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Öğe The therapeutic effects of coenzyme Q10 on surgically induced endometriosis in Sprague Dawley rats(Taylor & Francis Inc, 2022) Akarca-Dizakar, Saadet Özen; Demirel, Mürşide Ayşe; Coşkun Akçay, Neslihan; Sipahi, Mehmet; Karakoç Sökmensüer, Lale; Boyunaga, Hakan; Köylü, AyşeThe aim of this study was to evaluate the effects of coenzyme Q10 in the treatment of endometriosis rat models. Twenty seven Sprague Dawley rats were divided into four groups; Control Group (n = 7; Endometriosis group), Reference Group (n = 6; Endometriosis + Buserelin acetate, 20 mg/kg), CoQ10 Group-I (n = 7; Endometriosis + CoQ10, 50 mg/kg) and CoQ10 Group-II (n = 7; Endometriosis + CoQ10, 100 mg/kg). At the end of the experiment, all the rats were sacrificed, and the volume and histoarchitecture of endometrial implants were evaluated. The mast cells were determined by Toluidine blue and collagen fiber density was analysed by Masson's Trichrome staining. Tumour necrosis factor and vascular endothelial growth factor (VEGF) levels were analysed by enzyme-linked immunosorbent assay in peritoneal fluid and VEGF and matrix metalloproteinase-9 (MMP-9) were evaluated by immunohistochemistry. Terminal deoxynucleotidil transferase-mediated dUTP Nick end labelling (TUNEL) was also used for the detection of apoptotic cells. The CoQ10 treatment significantly decreased the volume of endometriotic implants, VEGF, and MMP-9 immunoreactivity and increased TUNEL-positive cells. The findings of the study suggest that CoQ10 can be used in endometriosis treatment by suppressing the endometriotic implants.IMPACT STATEMENT What is already known on this subject? Endometriosis is a gynaecological disorder and previous studies have shown that different treatments with antioxidants cause significant regression in the endometriotic implants. What the results of this study add? In this study, CoQ10 reduced intra-abdominal adhesion scores and volume of the endometriotic implants. In addition, CoQ10 treatment affected mast cell, TNF-alpha, VEGF, and MMP-9. What of these findings for clinical practice and/or further research? CoQ10 treatments may be possible to apply, it can contribute to science in terms of a new therapeutic treatment for endometriosis. Further studies are required to evaluate the Coenzyme Q10's effects on pain and subfertility in endometriosis.Öğe The prediction of separation-individuation through self-esteem in Turkish adolescents(Editura Univ Oradea, 2013) Asian, SevdaThis study determines the relationship between separation-individuation and self-esteem in terms of how Turkish late adolescents evaluate themselves with the help of the prediction of self-esteem. The study group consisted of students studying 9th, 10th, 11th, 12th grades in Anittepe High School, Ayranci High School, and Cumhuriyet High School. High-school form of the Separation-Individuation Test of Adolescence and Rosenberg Self-Esteem Scale were used as research instruments. The data gathered were analyzed with a multiple regression analysis method. The findings of this study revealed that self-esteem predicts separation anxiety, engulfment anxiety, teacher enmeshment, practicing mirroring, need denial, and rejection expectancy in a meaningful way. However, self-esteem does not predict nurturance seeking, peer enmeshment, and healthy separation. Research findings from the literature were discussed with those of the previous research and recommendations are presented.Öğe The effect of initial periodontal treatment on gingival crevicular fluid galectin-3 levels in participants with periodontal disease(Wiley, 2021) Karşıyaka Hendek, M.; Olgun, Ebru; Kısa, ÜçlerBackground: The aim of study was to evaluate galectin-3 levels in gingival crevicular fluid (GCF) from periodontally healthy (H) patients and those with periodontitis (P), gingivitis (G) and the effect of initial periodontal treatment on GCF galectin-3 level. Methods: A total of 75 participants, 25 patients with periodontitis, 25 with gingivitis and 25 periodontally healthy subjects were included into the study. Patients with periodontal disease received initial periodontal treatment. GCF galectin-3 level was assessed at baseline and at the 6th-8th weeks after completion of periodontal treatment. GCF galectin-3 level was evaluated by enzyme-linked immunosorbent assay. Results: GCF galectin-3 level was the lowest in the H group (102.31[63.07] mu g/30 s), followed by the G group (241.45[145.89] mu g/30 s) and the highest in the P group (338.27[219.37] mu g/30 s). These differences were statistically significant between H and the other groups (P < 0.001). After initial periodontal treatment, GCF galectin-3 level significantly decreased in the G and P groups compared to baseline values (P < 0.01). Conclusion: The results of this study suggest that GCF galectin-3 level is a potential biomarker for the evaluation of gingival inflammation and initial periodontal treatment is effective in decreasing GCF galectin-3 level.