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Öğe Analysis of Genetic Polymorphism with Microsatellite Method in Turkey Local Sheep Breeds(Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Yildiran, F. Azize Budak; Cakir, SukranIn this study, genetic relationships within and between the sheep breeds of Turkey called Bafra, Ivesi, Karayaka, Morkaraman, Sakiz and Turkish Merino was determined by microsatellite method. All breeds were screened for OarCP34, MAF65, MAF209 and DYMS1 loci by using ABI PRISM 310 sequencer. The obtained data were evaluated by using "Genetix 4.05" and "Populations 1.0" statistics programs. It was determined that the Turkish Merino breed had the highest heterozygosity. The shortest genetic distance was found between Sakiz and Morkaraman breeds and the longest genetic distance was observed between Turkish Merinos and Ivesi sheep breeds. As a result, in this study, genetic characterization in Turkey Local Sheep Breeds called Bafra, Sakiz, Karayaka, Ivesi and Turkish Merino breeds were made by using microsatellite markers.Öğe The Molecular Characterization of Echinococcus granulosus Isolates from Sheep in Kirikkale Region(Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Yildiran, F. Azize Budak; Yildiz, Kader; Cakir, Suekran; Gazyagci, Aycan N.In present study, it was aimed to determine molecular characterization of Echinococcus granulosus obtained from sheep. For this purpose, DNA isolation was done from germinal membrans and protoscolices of 24 hydatic cyst samples obtained from different organs of infected sheep and then DNA samples were scanned by RAPD-PCR using 16 primers. Echinococcus granulosus G1 strain was used as reference material. Only 4 of these 16 primers (AP2, AP4, OPB12 and G05) gave the typical RAPD band patterns in both sheep isolates and the reference G1 strain. The single band of 250 bp occurred by AP2 primer was common in both sheep isolates and G1strain According to result, AP2 primer can be successfully used for separation of G1 strain in different isolates. The results indicated that all sheep isolates sampled in this study can be assumed in the same Echinococcus granulosus strain.