Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells

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dc.contributor.authorAda, Kezban
dc.contributor.authorTurk, Mustafa
dc.contributor.authorOguztuzun, Serpil
dc.contributor.authorKilic, Murat
dc.contributor.authorDemirel, Mehmet
dc.contributor.authorTandogan, Nisa
dc.contributor.authorLatif, Ozturk
dc.date.accessioned2020-06-25T17:51:22Z
dc.date.available2020-06-25T17:51:22Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.descriptionKilic, Murat/0000-0002-1377-2021
dc.description.abstractThe aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 mu g/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 mu g/mL concentration for 16 h, but high in 400-500 mu g/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 mu g/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.en_US
dc.identifier.citationAda, K., Turk, M., Oguztuzun, S., Kilic, M., Demirel, M., Tandogan, N.S., Ersayar, E., & Latif, O. (2011). Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells. Folia histochemica et cytobiologica, 48 (4), 524-529 .en_US
dc.identifier.doi10.2478/v10042-010-0045-8
dc.identifier.endpage529en_US
dc.identifier.issn0239-8508
dc.identifier.issn1897-5631
dc.identifier.issue4en_US
dc.identifier.pmid21478093
dc.identifier.scopus2-s2.0-79958041876
dc.identifier.scopusqualityQ2
dc.identifier.startpage524en_US
dc.identifier.urihttps://doi.org/10.2478/v10042-010-0045-8
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4833
dc.identifier.volume48en_US
dc.identifier.wosWOS:000208226800005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherVia Medicaen_US
dc.relation.ispartofFolia Histochemica Et Cytobiologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNiO nanoparticlesen_US
dc.subjectcanceren_US
dc.subjecttoxicityen_US
dc.subjectapoptosisen_US
dc.titleCytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cellsen_US
dc.typeArticle

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