Öğe The effect of different irrigation solutions and activation techniques on the expression of growth factors from dentine of extracted premolar teeth(Wiley, 2021) Hançerlioğullari, Dilek; Erdemir, Ali; Kısa, ÜçlerAim To evaluate in a laboratory study, the effect of different canal irrigant solutions and activation techniques on transforming growth factor (TGF-beta 1), insulin growth factor-1 (IGF-1), bone morphogenetic protein-7 (BMP-7) and vascular endothelial growth factor-A (VEGF-A) release levels from the dentine of extracted premolar teeth. Methodology Seventy premolar teeth with single root and canal were used. The lengths of the root segments were standardized to 12 mm, and the root canals were prepared up to size 100 with hand files. All surfaces of the teeth were covered with nail polish except the inner root canal surface. The root canals were irrigated with 1.5% NaOCl. Ten teeth were allocated to the control group. The remaining sixty teeth were divided into 2 main groups according to the chelating agent used (17% EDTA, 10% Citric acid; CA) and 3 subgroups (n = 10) according to irrigation activation technique (conventional syringe irrigation (CSI), passive ultrasonic irrigation (PUI) and Er:YAG laser activation). After the activation procedure, the root segments were placed into eppendorf tubes containing 1 mL of phosphate-buffered saline solution and kept at 37celcius. TGF-beta 1, IGF-1, BMP-7 and VEGF-A release levels from dentine were measured using the enzyme-linked immunosorbent assay (ELISA) method at 24 h and at day 7. The volume of root canals was calculated using cone-beam computed tomography. The growth factor levels were calculated in ng/mL except VEGF-A (pg/ml). Normality analysis of the data was evaluated with the Kolmogorov-Smirnov test. Statistical analysis was performed using the Mann-Whitney-U and Wilcoxon tests. Results Regardless of the activation type and sampling time, EDTA caused significantly more IGF release than did CA, whereas EDTA and CA were equally effective for the release of the other growth factors. For either EDTA or CA, the lowest and highest growth factor release levels were observed in the CSI and Er:YAG laser groups, respectively (p < .05). All of the growth factors were released significantly more at 24 h than on day 7 (p < .05). Conclusions Irrigation activation techniques with EDTA or CA increased the release levels of all growth factors from the dentine of canal walls in extracted premolar teeth.Öğe The diagnostic value of neurogranin in patients with carbon monoxide poisoning: Can it show early neurological damage?(W B Saunders Co-Elsevier Inc, 2021) Yeşilyurt, Ömer; Cömertpay, Ertan; Vural, Sevilay; Eroğlu, Oğuz; Badem, Nermin Dindar; Çankaya, İmran; Bilgili, Yasemin KaradenizBackground and aim: Carbon monoxide poisoning is a toxicological emergency that causes neurological complications. High serum neurogranin can be detected in acute or chronic conditions where brain tissue is damaged. This study aimed to investigate the diagnostic value of serum neurogranin level and its role in demonstrating neurological damage in patients admitted to the emergency department with carbon monoxide poisoning. Materials and methods: The study was conducted prospectively on patients with carbon monoxide poisoning (patient group) and healthy volunteers (control group). Demographic characteristics and scrum neurogranin level of all participants and symptoms at admission, neurological examination findings, laboratory results, and Diffusion-Weighted Magnetic Resonance Imaging results of the patient group were recorded. We used an independent sample t-test to compare neurogranin levels and bivariate correlation analysis to compare the relationship between serum neurogranin levels and data belonging to the patient group. Results: Sixty eight participants (patient group, n- 36; control group, n- 32) were included in the study. Serum neurogranin level was significantly higher in patients with carbon monoxide poisoning (0.31 +/- 0.16 ng/ml) compared to control group (0.22 +/- 0.10 ng/ml) (p = 0.015). The mean Glasgow Coma Scale of the patients with carbon monoxide poisoning was 14.59 +/- 0.23, and of Diffusion Weighted Magnetic Resonance Imaging results were completely normal in 94.4% (n = 34). There was no correlation between serum neurogranin level and Diffusion Weighted Magnetic Resonance Imaging results (r = -0.011; p = 0.953). Conclusion: Serum neurogranin level may be a new diagnostic biomarker in patients admitted to the emergency department with carbon monoxide poisoning. The high serum neurogranin levels detected in patients with normal diffusion-weighted imaging after carbon monoxide poisoning suggest that there is neurological damage in these patients, even if imaging methods cannot detect it. (C) 2021 Elsevier Inc. All rights reserved.