Immobilization of laccase onto polyethyleneimine grafted chitosan films: Effect of system parameters

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dc.contributor.authorMetin, Aysegul U.
dc.date.accessioned2020-06-25T18:07:02Z
dc.date.available2020-06-25T18:07:02Z
dc.date.issued2013
dc.departmentKırıkkale Üniversitesi
dc.description.abstractChitosan films were prepared via phase-inversion technique. The polyethyleneimine (PEI) was grafted onto chitosan films after activation with various agents, such as epichlorohydrin, genipin and glutaraldehyde. The PEI grafted activated chitosan films (Ch-g-PEI) were used for immobilization of laccase by adsorption. The effects of adsorption parameters on immobilization efficiency were researched. Activity and stability of immobilized laccase on Ch-g-PEI films were investigated under desired optimum conditions. Characterization studies of the supports were also performed. The effect of pH on the activity of free and immobilized laccase was carried out in the pH range 4.0-8.0, and the optimum pH was determined as 5.5. Kinetic constants of the free and immobilized laccase were calculated. The maximum activity (V (max) ) and Michealis constant (K (m) ) of immobilized laccase on the films were found to be 71.2 U/mg and 0.51 mM, respectively. Thermal stability of the enzyme increased together with immobilization on the support. Free laccase retained 39% of its original activity at 55 A degrees C for a 120 min incubation period. Immobilized laccase retained 72% of its original activity for the same period at 55 A degrees C. The free enzyme lost all of its activity during the 7 weeks storage period. Conversely, immobilized laccase lost 36% of its activity during the 7 weeks storage period.en_US
dc.description.sponsorshipKirikkale UniversityKirikkale University [2009-46]en_US
dc.description.sponsorshipThis work was supported by the Kirikkale University Research Fund (2009-46). The author also would like to thank Professor Gulay Bayramoglu and Professor M. Yakup Arica for the use of Biochemical Processing and Biomaterial Research Laboratory, Gazi University Faculty of Natural Sciences and support.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s13233-013-1146-y
dc.identifier.endpage1152en_US
dc.identifier.issn1598-5032
dc.identifier.issn2092-7673
dc.identifier.issue10en_US
dc.identifier.startpage1145en_US
dc.identifier.urihttps://doi.org/10.1007/s13233-013-1146-y
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5452
dc.identifier.volume21en_US
dc.identifier.wosWOS:000322582600014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherPolymer Soc Koreaen_US
dc.relation.ispartofMacromolecular Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectchitosanen_US
dc.subjectepichlorohydrinen_US
dc.subjectgraftingen_US
dc.subjectpolyethyleneimineen_US
dc.subjectlaccaseen_US
dc.subjectimmobilizationen_US
dc.titleImmobilization of laccase onto polyethyleneimine grafted chitosan films: Effect of system parametersen_US
dc.typeArticle

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