Deneysel iskemik inme modelinde iskemi/reperfüzyon hasarına karşı sinapin tiyosiyanat ve/veya sinapik asit etkilerinin araştırılması
[ X ]
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kırıkkale Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
İnme; günlük hayatın artan stres düzeyi, beslenme alışkanlıklarının bozulması ve günlük hareket miktarının azalmasına bağlı olarak gelişebilen, insanlarda ve hayvanlarda yüksek oranda morbidite ve mortaliteye sahip olan bir hastalıktır. Bu çalışmanın amacı; iskemik inme modelinde iskemi/reperfüzyon hasarına karşı sinapin tiyosiyanat ve/veya sinapik asit'in etkileri araştırılmasıdır. Çalışmada; 56 Sprague-Dawley sıçan kullanıldı. Çalışma; Sham grubu, SA grubu ( 40 mg/kg SA), ST grubu ( 40 mg/kg ST) ve SA+ST grubu (40 mg/kg SA, 40 mg/kg ST), CCA grubu (iskemi/reperfüzyon), CCA+SA grubu (iskemi/reperfüzyon, 40 mg/kg SA, IP), CCA+ST grubu (iskemi/reperfüzyon, 40 mg/kg ST, IP), CCA+SA+ST grubu (iskemi/reperfüzyon, 40 mg/kg SA, 40 mg/kg ST, IP) olmak üzere 8 gruba (n:7) ayrıldı. Bu gruplardan; Sham grubu, SA grubu, ST grubu ve SA+ST grubuna madde uygulandıktan 3 saat sonra anestezi altındaki hayvanlar sakrifiye edildi. CCA (sağ ana karotis arter iskemi), CCA+SA, CCA+ST ve CCA+SA+ST gruplarındaki hayvanlar anestezi altında sağ ortak karotis arter klemplenerek inme oluşturuldu. İnme oluşturulan gruplardaki hayvanlara inmeden 60 dakika sonra intraperitoneal yolla madde uygulandı. İskemik inme oluşturulan gruplar; ilk 90 dakikalık inme süresi tamamlandıktan sonra sağ ana karotis arterdeki klemp açılarak iskemi/reperfüzyon hasarı oluşumu sağlandı. Reperfüzyondan 2,5 saat sonra; CCA, CCA+SA, CCA+ST ve CCA+SA+ST gruplarındaki hayvanlar anestezi altında sakrifiye edildi. Bütün gruplardaki hayvanların beyin dokusu çıkarıldı. Beyin dokusunda; TAS (total antioksidan durum), TOS (total oksidan durum), OSİ (oksidatis stres indeksi), MDA (Malondialdehit), GSH (glutatyon peroksidaz), GFAP (glial fibriler asidik protein) ve NSE (nöron spesifik enolaz) düzeyleri belirlendi. Ayrıca beyin dokusunun histopatolojik inceleme ve dejeneratif nöronların yüzdesi belirlendi. CCA grubunda OSI değeri diğer çalışma gruplarına kıyasla yüksek bulundu (p<0,001). CCA grubunda kıyasla CCA+SA, CCA+ST ve CCA+SA+ST gruplarında OSI değeri istatistiksel açıdan anlamlı olarak azaldı (p<0,001). MDA değeri; kontrol grubuna kıyasla CCA grubunda anlamlı düzeyde arttı (p<0,001). Diğer çalışma gruplarına kıyasla NSE ve GFAP düzeyleri CCA grubunda anlamlı düzeyde yüksek bulundu (p?0.001). CCA grubunda kıyasla CCA+SA, CCA+ST ve CCA+SA+ST gruplarında NSE düzeyi istatistiksel açıdan anlamlı olarak azaldı (p<0,001). GFAP düzeyi, CCA+SA, CCA+ST ve CCA+SA+ST gruplarında, CCA grubuna göre istatistiksel açıdan azaldığı gözlemlendi (p<0,001). Beyin korteksinde dejeneratif piramidal nöronların yüzdesi diğer gruplara kıyasla CCA grubunda istatistiksel olarak arttığı bulundu (p<0,001). Bu CCA grubundaki dejeneratif nöron yüzdesindeki artış, tedavi grupları olan CCA+SA, CCA+ST ve CCA+SA+ST gruplarında anlamlı bir şekilde azaldığı gözlendi (p<0,001). Sonuç olarak, SA, ST ve SA+ST uygulaması sıçanlarda serebral IR hasarı üzerinde önemli bir önleyici etkiye sahip olduğu bulundu. Bu bileşiklerin serebral IR hasarı üzerindeki koruyucu etkisinin çeşitli mekanizmalar ve doz-yanıt çalışmaları ile araştırılmasına odaklanmalıdır.