Öğe Tear Fluid and Serum Vitamin D Concentrations in Unilateral Pterygium(Lippincott Williams & Wilkins, 2021) Örnek, Nurgül; Oğurel, Tevfik; Kısa, ÜçlerSIGNIFICANCE Vitamin D has antiangiogenic, anti-inflammatory, and antifibrotic properties, which may play an inhibitory role on pterygium formation. Vitamin D concentration was measured in few studies, and contradictory results have been reported. There is no study investigating tear fluid concentration of vitamin D in pterygium patients. PURPOSE This study evaluated tear fluid and serum vitamin D concentrations of pterygium patients in comparison with healthy controls. METHODS Thirty-five (21 male, 14 female) patients with unilateral pterygium and 25 (18 male, 7 female) healthy controls were included in this case-control study. After full ophthalmic examination, blood samples were taken, and basal tear fluid was collected using glass microcapillary tubes. Tear fluid and serum vitamin D concentrations were analyzed using enzyme-linked immunosorbent assay and analyzed statistically. RESULTS The mean +/- standard deviation age of patients was 51.7 +/- 16.7 years in the study group and 50.6 +/- 18.7 years in the control group, respectively (P = .82). The mean tear fluid vitamin D concentration was statistically significantly higher than the mean serum concentration in the study groups (P < .0001). The mean tear fluid (P = .76) and serum vitamin D concentrations (P = .53) did not reveal statistically significant difference between patients and controls. There was no statistically significant difference for tear fluid vitamin D concentration between pterygium eyes and fellow eyes (P = .93). The difference in concentrations was compared within the pterygium subgroups, and it was found that the mean serum vitamin D concentration trended toward lower values as the stage of pterygium increased, and the mean tear fluid vitamin D concentration trended toward higher values as the stage of pterygium increased, although these differences were not statistically significant (all, P > .05). CONCLUSIONS Tear fluid and serum vitamin D concentrations do not seem to have a role in pterygium pathogenesis.Öğe Progranulin and tumor necrosis factor-alpha in lean polycystic ovary syndrome patients(Taylor & Francis Ltd, 2021) Uzdoğan, Andaç; Pekcan, Meryem Kuru; Çil, Aylin Pelin; Kısa, Üçler; Akbıyık, FilizObjective In this study, levels of progranulin (PGRN) and tumor necrosis factor-alpha (TNF-alpha) were measured to detect the presence of inflammation in lean polycystic ovary (PCOS) patients. Methods 40 lean PCOS patients were assessed by Rotterdam criteria. Forty healthy women with regular menstrual cycles and without biochemical and clinical hyperandrogenism were involved in our study. Blood samples were taken from the patient and control groups for the measurement of progranulin (PGRN), tumor necrosis factor-alpha (TNF-alpha), lipid parameters, glucose, insulin, and other hormones. Results Serum PGRN and TNF-alpha levels were significantly higher in patients with lean PCOS, compared with the control group (p = .037, p = .041). PGRN levels were positively correlated with TNF-alpha levels in lean PCOS patients. Conclusion PGRN is known as a ligand for the TNF-alpha receptor. PGRN level increase in lean PCOS patients may be due to inhibiting the inflammatory effects of TNF-alpha. To observe the PGRN and TNF-alpha connection in obesity, further study is needed in obese PCOS patients and obese control groups.Öğe Can Routine Blood Biochemistry Parameters be Predictive Prognostic Marker(s) in Operated Patients with Meningioma WHO Grade 1?(Georg Thieme Verlag Kg, 2021) Yüksel, Ulaş; Özdemir, Alemiddin; Kısa, Üçler; Ogden, Mustafa; Bakar, BülentBackground Today, there is a need for new and independent additional advanced markers that can predict the prognosis of meningioma patients, postoperatively. The present study aimed to find out postoperative short-term prognostic markers in patients with meningioma using their demographic data and routine blood biochemistry findings evaluated preoperatively. Methods The Glasgow Coma Scale (GCS), and Glasgow Outcome Scale (GOS) scores of the patients were recorded. Additionally, preoperatively obtained serum glucose, C-reactive protein (CRP), sodium, potassium, creatinine, blood urea nitrogen, aspartate aminotransferase (AST), alanine aminotransferase, and hemoglobin level values, platelet, leukocyte, neutrophil, lymphocyte, eosinophil, basophil, and monocyte count results, erythrocyte sedimentation rate (ESR), neutrophil-lymphocyte ratio, platelet-lymphocyte ratio (PLR) and