Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells
Yükleniyor...
Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Via Medica
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 mu g/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 mu g/mL concentration for 16 h, but high in 400-500 mu g/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 mu g/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.
Açıklama
Kilic, Murat/0000-0002-1377-2021
Anahtar Kelimeler
NiO nanoparticles, cancer, toxicity, apoptosis
Kaynak
Folia Histochemica Et Cytobiologica
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
48
Sayı
4
Künye
Ada, K., Turk, M., Oguztuzun, S., Kilic, M., Demirel, M., Tandogan, N.S., Ersayar, E., & Latif, O. (2011). Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells. Folia histochemica et cytobiologica, 48 (4), 524-529 .