Stroke is a disease that can develop due to the increased stress level of daily life, deterioration of eating habits, and decreased daily activity, and has a high rate of morbidity and mortality in humans and animals. The purpose of this study; is to investigate the effects of sinapine thiocyanate and/or sinapic acid against ischemia/reperfusion injury in an ischemic stroke model. In the study, 56 Sprague-Dawley rats were used. In the study, they were divided into 8 groups (n:7): Sham group, SA group (40 mg/kg SA), ST group (40 mg/kg ST), and SA+ST group (40 mg/kg SA, 40 mg/kg ST), CCA group (ischemia/reperfusion), CCA +SA group (ischemia/reperfusion, 40 mg/kg SA, IP), CCA+ST group (ischemia/reperfusion, 40 mg/kg ST, IP), and CCA+SA+ST group (ischemia/reperfusion, 40 mg/kg SA, 40 mg/kg ST). From these groups, animals under anesthesia were sacrificed 3 hours after the substance was applied to the Sham group, SA group, ST group, and SA+ST group. Animals in the CCA, CCA+SA, CCA+ST, and CCA+SA+ST groups were stroked by clamping the right common carotid artery under anesthesia. The substance was administered intraperitoneally to the animals in the stroke-induced groups 60 minutes after the stroke. Groups created for ischemic stroke: After the first 90 minutes of stroke were completed, the clamp on the right common carotid artery was opened to ensure ischemia/reperfusion injury. 2.5 hours after reperfusion, animals in the CCA, CCA+SA, CCA+ST, and CCA+SA+ST groups were sacrificed under anesthesia. Brain tissue of animals in all groups was removed. The brain tissue of animals in all groups was removed. In brain tissue; TAS, TOS, OSI, MDA, GSH, GFAP, and NSE levels were determined. Additionally, the histopathological examination of brain tissue and the percentage of degenerative neurons were determined. The OSI value was found to be higher in the CCA group compared to other study groups (p<0.001). Compared to the CCA group, the OSI value decreased statistically significantly in the CCA+SA, CCA+ST, and CCA+SA+ST groups (p<0.001). MDA value; It increased significantly in the CCA group compared to the control group (p<0.001). Compared to other study groups, NSE and GFAP levels were found to be significantly higher in the CCA group (p?0.001). Compared to the CCA group, the NSE level decreased statistically significantly in the CCA+SA, CCA+ST, and CCA+SA+ST groups (p<0.001). It was observed that GFAP levels decreased statistically in the CCA+SA, CCA+ST, and CCA+SA+ST groups compared to the CCA group (p<0.001). The percentage of degenerative pyramidal neurons in the cerebral cortex was found to be statistically increased in the CCA group compared to the other groups (p<0.001). It was observed that the increase in the percentage of degenerative neurons in this CCA group decreased significantly in the treatment groups CCA+SA, CCA+ST, and CCA+SA+ST (p<0.001). In conclusion, SA, ST, and SA+ST administrations were found to have a significant preventive effect on cerebral IR injury in rats. The focus should be on investigating the protective effect of these compounds on cerebral IR injury through various mechanisms and dose-response studies.
Stroke is a disease that can develop due to the increased stress level of daily life, deterioration of eating habits, and decreased daily activity, and has a high rate of morbidity and mortality in humans and animals. The purpose of this study; is to investigate the effects of sinapine thiocyanate and/or sinapic acid against ischemia/reperfusion injury in an ischemic stroke model. In the study, 56 Sprague-Dawley rats were used. In the study, they were divided into 8 groups (n:7): Sham group, SA group (40 mg/kg SA), ST group (40 mg/kg ST), and SA+ST group (40 mg/kg SA, 40 mg/kg ST), CCA group (ischemia/reperfusion), CCA +SA group (ischemia/reperfusion, 40 mg/kg SA, IP), CCA+ST group (ischemia/reperfusion, 40 mg/kg ST, IP), and CCA+SA+ST group (ischemia/reperfusion, 40 mg/kg SA, 40 mg/kg ST). From these groups, animals under anesthesia were sacrificed 3 hours after the substance was applied to the Sham group, SA group, ST group, and SA+ST group. Animals in the CCA, CCA+SA, CCA+ST, and CCA+SA+ST groups were stroked by clamping the right common carotid artery under anesthesia. The substance was administered intraperitoneally to the animals in the stroke-induced groups 60 minutes after the stroke. Groups created for ischemic stroke: After the first 90 minutes of stroke were completed, the clamp on the right common carotid artery was opened to ensure ischemia/reperfusion injury. 2.5 hours after reperfusion, animals in the CCA, CCA+SA, CCA+ST, and CCA+SA+ST groups were sacrificed under anesthesia. Brain tissue of animals in all groups was removed. The brain tissue of animals in all groups was removed. In brain tissue; TAS, TOS, OSI, MDA, GSH, GFAP, and NSE levels were determined. Additionally, the histopathological examination of brain tissue and the percentage of degenerative neurons were determined. The OSI value was found to be higher in the CCA group compared to other study groups (p<0.001). Compared to the CCA group, the OSI value decreased statistically significantly in the CCA+SA, CCA+ST, and CCA+SA+ST groups (p<0.001). MDA value; It increased significantly in the CCA group compared to the control group (p<0.001). Compared to other study groups, NSE and GFAP levels were found to be significantly higher in the CCA group (p?0.001). Compared to the CCA group, the NSE level decreased statistically significantly in the CCA+SA, CCA+ST, and CCA+SA+ST groups (p<0.001). It was observed that GFAP levels decreased statistically in the CCA+SA, CCA+ST, and CCA+SA+ST groups compared to the CCA group (p<0.001). The percentage of degenerative pyramidal neurons in the cerebral cortex was found to be statistically increased in the CCA group compared to the other groups (p<0.001). It was observed that the increase in the percentage of degenerative neurons in this CCA group decreased significantly in the treatment groups CCA+SA, CCA+ST, and CCA+SA+ST (p<0.001). In conclusion, SA, ST, and SA+ST administrations were found to have a significant preventive effect on cerebral IR injury in rats. The focus should be on investigating the protective effect of these compounds on cerebral IR injury through various mechanisms and dose-response studies.
Açıklama
Sağlık Bilimleri Enstitüsü, Farmakoloji ve Toksikoloji (Veterinerlik) Ana Bilim Dalı
Anahtar Kelimeler
Veteriner Hekimliği, Veterinary Medicine