lymphocyte-monocyte ratio (LMR) values were evaluated. Results In the present study, 23 operated patients with meningioma World Health Organization (WHO) grade 1 (17 females, 6 males) were included. Correlation test results revealed that the GCS score, platelet count, and serum potassium level values could directly predict the short-term prognosis of these patients. Additionally, these test results suggested that the lymphocyte, monocyte, and eosinophil count values, PLR, LMR, ESR, serum glucose, CRP, and AST level values could be indirect markers in predicting the short-term prognosis. However, likelihood ratio test results revealed that only monocyte count value, LMR value, and serum CRP level value could be the markers for prediction of the short-term prognosis. Conclusion At the end of the present study, it was concluded that the monocyte count value, LMR value, and serum CRP level value could be the best markers in predicting the short-term prognosis of the operated meningioma patients.Öğe Biochemical, pathological and ultrastructural investigation of whether lamotrigine has neuroprotective efficacy against spinal cord ischemia reperfusion injury(Elsevier Sci Ltd, 2021) Kahveci, Fatih Ozan; Kahveci, Ramazan; Gökçe, Emre Cemal; Gökçe, Aysun; Kısa, Üçler; Sargon, Mustafa Fevzi; Fesli, RamazanIntroduction: Lamotrigine, an anticonvulsant drug with inhibition properties of multi-ion channels, has been shown to be able to attenuates secondary neuronal damage by influencing different pathways. The aim of this study was to look into whether lamotrigine treatment could protect the spinal cord from experimental spinal cord ischemia-reperfusion injury. Materials and methods: Thirty-two rats, eight rats per group, were randomly assigned to the sham group in which only laparotomy was performed, and to the ischemia, methylprednisolone and lamot-rigine groups, where the infrarenal aorta was clamped for thirty minutes to induce spinal cord ischemia-reperfusion injury. Tissue samples belonging to spinal cords were harvested from sacrificed animals twenty-four hours after reperfusion. Tumor necrosis factor-alpha levels, interleukin-1 beta levels, nitric oxide levels, superoxide dismutase activity, catalase activity, glutathione peroxidase activity, malondialde-hyde levels and caspase-3 activity were studied. Light and electron microscopic evaluations were also performed to reveal the pathological alterations. Basso, Beattie, and Bresnahan locomotor scale and the inclined-plane test was used to evaluate neurofunctional status at the beginning of the study and just before the animals were sacrificed. Results: Lamotrigine treatment provided significant improvement in the neurofunctional status by pre -venting the increase in cytokine expression, increased lipid peroxidation and oxidative stress, depletion of antioxidant enzymes activity and increased apoptosis, all of which contributing to spinal cord damage through different paths after ischemia reperfusion injury. Furthermore, lamotrigine treatment has shown improved results concerning the histopathological and ultrastructural scores and the functional tests. Conclusion: These results proposed that lamotrigine may be a useful therapeutic agent to prevent the neuronal damage developing after spinal cord ischemia-reperfusion injury. (c) 2021 Elsevier Ltd. All rights reserved.Öğe Association Between Plasma Adrenomedullin Levels and Bone Mineral Density in Patients with Polycystic Ovary Syndrome(Galenos Yayincilik, 2005) Uçar, Banu; Noyan, Volkan; Yücel, Aykan; Sağsöz, Nevin; Çağlayan, OsmanObjective: The aim of the study was to compare bone mineral density measurements between patients with polycystic ovary syndrome (PCOS) and age and body mass index matched healthy controls, and to examine whether plasma adrenomedullin concentration was associated with bone mineral density. Design: Prospective study. Setting: Department of Obstetrics and Gynecology, Kirikkale University School of Medicine. Patients: Twenty women with PCOS and 13 healthy control subjects were enrolled in the study. Interventions: Plasma adrenomedullin, serum androstenedione, free testosterone, testosterone, DHEAS, SHBG, FSH, LH, estradiol, fasting insulin and fasting glucose were measured in each subject. Bone mineral density (lumbar spine 2-4 (L2-4), femoral neck, ward's triangle, great trochanter) measurements were measured using dual energy X-ray absorptiometry. Insulin resistance was estimated by fasting insulin level, fasting glucose: insulin ratio and 75 g of glucose tolerance test for 2 hours. Main Outcome Measures: Bone mineral density and correlation between bone mineral density and plasma adrenomedullin. Result: Bone mineral density measurements did not differ between the groups. There were no correlations between plasma adrenomedullin and bone mineral density measurements. Conclusions: Further studies are needed to evaluate the relation between bone mineral density and plasma adrenomedullin.