Yazar "Yigit, A. A." seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Age-and sex-related changes in selected hematological parameters, lipid peroxidation and erythrocytes osmotic fragility of Turkish Angora cats(Hellenic Veterinary Medical Soc, 2022) Kabakci, R.; Yigit, A. A.; Kara, E.; Cinar, M.; Ekici, H.; Aluc, Y.This study was conducted to investigate the changes in selected hematological parameters, lipid peroxidation and osmotic fragility of erythrocytes in Angora cats depending on age and gender. For this purpose, the blood samples were collected from vena saphena medialis of 9 young and 14 adult cats which were also classified as male (n=12) and female (n=11). Following hematological analysis, samples were washed with PBS by centrifugation and 10% hematocrit suspension was prepared from the erythrocytes pellet for the osmotic fragility test. The concentration of malondialdehyde (MDA) was also measured from lysed erythrocytes to determine lipid peroxidation level. Red blood cells (RBCs), hemoglobin, and hematocrit were significantly (P<0.001) high in adults while the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were significantly (P<0.05) high in young cats. Erythrocyte MDA level was also higher statistically (P<0.05) in adult cats than in young cats. There was no significance (P>0.05) in these parameters between male and female cats. Findings of fragility tests showed that erythrocytes of young and male cats were statistically more susceptible to hypotonic NaCl solutions than those of adult and female cats, respectively (P<0.01, P<0.05). It was concluded that erythrocytes related parameters in Angora cats changed depending on age rather than gender except for stability of RBCs.Öğe The avian embryo and its antioxidant defence system(Taylor & Francis Ltd, 2014) Yigit, A. A.; Panda, A. K.; Cherian, G.During chick embryo development, there exists an antioxidant-oxidant balance in the tissues, which supports normal embryonic development and post-hatch chick viability. This balance is maintained by natural antioxidants including vitamins A, E, C and carotenoids, glutathione peroxidase (GSH-PX), catalase (CAT) and superoxide dismutase (SOD). Chick viability is an important factor in determining post-hatch health and profitability. Factors that interrupt growth and development during the embryonic period affect the overall performance and health during the post-hatch period. During the 21-day incubation period for chickens, antioxidant defence systems protect the embryo against the lipid peroxidation (LPO) derived from undesirable conditions. The purpose of this review is to explain the antioxidant mechanisms that contribute to chick embryo development resulting in a healthy hatchling.Öğe Effect Of Bisphenol A And Diethylhexyl Phthalate On Progesterone Secretion By Luteal Cells(Wiley, 2017) Kabakci, R.; Yigit, A. A.…Öğe The effects of levamisole on oxidative stress induced by copper intoxication in broilers(Taylor & Francis Ltd, 2012) Yigit, A. A.; Cinar, M.; Yildirim, E.AIM: To determine the effects of Cu and levamisole on concentrations of Cu and Fe in plasma and liver, and the effects of levamisole on lipid peroxidation induced by Cu intoxication in broiler chickens. METHODS: In a 2x4 factorial study, 80 one-day-old Ross PM3 broiler chicks were fed diets for 21 days containing either 8 mg/kg Cu (Low Cu) or 250 mg/kg Cu (High Cu) and were treated with 0 (L0), 4 (L4), 8 (L8) or 16 (L16) mg/kg bodyweight levamisole per day from Day 7 of the study, on three consecutive days in their drinking water. This treatment was repeated three times, at 3-day intervals. On Day 21, blood samples were collected from each bird for analysis of concentrations of Cu, Fe and malondialdehyde, and activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), superoxide dismutase, catalase and glutathione peroxidase (GSH-Px). The birds were killed and liver samples collected for analysis of Cu and Fe. RESULTS: Mean concentrations of Cu and Fe in plasma, and Cu in liver, were increased overall in the High Cu groups compared with the Low Cu groups (p<0.001). Compared with the L0 treatment group on the High Cu diet, treatments L4, L8 and L16 decreased concentrations of Cu in plasma, and L8 and L16 increased concentration of Cu in liver (p<0.05). Mean activities of AST and ALT were increased in untreated birds (L0) fed the High compared with Low Cu diets (p<0.01). In birds receiving the High Cu diet, treatments L4 and L8 decreased activities of AST, and L4 and L16 decreased activity of ALT, compared with L0 (p<0.05). The High Cu diet induced an oxidative stress characterised by increased mean concentrations of malondialdehyde and decreased activities of superoxide dismutase, catalase and GSH-Px (p<0.001). Concentration of malondialdehyde, and activities of superoxide dismutase and catalase were not changed following levamisole treatment in birds on the High Cu diet, and activity of GSH-Px was decreased by the L4 and L8 treatments compared with the L0 group. CONCLUSIONS AND CLINICAL RELEVANCE: The results of the study suggest that treatment with levamisole might alleviate the harmful effects of Cu on the liver, as demonstrated by decreased activities of AST and ALT induced by a diet containing 250 mg/kg Cu.