Size Distribution of Luteal Cells During Pseudopregnancy in Domestic Cats
Yükleniyor...
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Contents Experiments were designed to investigate the size distribution of queen steroidogenic luteal cells throughout pseudopregnancy. Corpora lutea were obtained from the queens following ovariohysterectomy on days 7, 15 or 25 of pseudopregnancy. Luteal cells were isolated from the ovary by collagenase digestion. Steriodogenic cells were identified by staining of cells for 3 beta-HSD activity. Cell diameters were measured using a microscope. Luteal cells having steroidogenic capacity covered a wide spectrum of sizes ranging from 3 to 35 mu m in diameter. There was a significant increase in mean cell diameters (p < 0.01) as pseudopregnancy progressed. Mean diameter of 3 beta-HSD positive cells increased from 10.41 +/- 0.7 mu m, on day 7 of pseudopregnancy, to 19.72 +/- 1.3 mu m on day 25 of pseudopregnancy. The ratio of large (> 20 mu m in diameter) to small (3-20 mu m in diameter) luteal cells was 0.08 : 1.0 on day 7 of pseudopregnancy, with the 7.5-10 mu m cell size class predominant. By day 25 of pseudopregnancy, the ratio of large-to-small cells was increased to 0.87 : 1.0, and 20-25 mu m cell sizes become predominant. In conclusion, this study has demonstrated that the cells of the corpus luteum undergo continuous differentiation during pseudopregnancy in queen. This study also demonstrates that luteal cells dissociated from pseudopregnant queen can be used as a model to study the physiology of corpus luteum in pregnant cats.
Açıklama
Anahtar Kelimeler
Kaynak
Reproduction In Domestic Animals
WoS Q Değeri
Q1
Scopus Q Değeri
Q2
Cilt
44
Sayı
5
Künye
closedAccess