Öğe A New Parameter for Erythropoiesis: The Relationship of Immature Reticulocyte Fraction Values in Cord Blood with Clinical Factors and Reference Values for Newborns(Duzce Univ, Fac Medicine, 2023) Dursun, Zarife Esra; Güzoğlu, Nilüfer; Kısa, Üçler; Davutoglu, Salih; Aliefendioglu, DidemAim: The immature reticulocyte fraction (IRF) is a new parameter for the reticulocyte maturity index, representing an independent parameter of erythropoiesis that may be useful to better assess erythropoietic activity in neonates. In this study, the relationship between IRF values and clinical features in newborns was investigated and reference values were obtained. Material and Methods: Newborns between 28-40 weeks of gestation were included in this prospective study. At birth, maternal venous and cord blood samples were obtained for measurements of complete blood count (CBC), blood gases, and plasma concentrations of various biochemical parameters. Results: A total of 123 newborns, 99 term and 24 preterm, were included in the study. When the laboratory characteristics of the premature and term babies were compared according to their gestational weeks, while the median IRF value of cord blood was higher in term babies than in premature babies (p=0.039), other laboratory findings did not differ significantly. The median IRF value was 0.52 (range, 0.15-1.00) in term infants and 0.34 (range, 0.16-0.76) in preterm infants. IRF reference values for the term and preterm newborns were determined in cord blood. Moderately positive correlations were observed between the IRF levels and both the RDW (r=0.423, p<0.001) and the CRP (r=0.389, p<0.001) levels. Conclusion: The results of this study showed that newborns' IRF values were not affected by maternal variables and changed with the week of birth. The results of this study might be considered a guide for future studies using IRF value in newborns.Öğe The Effects of Metformin, Ethinyl Estradiol/Cyproterone Acetate, and Metformin Ethinyl Estradiol/Cyproterone Acetate Combination Therapy on Carotid Artery Intima-media Thickness in Patients with Polycystic Ovary Syndrome(2020) Ünal, Derya; Demirci, Hüseyin; Yılmaz, Murat; Kısa, Üçler; Tulmaç, Murat; Güliter, SefaIntroduction: Patients with polycystic ovary syndrome (PCOS) are in the risk group for early-onset cardiovascular disease. There are few studies evaluating physiological and inflammatory cardiovascular risk factors in PCOS. Our study aimed to measure carotid intima-media thickness (IMT) in PCOS cases and to assess the effects of metformin, ethinyl estradiol/cyproterone acetate (EE/CA) and metformin + EE/ CA combination therapy on carotid IMT, insulin resistance, C-reactive protein (CRP), apelin and adiponectin. Methods: Basal carotid IMT, insulin resistance [Homeostasis model assessment insulin resistance (HOMA-IR)], apelin, adiponectin, and CRP values were evaluated in 60 women with PCOS and 43 healthy volunteers between the ages of 18 and 45. After baseline evaluation, patients were divided into metformin (n=20), EE/CA (n=20) and metformin + EE/CA (n=20) treatment groups. Treatment regimens were administered for six months. At the end of the treatment, the same parameters were reevaluated. Results: Compared with the control group, CRP (p=0.003), HOMA-IR (p=0.004) and IMT (p=0.049) were significantly higher, and adiponectin (p=0.002) and apelin (p=0.031) levels were significantly lower in patients with PCOS. At the end of the six-month treatment, the adiponectin level in the metformin (p=0.012) and metformin + EE/CA groups (p=0.012), and the apelin level in the metformin (p=0.024), EE/CA (p=0.024) and metformin + EE/CA groups (p=0.024) were significantly higher. There was no statistically significant change in CRP level in all treatment groups (p>0.05). There was no statistically significant change in carotid IMT value in all treatment groups (p>0.05). Conclusion: According to these results, we can say that women with PCOS have subclinical atherosclerosis and that metformin treatment has a positive effect on subclinical atherosclerosis.