receptor (ER), progesterone receptor (PR), CK 20, and CA 125 for detecting metastatic breast and ovarian carcinoma in pleural fluids. The material under study consisted of 26 pleural effusions from invasive breast and ovarian cancer patients from the Hannover Cytopathology Institute. Expressions of these markers were studied using immunocytochemistry. All of the breast cancer cases studied showed a positive reaction with CA 15-3, while only 36% with mammaglobin, 21% with c-erbB-2, 36% with ER, and 7% with PR were positive. The sensitivities of CK 20, CA 15-3, and CA 125 as markers for metastatic ovarian carcinoma were 43%, 88%, and 55%, respectively. Our results indicate that CA 15-3 has a higher sensitivity than other tumor markers for diagnosing metastatic breast and ovarian carcinoma in pleural fluids.