Investigation of Antioxidant, Antimicrobial and In Vitro Cytotoxic Effects on PC-3 Cancer Lines of Alchemilla holotricha Juz. in Different Polarities Extracts
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Tarih
2024
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The extracts of Alchemilla holotricha Juz. with different polarities were obtained using hexane, dichloromethane, ethyl acetate, methanol, and water. The samples were further analyzed for their overall antioxidant activity, ability to scavenge DPPH free radicals, capacity to chelate metals, total reducing power, ability to scavenging hydrogen peroxide, and hydroxy radical scavenging activity. The ethyl acetate extract exhibited the highest concentration of total phenols (67.63± 0.88 mg GAE/g extract), whereas both the ethyl acetate and dichloromethane extracts demonstrated the highest levels of total flavonoids (14.82±1.02-14.82±0.03 mg CE/g extract). On the other hand, the hexane extract displayed the highest content of phenolic acids (0.72±0.21 mg SAE/g extract). The antimicrobial activity of each extract was assessed using the agar disk diffusion method. The A. holotricha strain's ethyl acetate fraction displayed the most potent antibacterial activity. The Escherichia coli exhibited the greatest antibacterial impact, measuring 17 mm, while the Klebsiella pneumoniae showed a somewhat lower effect of 15 mm. Bacillus subtilis had the highest level of resistance among the microorganisms tested, with a diameter of 8 mm. A. holotricha displayed the most potent antioxidant and antibacterial properties in the ethyl acetate extracts. The study showed that ethyl acetate, a solvent with moderate polarity, is more efficient at dissolving aromatic compounds in the above-ground portion of the plant compared to non-polar solvents like n-hexane and diethyl ether. Moreover, the water and methanol extracts exhibited cytotoxicity towards PC-3 cells at particular concentrations.
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Kaynak
Türk Doğa ve Fen Dergisi
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Cilt
13
Sayı
3