Öğe The effects of ozone on the acute phase of intestinal ischemia-reperfusion injury in rats(2020) Günal, Yasemin Dere; Türer, Özlem Boybeyi; Atasoy, Pınar; Kısa, Üçler; Aslan, Mustafa KemalBACKGROUND: In this study, we aimed to examine the therapeutic effects of ozone on the acute phase of intestinal ischemia-reperfusion (I/R) injury in rats to resemble clinical practice. METHODS: Eighteen Wistar albino rats were assigned to control (CG, n=6), sham (SG, n=6) and ozone groups (OG, n=6). A midline laparotomy was performed and a superior mesenteric artery (SMA) in the SG and OG was occluded with a 0/0 catgut suture, but in the CG, the incision was closed without any intervention. Tissue oxygenation was monitored with a tissue oxygenation monitor to achieve the same grade during intestinal ischemia. The incision was closed and, in the OG, ozone/oxygen mixture (0.7 mg/kg) was injected intraperitoneally, 20 minutes before reperfusion. Surgical incision was reopened and reperfusion was achieved after 60 minutes of ischemia in the SG and OG. After 60 minutes of reperfusion, 2 cm small intestine segment was sampled for histopathological assessment of the intestinal mucosal damage (Chiu score) and biochemical assessment of oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD) in all groups. RESULTS: The Chiu scores of the SG and OG were statistically increased than that of the CG (p=0.002; and p=0.002, respectively). Chiu score in the OG was higher compared to that in the SG, but not statistically significant (p=0.175). MDA levels were statistically higher in the SG and OG than that of the CG (p=0.004; and p=0.010, respectively). However, the difference between the SG and OG was not statistically significant (p=0.522). SOD and NO levels were not significantly different between groups (p=0.451 and p=0.056, respectively). CONCLUSION: Contrary to the literature, single-dose ozone therapy did not reduce the oxidative stress or improve the ischemic damage in intestinal I/R injury in rats. Further evaluation with different doses in different time periods is needed for potential clinical use.Öğe Kırıkkale Ve Çevresinde Ergen Bireylerde D Vitamini Düzeyi Değerlendirmesi(2019) Badem, Nermin DindarAmaç: Kırıkkale ve çevresinde yaşayan ergenlerde D vitamini düzeyininin yaş, cinsiyet ve ölçüm zamanına göre oluşturulmuş gruplardaki değişimini değerlendirmektir. Gereç ve Yöntem: Muhtelif endikasyonlarla D vitamini düzeyi ölçülmüş, yaşları 11-18 arasında 2672 ergen çalışmaya alındı. Ergenler yaş, cinsiyet ve ölçüm zamanına göre gruplara ayrıldı. D vitamini düzeylerine göre bireyler literatürlere uygun şekilde; D vitamini eksikliği (?20 ng/mL), D vitamini yetersizliği (21-29 ng/mL), optimal D vitamini düzeyi (30-100 ng/mL) şeklinde sınıflandırıldı. Bulgular: Çalışma grubunun 1747’si kızlardan (%65.4), 925’i erkeklerden (%34.6) oluşuyordu. Tüm grubun D vitamini düzeyi 15.54±8.59 ng/mL olmakla birlikte kızlarda 13.48±7.80 ng/mL, erkeklerde 19.42±8.67 ng/mL idi. Kız ve erkeklerin D vitamini düzeyleri anlamlı şekilde farklıydı (p<0.001). Kızların % 84.9’unda D vitamini eksikliği, % 12.1’inde D vitamini yetersizliği varken sadece % 3’ünde normal düzeyde D vitamini vardı. Bu sıralama erkeklerde % 59.5, % 31.4 ve % 9.2 şeklindeydi. D vitamini düzeyi kızlarda 11 yaştan 18 yaşa doğru daha da azalmakta olup her yaş grubunda erkeklerden anlamlı şekilde daha düşüktü. Aylara göre değerlendirmede özellikle temmuz, ağustos ve eylül aylarındaki D vitamini düzeyleri diğer aylara göre anlamlı şekilde yüksekti (p<0.001). Sonuç: Çalışmamızda ergenlik dönemindeki bireylerde D vitamini açısından yüksek oranda eksiklik/yetersizlik olduğunu ve bu eksikliğin her mevsimde görüldüğünü tespit ettik. Kızlarda bu eksiklik erkeklere göre daha belirgindi. Özellikle kızlara vitamin D takviyesi konusunda hassasiyet gösterilmelidir.Öğe Effects of thyroid hormone supplementation on oxidative stress after sleeve gastrectomy in rats(2018) Orman, Suleyman; Başok, Banu İşbilen; Kısa, Üçler; Kahraman, Nevin Genc; İşman, Ferruh KemalAim: Sleeve gastrectomy has been used for the surgical treatment of morbid obesity. The aim of the present study was to determine the effects of triiodothyronine supplementation on oxidative stress parameters in anastomotic tissue level. Material and Methods: Twenty-four male Wistar albino rats were divided into control (n:12), and experimental (n:12) groups and underwent sleeve gastrectomy. Experimental group rats received a single dose of triiodothyronine (400 mg/100 g) in the operation day. Rats were sacrificed on postoperative day 7. Serum thyroid hormones were analysed. The supernatants were used to measure total oxidant status, total antioxidant status, nitric oxide and malondialdehyde levels. All tissue parameters were analysed by spectrophotometric methods. Oxidative stress index values were calculated. Results: Thyroid stimulating hormone levels in both the control and triiodothyronine group did not significantly change on the 7th postoperative day (p=0.663). Free triiodothyronine levels were significantly higher in triiodothyronine group rats than in control group rats (triiodothyronine vs control: p=0.004). Although total oxidant status levels did not altered by thyroid hormone treatment (p>0.05), total antioxidant status levels significantly decreased (p<0.05). Oxidative stress index values were not statistically different in tissues (p>0.05). Tissue nitric oxide levels were also similar in both groups (p>0.05). Malondialdehyde levels increased in triiodothyronine given rats compared with the control group (p<0.01). Conclusion: This study showed that total oxidant status levels and oxidative stress index values were similar in both groups. However, triiodothyronine supplementation induced lipid peroxidation by increasing tissue malondialdehyde levels that might deplete tissue antioxidant level.