Diagnosis of acute brucellosis by polymerase chain reaction technique

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dc.contributor.authorÇeken, Sabahat
dc.contributor.authorKaygusuz, Sedat
dc.contributor.authorKiliç, Dilek
dc.contributor.authorAğalar, Canan
dc.date.accessioned2025-01-21T16:27:16Z
dc.date.available2025-01-21T16:27:16Z
dc.date.issued2015
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjective: Brucellosis is a zoonotic disease which is a public health problem in our country like many parts of the world. The illness has nonspecific symptoms and physical signs. Bacterial culture is gold standard in the diagnosis of brucellosis but it is difficult and time consuming, so serologic techniques are used routinely. But serologic techniques have low specificity because of cross reactions. Molecular methods like polymerase chain reaction (PCR) are good alternatives in the early and rapid diagnosis of brucellosis, as it is in other infectious diseases. The aim of this study was to compare PCR method with conventional methods in the diagnosis of brucellosis. Methods: The study included 35 patients and 20 healthy volunteers. Sera for serology and whole blood samples for PCR were collected from each subject in both groups. DNA was extracted from peripheral mononuclear cells obtained from the blood samples and an in house PCR assay was used to detect brucella DNA. Results: Standart tube agglutination (STA) titers of most patients were ?1/160, except one patient which was 1/80. Blood cultures were positive for 16 patients. The STA titers of all controls were negative. PCR was positive for 97% of the patients and all of the volunteers were negative. Conclusion: It is concluded that the tested PCR assay has high sensitivity in the diagnosis of brucellosis and it may be used in rapid and accurate diagnosis of brucellosis.
dc.identifier.doi10.5505/TurkHijyen.2015.60437
dc.identifier.endpage98
dc.identifier.issn0377-9777
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84937510098
dc.identifier.scopusqualityQ4
dc.identifier.startpage91
dc.identifier.urihttps://doi.org/10.5505/TurkHijyen.2015.60437
dc.identifier.urihttps://hdl.handle.net/20.500.12587/23308
dc.identifier.volume72
dc.indekslendigikaynakScopus
dc.language.isotr
dc.publisherRefik Saydam National Public Health Agency (RSNPHA)
dc.relation.ispartofTurk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectBrucellosis; Diagnosis; PCR
dc.titleDiagnosis of acute brucellosis by polymerase chain reaction technique
dc.title.alternativeAkut bruselloz tanısında polimeraz zincir reaksiyonu yönteminin kullanımı
dc.typeArticle

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