Öğe The effects of magnesium sulphate on the contractile activity of uterus in an animal model of preeclampsia(7847050 Canada Inc, 2014) Yildirim, E.; Macun, H. C.; Cinar, M.; Yigit, A. A.; Ustuner, I.; Aydos, T. R.; Aktuna, Z.Purpose: This study was undertaken to evaluate the effects of magnesium sulfate (MgSO4) on the contractile activity of the uterus in a pregnant rat model of preeclampsia induced by N-nitro-arginine methyl ester (L-NAME). Materials and Methods: Twenty-eight, 160220 gram, three to four month old female Sprague-Dawley rats were used in this study. After conception was confirmed by vaginal smears on the first day of pregnancy, the animals were allocated into four groups according to the chemicals fed in their drinking water as control (nothing administered), L-NAME (50 mg/kg L-NAME), MgSO4 (600 mg/kg MgSO4), and MgSO4 + L-NAME group (600 mg/kg MgSO4 + 50 mg/kg L-NAME). The pregnant uterus strips were isolated on the 19th day and the contractile activity of uterus was examined by applying 0, 0.1, 0.2, 0.4, 0.8, and 2.5 mIU/mloxytocin to each group and responses are recorded accordingly. Results: There were no statistically significant differences regarding fetal parameters and peak amplitudes of the oxytocin stimulated pregnant rat myometrial strips among groups. In L-NAME group at 0 and 0.1 mIU/ml oxytocin, the contraction frequency in a ten-min period was statistically lower than the control group (Z = -2.850, p = 0.004; Z = -2.902, p = 0.004, respectively). In MgSO4 group only at 0 mIU/ml oxytocin, the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -2.973,p = 0.003). In L-NAME + MgSO4 group at 0, 0.1 and 0.2 mIU/ml oxytocin concentrations the frequencies of the contractions in ten-min period were statistically lower than the control group (Z = -4.018, p = 0.000; Z= -3.23'7,p = 0.001; Z= -2.902, p = 0.004, respectively). In L-NAME + MgSO4 given group at each oxytocin concentrations, the frequencies of the contractions in ten-min period were lower but not statistically different than the L-NAME group. Conclusion: MgSO4 has no significant effect on the amplitude of spontaneous or oxytocin induced myometrial contractions, but decreased the frequency of spontaneous contractions. At each doses of oxytocin, MgSO4 has no significant effect on the frequency of contraction in a pregnant rat model of preeclampsia induced by L-NAME.Öğe The effects of the luteal cells cocultured with islet cells on cell viability and functionality in rats(Wiley, 2017) Yigit, A. A.; Boyuk, G.…Öğe Size Distribution of Luteal Cells During Pseudopregnancy in Domestic Cats(Wiley, 2009) Arikan, S.; Yigit, A. A.; Kalender, H.Contents Experiments were designed to investigate the size distribution of queen steroidogenic luteal cells throughout pseudopregnancy. Corpora lutea were obtained from the queens following ovariohysterectomy on days 7, 15 or 25 of pseudopregnancy. Luteal cells were isolated from the ovary by collagenase digestion. Steriodogenic cells were identified by staining of cells for 3 beta-HSD activity. Cell diameters were measured using a microscope. Luteal cells having steroidogenic capacity covered a wide spectrum of sizes ranging from 3 to 35 mu m in diameter. There was a significant increase in mean cell diameters (p < 0.01) as pseudopregnancy progressed. Mean diameter of 3 beta-HSD positive cells increased from 10.41 +/- 0.7 mu m, on day 7 of pseudopregnancy, to 19.72 +/- 1.3 mu m on day 25 of pseudopregnancy. The ratio of large (> 20 mu m in diameter) to small (3-20 mu m in diameter) luteal cells was 0.08 : 1.0 on day 7 of pseudopregnancy, with the 7.5-10 mu m cell size class predominant. By day 25 of pseudopregnancy, the ratio of large-to-small cells was increased to 0.87 : 1.0, and 20-25 mu m cell sizes become predominant. In conclusion, this study has demonstrated that the cells of the corpus luteum undergo continuous differentiation during pseudopregnancy in queen. This study also demonstrates that luteal cells dissociated from pseudopregnant queen can be used as a model to study the physiology of corpus luteum in pregnant cats.Öğe Total oxidant and antioxidant activities in milk with various somatic cell count intervals during discrete cow and buffalo lactation periods(Wiley, 2019) Yigit, A. A.; Cinar, M.; Macun, H. C.; Ozenc, E.; Kabakci, R.; Yazici, E.; Kalender, H.[Abstract No tAvailable]Öğe Total oxidant and antioxidant activities in milk with various somatic cell count intervals during discrete cow and buffalo lactation periods(Indian Dairy Assoc, 2018) Yigit, A. A.; Cinar, M.; Macun, H. C.; Ozenc, E.; Kabakci, R.; Yazici, E.; Salar, S.This study first investigated the total oxidant and antioxidant capacity (TOC and TAC, respectively) in cow and buffalo milk, with various somatic cell count (SCC) levels, having the same lactation numbers(LN). Second, it determined whether there is an association between the TAC and TOC and the lactation number, for the same SCC level. Quarter milk samples, collected from Holstein cows and Anatolian buffaloes, were separated into SCC levels of <= 2x10(5), 2x10(5)-5x10(5), 5x10(5)-1x10(6) and >= 1x10(6) cells mL(-1) for cow milk, and <= 2x10(5), 2x10(5)-4x10(5), 4x10(5)-1x10(6) and >= 1x10(6) cells mL(-1), for buffalo milk. Next, each group was subdivided, according to the lactation number (cows: 1-2nd, 3-4th, 5-6th, buffaloes: 1-4th, 5-8th, 9-12th), and TOC and TAC of the milk were measured. For the same lactation numbers, TOC increased in the cow and buffalo groups with an SCCV >= 1x10(6) cells mL(-1) (p< 0.05). Conversely, TAC decreased in cow milk with an SCC > 5x10(5)cells mL(-1). In buffalo milk, TAC decreased in parallel with the increased SCC. Among the same SCC groups, TOC and TAC were not affected by the lactation number, in cow and buffalo milk. An increased SCC caused an increased TOC and decreased TAC level, for the same lactation number. No relation existed among TOC, TAC and lactation number, for the same SCC level. SCC may be used as an indicator of TOC and TAC in cow and buffalo milk.