Öğe Investigation (in vivo and in vitro) of booster dose vaccine requirement for long-term protection against hepatitis b virus infection(2009) Saygun, Onur; Eyigün, Can Polat; Avcı, İsmail Yaşar; Kısa, Üçler; Pahsa, AlaaddinAim: Studies have shown that no booster dose was required at least 10 to 15 years after a primary vaccination for individuals who developed protective anti-hepatitis B surface (anti-HBs) antibodies. In this study, booster dose requirement for HBV after primary immunization was investigated. Materials and Methods: Seventeen individuals vaccinated previously were enrolled in the study. They had once developed a protective level of anti-HBs antibody after immunization and their anti-HBs titer had declined to an underprotective level. Twenty uninfected and unvaccinated healthy people were chosen as controls. Lymphoproliferative response to in-vitro stimulation with hepatitis B surface antigen (HBsAg) and anti-HBs response to vaccine were evaluated for immune response. Results: T lymphocytes from 4 (24%) of the study group showed lymphoproliferative response to HBsAg stimulation while none of the controls did (P < 0.05). In all subjects in the study group, anti-HBs response (?10 mIU/ml) was detected 1 to 7 days after the booster injection but in only 2 of the controls antibody response was detected 28 days after the first dose of HBV vaccine (P < 0.0001). Conclusions: A booster dose of HBV vaccine might not be required because of immunological memory.Öğe The effect of insulin resistance on mortality in critically ill patients in the intensive care unit(INDIAN ANAESTHETISTS FORUM, 2020) Bakkal, Ayse Gulcan; Buyuksekerci, Murat; Gencay, Isin; Aydin, Gulcin; Caglayan, Osman; Buyukkocak, UnaseBackground and Aims: Insulin resistance can be described as a subnormal biological response to a specific insulin concentration or deterioration of an accepted response to insulin in glucose homeostasis and deficiency of insulin response. The aim of this study is to evaluate the effect of insulin resistance on mortality in critically ill patients. Methods: Over 18-year-old and nondiabetic 150 patients that had been hospitalized in an intensive care unit (ICU) between September 2013 and October 2014 were enrolled in this study. The Acute Physiology and Chronic Health Enquiry II (APACHE II), Glasgow Coma Scale, and Richmond Agitation and Sedation Scale were calculated on the day of admission to the ICU, and following 4th day and 1st, 2nd, 3rd, and 4th weeks. Insulin resistance was calculated using the HOMA formula. Infection and other complications during ICU stay, the requirement of mechanical ventilation (MV), nutritional status (parenteral and/or enteral), vasopressor, steroid, and insulin treatment were also recorded. Patients followed in the ICU were recorded as survivors and nonsurvivors. Results: Glucose levels were found to be higher in nonsurvivor group at the 1st week and there was a significant positive relationship between APACHE II score and insulin resistance at the 3rd week. There was a significant relationship between mortality and requirement of MV, vasopressor medication, complications, and infection. Conclusion: We conclude that the effect of insulin resistance seems to affect the mortality in critically ill patients after at least a 3 weeks long follow-up time.Öğe A new inflammatory marker in overweight patients: triggering receptor expressed on myeloid cells-1(COMENIUS UNIV, 2020) Cifci, A.; Ceylan, Durmaz S.; Oklu, K.; Gungunes, A.; Katar, D.; Karahan, I; Boyunaga, H.BACKGROUND: Triggering receptor expressed on myeloid cells 1 (TREM-1) is secreted by phagocytes in adipose tissue and it also upregulates the expression of genes involved in the inflammatory response and atherosclerotic conditions. This study was aimed to investigate the serum TREM-1 levels in overweight patients. METHODS: Twenty-eight subjects in the overweight group (OG) and 20 age-matched healthy subjects in the control group (CG) (BMI 27.6 +/- 1.2 vs 23.1 +/- 2.17 kg/m(2), respectively, p< 0.001) were included in the study. The serum sTREM-1 level was measured by ELISA. The homeostasis model assessment score (HOMA-IR) was also calculated. RESULTS: The mean TREM-1 levels were significantly higher in OG than in CG (407.3 +/- 323.7 vs 150.3 +/- 152.7 pg/mL, respectively, p< 0.001). The HOMA-IR score was also significantly higher in OG than in CG (3.42 +/- 3.63 vs 2.77 +/- 1.61, respectively). A positive correlation was detected between TREM-1 and BMI (r= 0.318, p= 0.028). CONCLUSIONS: This study mainly demonstrated that a high serum TREM-1 level might be an early inflammatory marker in overweight patients. We found that TREM-1 might be associated with BMI in overweight patients regardless of insulin resistance (Tab. 1, Ref. 21). Text in PDF www.elis.sk.