İneklerde östrus siklusu süresince gelişen steroidojenik ve non-steroidojenik luteal hücrelerin büyüklük dağılımlarında meydana gelen değişiklikler

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dc.contributor.authorYiğit, Ayşe Arzu
dc.contributor.authorArıkan, Şevket
dc.date.accessioned2021-01-14T18:16:17Z
dc.date.available2021-01-14T18:16:17Z
dc.date.issued2001
dc.description.abstractThe aim of this study was to investigate the size distribution of steroidogenic and non-steroidogenic luteal cells throughout the oestrus cycle. Twelve corpora lutea collected from different stages of the oestrus cycle were used. Corpora lutea collected from nonpregnant cows were dissociated into single-cell suspensions by enzyme treatments. Cells were stained for 3b-hydroxysteroid dehydrogenase (3b-HSD) activity, a marker for steroidogenic cells. The sizes of 3b-HSD positive (steroidogenic) and negative cells (nonsteroidogenic) were measured with an ocular micrometer. Very small cells (<10 µm) stained negative for 3b-HSD activity were presumed to be primarily endothelial cells, fibroblast and blood cells. The other cells were presumed to be luteal cells covering a wide spectrum of size ranging from 10 to 40 µm in diameter. 3b-HSD positive cells smaller than 10 µm were not observed, and cells larger than 25 µm were rare. The cells obtained from early and late stages of the luteal phase contained more non-steroidogenic cells than the cells obtained from the mid-luteal phase. However, the cells derived from early luteal stage contained more small cells (10-22 µm) than the cells obtained from the rest of the cycle. There were significant differences (p<0.001) in both 3b-HSD positive and 3b-HSD negative cell sizes during the oestrus cycle. This study indicates that the size distributions of both steroidogenic and non-steroidogenic luteal cells change as the age of the corpus luteum increases.en_US
dc.identifier.citationYiğit A. A., Arıkan Ş. (2001). İneklerde östrus siklusu süresince gelişen steroidojenik ve non-steroidojenik luteal hücrelerin büyüklük dağılımlarında meydana gelen değişiklikler. Turkish Journal of Veterinary and Animal Sciences, 25(4), 545 - 550.en_US
dc.identifier.endpage550en_US
dc.identifier.issn1300-0128
dc.identifier.issn1303-6181
dc.identifier.issue4en_US
dc.identifier.startpage545en_US
dc.identifier.trdizinid27976
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpjNU56WTI
dc.identifier.urihttps://hdl.handle.net/20.500.12587/13079
dc.identifier.volume25en_US
dc.indekslendigikaynakTR-Dizin
dc.language.isotr
dc.relation.ispartofTurkish Journal of Veterinary and Animal Sciences
dc.relation.publicationcategoryDiğeren_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectZiraat Mühendisliğien_US
dc.titleİneklerde östrus siklusu süresince gelişen steroidojenik ve non-steroidojenik luteal hücrelerin büyüklük dağılımlarında meydana gelen değişiklikleren_US
dc.title.alternativeChanges in the size distribution of steroidogenic and non-steroidogenic bovine luteal cells during the oestrus cycleen_US
dc.typeOther

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