Öğe The Role of Resolvin D1 in the Differential Diagnosis of the Cholangiocarcinoma and Benign Biliary Diseases(CLIN LAB PUBL, 2020) Gül-Utku, Özlem; Karatay, Eylem; Ergül, Bilal; Kısa, Üçler; Erdin, Züleyha; Oğuz, DilekBackground: The discrimination of malignant biliary strictures from benign biliary diseases (BBDs) is challenging and complicated. We aimed to investigate whether Resolvin D1 (RvD1) would aid in the discrimination of cholangiocarcinoma (CCA) from BBDs. Methods: Thirty-one patients with CCA, 27 patients with BBD, and 30 healthy controls were enrolled in this crosssectional study. The diagnosis of CCA was based on results obtained from abdominal USG, MRCP, abdominal CT, endosonography, and tumor markers, including CEA and CA 19-9. Histopathological evaluation was performed in the majority of patients, and the final diagnosis was based on surgery or biopsy results. RvD1, CEA, and CA 19-9 were analyzed in all patients with CCA and BBD. Results: RvD1 was significantly lower in those with CCA compared to patients with BBD and healthy controls. In addition, CEA and Ca 19-9 levels were significantly higher in the CCA group than the BBD group (p < 0.001). RvD1 concentration, CA 19-9 concentration, and total bilirubin level were found to be correlated with tumor stage (r = -0.702, 0.390, and 0.569, respectively). ROC curve analysis revealed that an RvD1 concentration of < 380 ng/mL (AUC: 0.783, 95% CI: 0673 - 0.893, p < 0.001) and CA 19-9 concentration of > 94.5 U/mL (AUC: 0.94, 95% CI: 0.898 - 0.998, p < 0.001) could be used to discriminate patients with CCA from those with BBD. Conclusions: Resolvin D1 and CA 19-9 levels might be used to effectively discriminate between BBD and CCA. Moreover, both RvD1 and CA 19-9 levels are associated with the stage of CCA, indicating that they may also be used in assessing disease progression.Öğe Prevention of the harmful effects of free oxygen radicals by using N-acetylcysteine in testicular torsion(ELSEVIER SCI LTD, 2020) Tangul, Sevgi Ulusoy; Cakmak, Ahmet Murat; Caglayan, Osman; Bozdogan, OnderIntroduction Testicular torsion is a urological emergency both in childhood and in adult life. Many studies on experimental testicular torsion have demonstrated biochemical and pathological ischemia reperfusion injury and the efficacy of some drugs have been investigated to prevent this damage. N-acetylcysteine (NAC) promotes glutathione synthesis and acts as a glutathione precursor because of the fact that it increases the glutathione-reductase activity by transporting sulfhydryl groups. Aim In this experimental study, the authors aimed to investigate the effectiveness of NAC in preventing ischemia-reperfusion injury following testicular torsion and detorsion. Study design For this experimental study, 36 albino Wistar-male rats were used. The rats were randomly divided into 4 groups: sham (n = 8), ischemia-reperfusion (n = 8), ischemia-NAC -reperfusion (n = 10), and ischemia-NAC-reperfusionNAC (n = 10) groups. Two hours of torsion and 4 h of detorsion were created in the left testis. After 4 h of detorsion, the rats were sacrificed. Each tissue was divided into two sections for biochemical and pathological examinations. Results There was a statistically significant difference between the study groups in terms of the total-sulfhydryl level, nitric oxide level, and the malondialdehyde values. Histopathological examination revealed that NAC was effective in preventing reperfusion injury in the testis but ineffective in preventing the reduction in the spermatid count. Discussion The results of this experimental study support that NAC can histopathologically maintain the structure of seminiferous tubules against ischemis reperfusion injury and prevent damage to the germinative cells. However, it was unable to prevent the reduction in spermatid count. There was no significant difference in the prevention of ischemiareperfusion injury between NAC administration during the first hour of ischemia and NAC administration during reperfusion. Although NAC can prevent tissue damage from ischemia reperfusion injury, it is not effective against the reduction in the spermatid count. Conclusion N-acetylcysteine may be biochemically effective in preventing ischemia-reperfusion injury after testicular torsion and detorsion. NAC is a readily available and easy to use agent that can be used during testicular ischemia.
